HLAB (HLA-B) (NM_005514) Human Untagged Clone

SKU SC124484

HLA (untagged)-Human major histocompatibility complex, class I, B (HLA-B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HLAB
• Synonyms: AS; B-4901; HLAB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC124484 representing NM_005514.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGCTCTCGGCGGCCCTGGCCCTGACCGAGACCTGG; GCCGGCTCCCACTCCATGAGGTATTTCTACACCTCCGTGTCCCGGCCCGGCCGCGGGGAGCCCCGCTTC; ATCTCAGTGGGCTACGTGGACGACACCCAGTTCGTGAGGTTCGACAGCGACGCCGCGAGTCCGAGAGAG; GAGCCGCGGGCGCCGTGGATAGAGCAGGAGGGGCCGGAGTATTGGGACCGGAACACACAGATCTACAAG; GCCCAGGCACAGACTGACCGAGAGAGCCTGCGGAACCTGCGCGGCTACTACAACCAGAGCGAGGCCGGG; TCTCACACCCTCCAGAGCATGTACGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGCATGAC; CAGTACGCCTACGACGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCGCGGAC; ACGGCGGCTCAGATCACCCAGCGCAAGTGGGAGGCGGCCCGTGAGGCGGAGCAGCGGAGAGCCTACCTG; GAGGGCGAGTGCGTGGAGTGGCTCCGCAGATACCTGGAGAACGGGAAGGACAAGCTGGAGCGCGCTGAC; CCCCCAAAGACACACGTGACCCACCACCCCATCTCTGACCATGAGGCCACCCTGAGGTGCTGGGCCCTG; GGTTTCTACCCTGCGGAGATCACACTGACCTGGCAGCGGGATGGCGAGGACCAAACTCAGGACACTGAG; CTTGTGGAGACCAGACCAGCAGGAGATAGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGA; GAAGAGCAGAGATACACATGCCATGTACAGCATGAGGGGCTGCCGAAGCCCCTCACCCTGAGATGGGAG; CCGTCTTCCCAGTCCACCGTCCCCATCGTGGGCATTGTTGCTGGCCTGGCTGTCCTAGCAGTTGTGGTC; ATCGGAGCTGTGGTCGCTGCTGTGATGTGTAGGAGGAAGAGTTCAGGTGGAAAAGGAGGGAGCTACTCT; CAGGCTGCGTGCAGCGACAGTGCCCAGGGCTCTGATGTGTCTCTCACAGCTTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005514 unedited; CAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGAGATGCTG; GTCATGGCGCCCCGAACCGTCCTCCTGCTGCTCTCGGCGGCCCTGGCCCTGACCGAGACC; TGGGCCGGCTCCCACTCCATGAGGTATTTCTACACCTCCGTGTCCCGGCCCGGCCGCGGG; GAGCCCCGCTTCATCTCAGTGGGCTACGTGGACGACACCCAGTTCGTGAGGTTCGACAGC; GACGCCGCGAGTCCGAGAGAGGAGCCGCGGGCGCCGTGGATAGAGCAGGAGGGGCCGGAG; TATTGGGACCGGAACACACAGATCTACAAGGCCCAGGCACAGACTGACCGAGAGAGCCTG; CGGAACCTGCGCGGCTACTACAACCAGAGCGAGGCCGGGTCTCACACCCTCCAGAGCATG; TACGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGCATGACCAGTACGCCTAC; GACGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCGCGGACACG; GCGGCTCAGATCACCCAGCGCAAGTGGGAGGCGGCCCGTGAGGCGGAGCAGCGGAGAGCC; TACCTGGAGGGCGAGTGCGTGGAGTGGCTCCGCAGATACCTGGAGAACGGGAAGGACAAG; CTGGAGCGCGCTGACCCCCCAAAGACACACGTGACCCACCACCCCATCTCTGACCCATGA; GGCCACCCTGAGTGCTGGGCCCTGGGTTTCTACCCTGCNGAGATCACACTGACCTGGCAG; CGGGATGGCGAGGACCAAACTCANGACACTNGAGCTTGTGGAGACCCAGACCAGCANGAG; ATAGAACCTTCCANAAGNNTGGCAGCTGTGGTGGNTGCCTTNCTGAGAAGAGCAGAGATA; CACATGCCATGTACAGCATGAGGGGCTGCCGAGCCCCTCACCTGAGAGGNNAGCGTNNTC; CCAGTCACGTNCCATCGTGGGCATTGTGCTGGCCTGCTGCCTANCANTGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005514 unedited; NGGTACGACTATGGNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGA; AGGTTCTCAGGTCTTTATTTGCTCTCTCAAATTCCAGGAATTGACTTATTTAATTAATCC; ATCAACCTCTCATAGCAAATATTTGAGAAAACAAATTTATATTCAGATTCTTATTTTCAG; TAGGGAAGTAAGAAGTTGCAGCTCAGTGCACGTAAAGTTGAGACAGAGATGGAGACATCC; AGCCCCACCTCTCTGGAACAAGAAAGATGACTGGGGAGGAAACACAGGTCAGCATGGGAA; CAGGGGTCACAGTGGACACAAGGGTGGGCTGTCTCTCCACCTCCTCACATTATGCTAACA; GGGACGCAGACACATTCAGGTGCCTTTGCAGAAAGAGATGCCAGAGGCTCTTGAAGTCAC; AAAGGGGAGGCGTGAAGAAATCCTGCATCTCAGTCCCTCACAAGACAGCTGTCTCAGGCT; TTTCAAGCTGTGAGAGACACATCAGAGCCCTGGGCACTGTCGCTGCACGCAGCCTGAGAG; TAGCTCCCTCCTTTTCCACCTGAACTCTTCCTCCTACACATCACAGCAGCGACCACAGCT; CCGATGACCACAACTGCTAGGACAGCCAGGCCAGCAACAATGCCCACGATGGGGACGGTG; GACTGGGAAGACGGCTCCCTCTAGGTGGAGGGGGGCTTCGGCAGCCCCTCATGCTGTACA; TGGCATGTGTATCTCTGCTCTTCTCCAGAAGCACCACACAGCTGCCCACTCTGGAAGGGT; CTATCTCCTGGTGGTCTGGTCTCCCCAGCTCATGTCCTGAGTTTGGTCCTCGCCATCCGC; TGCCAGTCAGTGTGATCTCCGCAGGGTAGAAACCCAGGGCCAGCACCTTCAGGTGGCCCT; CTGGTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_005514
• Insert Size: 1089 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005514.5
• RefSeq Size: 1533 bp
• RefSeq ORF: 1089 bp
• Locus ID: 3106
• UniProt ID: P01889
• Cytogenetics: 6p21.33
• Domains: ig, IGc1, MHC_I
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis
• MW: 40.5 kDa
• Summary: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]