APOL3 (NM_145639) Human Untagged Clone

SKU SC124393

APOL3 (untagged)-Human apolipoprotein L, 3 (APOL3), transcript variant alpha/c

Product Data

• Type: Human Untagged Clone
• Target Symbol: APOL3
• Synonyms: apoL-III; APOLIII; CG12_1; CG121
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145639, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGACTCAGAAAAGAAACGCTTTACTGAAGAGGCCACCAAATACTTCCGGGAGAGAGTCAGCCCAGTGC; ATCTGCAAATCCTGCTGACTAACAATGAAGCCTGGAAGAGATTCGTGACTGCGGCTGAATTGCCCAGGGA; TGAGGCAGATGCTCTCTACGAAGCTCTGAAGAAGCTTAGAACATATGCAGCTATTGAGGACGAATATGTG; CAGCAGAAAGATGAGCAGTTTAGGGAATGGTTTTTGAAAGAGTTTCCCCAAGTCAAGAGGAAGATCCAGG; AGTCCATAGAAAAGCTTCGTGCCCTTGCAAATGGTATTGAAGAGGTCCACAGAGGCTGCACCATCTCCAA; TGTGGTGTCCAGCTCCACTGGCGCTGCCTCTGGCATCATGTCCCTTGCTGGTCTTGTTTTGGCACCATTT; ACAGCAGGGACGAGTCTGGCCCTTACTGCAGCTGGGGTAGGGCTGGGAGCAGCGTCTGCTGTGACTGGGA; TCACCACCAGCATCGTGGAGCACTCATACACATCATCAGCAGAAGCTGAAGCCAGCAGGCTGACTGCAAC; CAGCATTGACCGATTGAAGGTATTTAAGGAAGTTATGCGTGACATCACACCCAACTTACTTTCCCTTCTT; AATAATTATTACGAAGCCACACAAACCATTGGGAGTGAAATCCGTGCCATCAGGCAAGCCAGAGCCAGGG; CCCGACTCCCTGTGACCACCTGGCGAATCTCAGCTGGAAGTGGTGGTCAAGCAGAGAGAACGATTGCAGG; CACCACCCGGGCAGTGAGCAGAGGAGCCCGGATCCTGAGTGCGACCACTTCAGGCATCTTCCTTGCACTG; GATGTGGTCAACCTTGTATACGAGTCAAAGCACTTGCATGAGGGGGCAAAGTCTGCATCTGCTGAGGAGC; TGAGGCGGCAGGCTCAGGAGCTGGAGGAGAATCTAATGGAGCTCACTCAGATCTATCAGCGTCTGAATCC; ATGCCATACCCACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145639 unedited; AGGGTTCACTATAGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGGATCCA; CACAGCTCAGAACAGCTGGATCTTGCTCACACTCTTTCAAGAGAAGCTTCCTTGGGTTAA; GAAAAAAAACGAACCCTTCCAGTCAGGTCAGTGACTGGAGAGCTCCAAGGAAAGTCTCTC; AGTGACCTGGCTGCTGGCACCATGGACTCAGAAAAGAAACGCTTTACTGAAGAGGCCACC; AAATACTTCCGGGAGAGAGTCAGCCCAGTGCATCTGCAAATCCTGCTGACTAACAATGAA; GCCTGGAAGAGATTCGTGACTGCGGCTGAATTGCCCAGGGATGAGGCAGATGCTCTCTAC; GAAGCTCTGAAGAAGCTTAGAACATATGCAGCTATTGAGGACGAATATGTGCAGCAGAAA; GATGAGCAGTTTAGGGAATGGTTTTTGAAAGAGTTTCCCCAAGTCAAGAGGAAGATCCAG; GAGTCCATAGAAAAGCTTCGTGCCCTTGCAAATGGTATTGAAGAGGTCCACAGAGGCTGC; ACCATCTCCAATGTGGTGTCCAGCTCCACTGGCGCTGCCTCTGGCATCATGTCCCTTGCT; GGTCTTGTTTTGGCACCATTTACAGCAGGGACGAGTCTGGCCCTTACTGCAGCTGGGGTA; GGGCTGGGAGCAGCGTCTGCTGTGACTGGGATCACCACCAGCATCGTGGAGCACTCATAC; ACATCATCAGCAGAAGCTGAAGCCAGCAGGCTGACTGCAACCAGCATTGACCGATTGAAG; GTATTTTAAGAAGTTATGCGTGACATCACACCCAACTTACTTTCCCTTCTTAATATTATT; ACGAAGCCACACAAACCATTGNGAGTGAAATCCGTGCCATCAGGCAAGCCAGAGCCAGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145639 unedited; NGGGTCCGACTATGNACCCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTG; GTGAGTAGGTGAGTTTATTAGGACTTACACACAGGGCACTCAGCAGGATGGCTCTAGAGA; TCCGGCCTCCCCCAGTCTCTAAACTGCTTTTCAGTTAATTTTCTGTCTCTTTGCCTGCTG; TATATGAGTAATGAGACTGTTTTTCTTGGTAGGTTCTCGCATACTCTCCAGGATGTTTGG; GTTTTTAGAGACACCTGGTCCTCAGCTGGGGACAATGGCCATGGCTCATTACCTGGCCTT; CAGGGTTCAAGCAGGGGACATATACCCCTAAATAACCTAAAGGGGATCCATCACACTACA; ACCACCACCTCCACCGCCATCATCAAGAAGCCACTGGCTGACTGAGATACACTTCCAGGA; GGACAAGACAGAGTGGATGCTGGAAAGACAGGGCAGGGGACCATCACCAGGGAAAGACTT; CATTCTTGGAAGGACATCGAACCGGGGGCAGGTCGGTAGTGGAGCCGCTGTTTCTTCTGC; TGTATCCAAAAGTTCTAACTCTTCGGCTTTCTGCATTTTCAGCTCTTTCTTTTCCTGGCC; TTCTCATTGCTGGTTCCTGCACACCTCCCCTCTATTCCTCCCCCCAATATATTCGTTAGT; CTAAAGGAAATTTCTTCTTCCTATTCCCCACACTCTCCAGTCCCCTCTCCTCCCTTATTC; CAGGCTCCAGCATTCCTGCCTTCTCCTTGGTGCACTTGCCATCTGCATTAACCCCTCCCC; TGCTGTGCTCAGCTACAGAAATGCCAAAGTCACTAACACTCAGCTCCATAAACTTTACCT; TGCCCTCTTTATCCCCCTCATAAATGCCTGCATTTTTGTCTGGCCCGTGTATGGCTCANC; TTCCCTGCTGGCTGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_145639
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145639.1, NP_663614.1
• RefSeq Size: 2215 bp
• RefSeq ORF: 996 bp
• Locus ID: 80833
• UniProt ID: O95236
• Cytogenetics: 22q12.3
• Summary: This gene is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids, including cholesterol, and/or allow the binding of lipids to organelles. In addition, expression of this gene is up-regulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]; Transcript Variant: This variant (alpha/c) differs in the coding region but maintains the reading frame compared to variant alpha/d. This variant encodes isoform 2, which is shorter and which contains a unique N-terminus compared to isoform 1.