14 3 3 gamma (YWHAG) (NM_012479) Human Untagged Clone

SKU SC124450

YWHAG (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG)

Product Data

• Type: Human Untagged Clone
• Target Symbol: 14 3 3 gamma
• Synonyms: 14-3-3GAMMA; DEE56; EIEE56; PPP1R170
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124450 sequence for NM_012479 edited (data generated by NextGen Sequencing); ATGGTGGACCGCGAGCAACTGGTGCAGAAAGCCCGGCTGGCCGAGCAGGCGGAGCGCTAC; GACGACATGGCCGCGGCCATGAAGAACGTGACAGAGCTGAATGAGCCACTGTCGAATGAG; GAACGAAACCTTCTGTCTGTGGCCTACAAGAACGTTGTGGGGGCACGCCGCTCTTCCTGG; AGGGTCATCAGTAGCATTGAGCAGAAGACATCTGCAGACGGCAATGAGAAGAAGATTGAG; ATGGTCCGTGCGTACCGGGAGAAGATAGAGAAGGAGTTGGAGGCTGTGTGCCAGGATGTG; CTGAGCCTGCTGGATAACTACCTGATCAAGAATTGCAGCGAGACCCAGTACGAGAGCAAA; GTGTTCTACCTGAAGATGAAAGGGGACTACTACCGCTACCTGGCTGAAGTGGCCACCGGA; GAGAAAAGGGCGACGGTGGTGGAGTCCTCCGAGAAGGCCTACAGCGAAGCCCACGAGATC; AGCAAAGAGCACATGCAGCCCACCCACCCCATCCGATTAGGCCTGGCTCTTAACTACTCC; GTCTTCTACTATGAGATCCAGAACGCCCCAGAGCAAGCGTGCCACTTGGCCAAGACCGCG; TTCGACGACGCCATCGCCGAGCTTGACACCCTCAACGAGGACTCCTACAAGGACTCCACG; CTCATCATGCAGCTCCTCCGCGACAACCTCACGCTCTGGACGAGCGACCAGCAGGACGAC; GATGGCGGCGAAGGCAACAATTAA; ; Clone variation with respect to NM_012479.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012479 unedited; AGGGCGGCCGCGTAATTCGCACGAGGGCGCAGCCGCCGCCTCAGCTGCTCGCTCTGTGGG; TCGGTCCTCTCCGGCACTTGGGCTCCAGTCGCGCCCTCCAAGCCCTTCAGGCCGCCCCAG; TGTCCTCCTCCTTCTCCGGCCAGACCCAGCCCCGCGAAGATGGTGGACCGCGAGCAACTG; GTGCAGAAAGCCCGGCTGGCCGAGCAGGCGGAGCGCTACGACGACATGGCCGCGGCCATG; AAGAACGTGACAGAGCTGAATGAGCCACTGTCGAATGAGGAACGAAACCTTCTGTCTGTG; GCCTACAAGAACGTTGTGGGGGCACGCCGCTCTTCCTGGAGGGTCATCAGTAGCATTGAG; CAGAAGACATCTGCAGACGGCAATGAGAAGAAGATTGAGATGGTCCGTGCGTACCGGGAG; AAGATAGAGAAGGAGTTGGAGGCTGTGTGCCAGGATGTGCTGAGCCTGCTGGATAACTAC; CTGATCAAGAATTGCAGCGAGACCCAGTACGAGAGCAAAGTGTTCTACCTGAAGATGAAA; GGGGACTACTACCGCTACCTGGCTGAAGTGGCCACCGGAGAGAAAAGGGCGACGGTGGTG; GGAGTCCTCTGAGAAGGCCTACAGCGAAGCCCACGAGATCAGCAAAGAGCACATGCAGCC; CACCCACCCCATCCGATTAGGCCTGGCTCTTAACTACTNCGTCTTCACTATGAGATCCAG; AACGCCCCAGAGCAGCGTGCCACTTGCCAAGACCGCGTTCGACGAGCCATCGNCGAGCTT; GACACCCTAACGAAGACTCTACAAGGACTNCCGCTCATATGCAGNTCCTCGNGACAACTC; CGCTCTGGAGAGCGACANCAGACNANATGGCGGCAAGCACATTAAGCCCAGGGACTGCAC; GC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012479 unedited; GTACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTCAAATCTGTAAGTTTATTTGCTCAATGTACGACAGCTACATAATGACTCACAT; TCATGATATTCCATCACTGAGGAAACTGCTAAAGATGGTCCGTGTGTGAAATAATTCCTT; AAAGAAACACGGAGCTGGAAAAATAATCACTGATTAGACCTTAAAAATAGTTCACTGCAT; AACATGACAAAAAGCACAAAGGCTCATTCAGAGAACATATTTGTTGTTCTCCAACCCTGT; AATAGTTATAATTTCACCATGACAAACACCAGACATTAAGATTAAGCTAACCCTGGTGTT; TCTTTTGCTCCCCCCCTTTTAAAAACAAAATATATAACTGCATGTCACTATAGCAACATC; CAAAACAGATCAATTTGTTACAATCACTATTTGGTAGAGCAAACTTTACCCCCAAAAGGA; AAAATTAAATTAAAAAAAAAAAACCTTTAAAAAATTTGAAGACTTAATTTTTTTTGTCAT; AGGAATACATAACACCTACAGTATAAGTTAATCAATTTCAAGCTACTGTATAGAAATACA; ACACTAGCATGCACAATATTGTATCAATAGAGTAATGGAGGAGTAATCATTTCCCTGGAG; AGACAGTTCATAGGCCAGTGCATTTCTTTAAAAATAAAGAAAAGAAAAACCATTTCAGGC; TGCTTTAATATCAAGTCTCCCATTCCCTCTCCCGTTTCAGCCCCTCAGTGGGGATTTTTA; TCTTGCCTTATTCTAAAAAGCAGCCGGGAGCCAAAGCTGGAATTTTAAGGAAAAATAGTT; TTGTTATTTCAGTAAATCATTTCAAATGCTACAGGGAAAACCCACCTTGCTTCTTGGACT; GAAGATAATATGGGAAAGCCCCCTTCCGTGCGCCCCCCCCCCCCCCCCCNNAAAATAATT; TTTCNTAGGGAAGCTTTGGGGCGGGGAGGAGCCAAAACCGGTGAGTTTTTTTTCTT;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_012479
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012479.2, NP_036611.2
• RefSeq Size: 3747 bp
• RefSeq ORF: 744 bp
• Locus ID: 7532
• UniProt ID: P61981
• Cytogenetics: 7q11.23
• Domains: 14-3-3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
• Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]