BDNF (NM_001709) Human Untagged Clone

CAT#: SC124461

BDNF (untagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BDNF
• Synonyms: ANON2; BULN2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124461 sequence for NM_001709 edited (data generated by NextGen Sequencing)
  ATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTTGGTTGCATGAAGGCTGCCCCC
  ATGAAAGAAGCAAACATCCGAGGACAAGGTGGCTTGGCCTACCCAGGTGTGCGGACCCAT
  GGGACTCTGGAGAGCGTGAATGGGCCCAAGGCAGGTTCAAGAGGCTTGACATCATTGGCT
  GACACTTTCGAACACGTGATAGAAGAGCTGTTGGATGAGGACCAGAAAGTTCGGCCCAAT
  GAAGAAAACAATAAGGACGCAGACTTGTACACGTCCAGGGTGATGCTCAGTAGTCAAGTG
  CCTTTGGAGCCTCCTCTTCTCTTTCTGCTGGAGGAATACAAAAATTACCTAGATGCTGCA
  AACATGTCCATGAGGGTCCGGCGCCACTCTGACCCTGCCCGCCGAGGGGAGCTGAGCGTG
  TGTGACAGTATTAGTGAGTGGGTAACGGCGGCAGACAAAAAGACTGCAGTGGACATGTCG
  GGCGGGACGGTCACAGTCCTTGAAAAGGTCCCTGTATCAAAAGGCCAACTGAAGCAATAC
  TTCTACGAGACCAAGTGCAATCCCATGGGTTACACAAAAGAAGGCTGCAGGGGCATAGAC
  AAAAGGCATTGGAACTCCCAGTGCCGAACTACCCAGTCGTACGTGCGGGCCCTTACCATG
  GATAGCAAAAAGAGAATTGGCTGGCGATTCATAAGGATAGACACTTCTTGTGTATGTACA
  TTGACCATTAAAAGGGGAAGATAG
  
  Clone variation with respect to NM_001709.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001709 unedited
  CGTAGCGCCTCGGGCTCCCGGGCCGGACAGAGGAGCCAGCCCGGTGCGCCCCTCCACCTC
  CTGCTCGGGGGGCTTTAATGAGACACCCACCGCTGCTGTGGGGCCGGCGGGGAGCAGCAC
  CGCGACGGGGACCGGGGCTGGGCGCTGGAGCCAGAATCGGAACCACGATGTGACTCCGCC
  GCCGGGGACCCGTGAGGTTTGTGTGGACCCCGAGTTCCACCAGGTGAGAAGAGTGATGAC
  CATCCTTTTCCTTACTATGGTTATTTCATACTTTGGTTGCATGAAGGCTGCCCCCATGAA
  AGAAGCAAACATCCGAGGACAAGGTGGCTTGGCCTACCCAGGTGTGCGGACCCATGGGAC
  TCTGGAGAGCGTGAATGGGCCCAAGGCAGGTTCAAGAGGCTTGACATCATTGGCTGACAC
  TTTCGAACACGTGATAGAAGAGCTGTTGGATGAGGACCAGAAAGTTCGGCCCAATGAAGA
  AAACATAAGGACGCAGACTTGTACACGTCCCAGGTGATGCTCAGTAGTCAAGTGCCTTTG
  GAGCCTCCTCTTCTTTTTCTGCTGGAGGAATACAAAAATTACCTAGATGCTGGAAACATG
  TCCATGAGGGTTCGGGGCCACTTTGACCTTGCCCGCCGAGGGCAGCTGACCGTGTCGGAC
  AGTTTTAGTGAGTGGGTCACCGGCCGTTAAACAATAGACTGAGTGGACATGTTGGACGGT
  ACGGCTCAAACCTTAGTAAGGCCCTGTTTCAAAGGCTACTGAATCTTTCTTTTACAATCC
  AGTGTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001709 unedited
  CTATGGAACGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATAATAAAAC
  ACGTTTTCATGTTTGTTTTACAGTAAAGAGAAAAAAACCCAGAACCCCCAGATTTTATGT
  ACTTTGAAAATATATTTAAAAACATTAAAAATTCTATATTTAAAACATATATTATATGTT
  AATTAGTACACTTAAATAGAACTTGTATTTACAATAGGCTTCTGATGCGGTTAAGTTTTA
  ATGCCAATTTTTTTCAATAACATAATTATATAAATATACTAAAATACAATAAATATTGTT
  TTTTGTTTTACATGGTGAATAATATCTTTACCATAGAGAGAACAAGGCCACAGACATTTA
  CTTACATTTTCAATGGGAATCGCCATAAAAAAGCAACAGGCCTGCTGCCATGCATGAAAC
  ACTTCTGCCACAAAGAGACCACAGCAAGACTTTAAAAAACAAAACAAAACAGAACAAGAA
  CGGACACAACAGAGAGAGATTTTAACAAAATAAATCTTAGGTCAACATAAACCATCAAGC
  ATGTGACTGTGATGTATCTTATTGGGTAAAAGAGCCACTGACCACACAATTGCTGGATGT
  GTCTCCTATGAAACCACTTAACAGATCTGGCCCTTGCAATCCTTTAAGTTTGTGATGGGG
  GGTTGTTTGTTTTAAACTTGCCCCTCAAAAGAAGCTGCCTAAAGTTGACACATACCACAT
  ATTCCAAGCATTTCCTTACATATTTAAAATAATTACAAAGATTATATTTTCAAACAACAC
  CAAACAAATCTACACTACGTAAAGTCTTTCCTTCTTTAAAGAAGTATTCAGATGGTACTG
  GGAAAGACTACCTTAAATGAAACGATTGACAAATTGAAATTTCTTGCTGATGGGATCTTC
  ATTCCTGGGGTATGGGCGAAATCNCTCTTTGGCTTATCCTACACCTACTAAGAGCAAGAA
  TTGCTGGCGGCTTTCTGAACCTAAAGAGCTGGTCCAGTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001709
• Insert Size: 4200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001709.3, NP_001700.2
• RefSeq Size: 3972 bp
• RefSeq ORF: 744 bp
• Locus ID: 627
• UniProt ID: P23560
• Cytogenetics: 11p14.1
• Domains: NGF
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane
• Protein Pathways: Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (4), also known as VIb or BDNF5, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a).