14 3 3 gamma (YWHAG) (NM_012479) Human Untagged Clone

CAT#: SC124450

YWHAG (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 14 3 3 gamma
• Synonyms: 14-3-3GAMMA; DEE56; EIEE56; PPP1R170
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124450 sequence for NM_012479 edited (data generated by NextGen Sequencing)
  ATGGTGGACCGCGAGCAACTGGTGCAGAAAGCCCGGCTGGCCGAGCAGGCGGAGCGCTAC
  GACGACATGGCCGCGGCCATGAAGAACGTGACAGAGCTGAATGAGCCACTGTCGAATGAG
  GAACGAAACCTTCTGTCTGTGGCCTACAAGAACGTTGTGGGGGCACGCCGCTCTTCCTGG
  AGGGTCATCAGTAGCATTGAGCAGAAGACATCTGCAGACGGCAATGAGAAGAAGATTGAG
  ATGGTCCGTGCGTACCGGGAGAAGATAGAGAAGGAGTTGGAGGCTGTGTGCCAGGATGTG
  CTGAGCCTGCTGGATAACTACCTGATCAAGAATTGCAGCGAGACCCAGTACGAGAGCAAA
  GTGTTCTACCTGAAGATGAAAGGGGACTACTACCGCTACCTGGCTGAAGTGGCCACCGGA
  GAGAAAAGGGCGACGGTGGTGGAGTCCTCCGAGAAGGCCTACAGCGAAGCCCACGAGATC
  AGCAAAGAGCACATGCAGCCCACCCACCCCATCCGATTAGGCCTGGCTCTTAACTACTCC
  GTCTTCTACTATGAGATCCAGAACGCCCCAGAGCAAGCGTGCCACTTGGCCAAGACCGCG
  TTCGACGACGCCATCGCCGAGCTTGACACCCTCAACGAGGACTCCTACAAGGACTCCACG
  CTCATCATGCAGCTCCTCCGCGACAACCTCACGCTCTGGACGAGCGACCAGCAGGACGAC
  GATGGCGGCGAAGGCAACAATTAA
  
  Clone variation with respect to NM_012479.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012479 unedited
  AGGGCGGCCGCGTAATTCGCACGAGGGCGCAGCCGCCGCCTCAGCTGCTCGCTCTGTGGG
  TCGGTCCTCTCCGGCACTTGGGCTCCAGTCGCGCCCTCCAAGCCCTTCAGGCCGCCCCAG
  TGTCCTCCTCCTTCTCCGGCCAGACCCAGCCCCGCGAAGATGGTGGACCGCGAGCAACTG
  GTGCAGAAAGCCCGGCTGGCCGAGCAGGCGGAGCGCTACGACGACATGGCCGCGGCCATG
  AAGAACGTGACAGAGCTGAATGAGCCACTGTCGAATGAGGAACGAAACCTTCTGTCTGTG
  GCCTACAAGAACGTTGTGGGGGCACGCCGCTCTTCCTGGAGGGTCATCAGTAGCATTGAG
  CAGAAGACATCTGCAGACGGCAATGAGAAGAAGATTGAGATGGTCCGTGCGTACCGGGAG
  AAGATAGAGAAGGAGTTGGAGGCTGTGTGCCAGGATGTGCTGAGCCTGCTGGATAACTAC
  CTGATCAAGAATTGCAGCGAGACCCAGTACGAGAGCAAAGTGTTCTACCTGAAGATGAAA
  GGGGACTACTACCGCTACCTGGCTGAAGTGGCCACCGGAGAGAAAAGGGCGACGGTGGTG
  GGAGTCCTCTGAGAAGGCCTACAGCGAAGCCCACGAGATCAGCAAAGAGCACATGCAGCC
  CACCCACCCCATCCGATTAGGCCTGGCTCTTAACTACTNCGTCTTCACTATGAGATCCAG
  AACGCCCCAGAGCAGCGTGCCACTTGCCAAGACCGCGTTCGACGAGCCATCGNCGAGCTT
  GACACCCTAACGAAGACTCTACAAGGACTNCCGCTCATATGCAGNTCCTCGNGACAACTC
  CGCTCTGGAGAGCGACANCAGACNANATGGCGGCAAGCACATTAAGCCCAGGGACTGCAC
  GC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012479 unedited
  GTACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTCAAATCTGTAAGTTTATTTGCTCAATGTACGACAGCTACATAATGACTCACAT
  TCATGATATTCCATCACTGAGGAAACTGCTAAAGATGGTCCGTGTGTGAAATAATTCCTT
  AAAGAAACACGGAGCTGGAAAAATAATCACTGATTAGACCTTAAAAATAGTTCACTGCAT
  AACATGACAAAAAGCACAAAGGCTCATTCAGAGAACATATTTGTTGTTCTCCAACCCTGT
  AATAGTTATAATTTCACCATGACAAACACCAGACATTAAGATTAAGCTAACCCTGGTGTT
  TCTTTTGCTCCCCCCCTTTTAAAAACAAAATATATAACTGCATGTCACTATAGCAACATC
  CAAAACAGATCAATTTGTTACAATCACTATTTGGTAGAGCAAACTTTACCCCCAAAAGGA
  AAAATTAAATTAAAAAAAAAAAACCTTTAAAAAATTTGAAGACTTAATTTTTTTTGTCAT
  AGGAATACATAACACCTACAGTATAAGTTAATCAATTTCAAGCTACTGTATAGAAATACA
  ACACTAGCATGCACAATATTGTATCAATAGAGTAATGGAGGAGTAATCATTTCCCTGGAG
  AGACAGTTCATAGGCCAGTGCATTTCTTTAAAAATAAAGAAAAGAAAAACCATTTCAGGC
  TGCTTTAATATCAAGTCTCCCATTCCCTCTCCCGTTTCAGCCCCTCAGTGGGGATTTTTA
  TCTTGCCTTATTCTAAAAAGCAGCCGGGAGCCAAAGCTGGAATTTTAAGGAAAAATAGTT
  TTGTTATTTCAGTAAATCATTTCAAATGCTACAGGGAAAACCCACCTTGCTTCTTGGACT
  GAAGATAATATGGGAAAGCCCCCTTCCGTGCGCCCCCCCCCCCCCCCCCNNAAAATAATT
  TTTCNTAGGGAAGCTTTGGGGCGGGGAGGAGCCAAAACCGGTGAGTTTTTTTTCTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_012479
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012479.2, NP_036611.2
• RefSeq Size: 3747 bp
• RefSeq ORF: 744 bp
• Locus ID: 7532
• UniProt ID: P61981
• Cytogenetics: 7q11.23
• Domains: 14-3-3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
• Gene Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]