ORC6L (ORC6) (NM_014321) Human Untagged Clone

SKU SC124372

ORC6 (untagged)-Human origin recognition complex, subunit 6 (ORC6), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ORC6L
• Synonyms: ORC6L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124372 sequence for NM_014321 edited (data generated by NextGen Sequencing); ATGGGGTCGGAGCTGATCGGGCGCCTAGCCCCGCGCCTGGGCCTCGCCGAGCCCGACATG; CTGAGGAAAGCAGAGGAGTACTTGCGCCTGTCCCGGGTGAAGTGTGTCGGCCTCTCCGCA; CGCACCACGGAGACCAGCAGTGCAGTCATGTGCCTGGACCTTGCAGCTTCCTGGATGAAG; TGCCCCTTGGACAGGGCTTATTTAATTAAACTTTCTGGTTTGAACAAGGAGACATATCAG; AGCTGTCTTAAATCTTTTGAGTGTTTACTGGGCCTGAATTCAAATATTGGAATAAGAGAC; CTAGCTGTACAGTTTAGCTGTATAGAAGCAGTGAACATGGCTTCAAAGATACTAAAAAGC; TATGAGTCCAGTCTTCCCCAGACACAGCAAGTGGATCTTGACTTATCCAGGCCACTTTTC; ACTTCTGCTGCACTGCTTTCAGCATGCAAGATTCTAAAGCTGAAAGTGGATAAAAACAAA; ATGGTAGCCACATCCGGTGTAAAAAAAGCTATATTTGATCGACTGTGTAAACAACTAGAG; AAGATTGGACAGCAGGTCGACAGAGAACCTGGAGATGTAGCTACTCCACCACGGAAGAGA; AAGAAGATAGTGGTTGAAGCCCCAGCAAAGGAAATGGAGAAGGTAGAGGAGATGCCACAT; AAACCACAGAAAGATGAAGATCTGACACAGGATTATGAAGAATGGAAAAGAAAAATTTTG; GAAAATGCTGCCAGTGCTCAAAAGGCTACAGCAGAGTGA; ; Clone variation with respect to NM_014321.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014321 unedited; GGTACAGCATTAGNACACGATTACTATAGGGCGGCCGCGATTCGGCACGAGGATGGGGTC; TGAGCTGATCGGGCGCCTAGCCCCGCGCCTGGGCCTCGCCGAGCCCGACATGCTGAGGAA; AGCAGAGGAGTACTTGCGCCTGTCCCGGGTGAAGTGTGTCGGCCTCTCCGCACGCACCAC; GGAGACCAGCAGTGCAGTCATGTGCCTGGACCTTGCAGCTTCCTGGATGAAGTGTCCCTT; GGACAGGGCTTATTTAATTAAACTTTCTGGTTTGAACAAGGAGACATATCAGAGCTGTCT; TAAATCTTTTGAGTGTTTACTGGGCCTGAATTCAAATATTGGAATAAGAGACCTAGCTGT; ACAGTTTAGCTGTATAGAAGCAGTGAACATGGCTTCAAAGATACTAAAAAGCTATGAGTC; CAGTCTTCCCCAGACACAGCAAGTGGATCTTGACTTATCCAGGCCACTTTTCACTTCTGC; TGCACTGCTTTCAGCATGCAAGATTCTAAAGCTGANAGTGGATAAAAACAAAATGGTAGC; CACATCCGGTGTAAAAAAAGCTATATTTGATCGACTGTGTTAACAACTAGAGAAGATTGG; ACAGCAGGTCGACAGAGAACCTGGAGATGTAGCTACTCCACCCACGGAAAAAAAAGAGAA; TAGTGGTTGAAGCCCCAGCAAGGAAAGGAGAAAGTAGAGGAGATGCCCCTTAACCCCGGA; AGATGAAGATCTGGCCCGGATTATGAAGAATGGAAAAAAAAAATTTTGGAAAAAGCTGCC; CGTGCTCAAAAGCCTCCGCAGAATGATTTTAGCTTCCAAACTGGTTTACTTTCCCAACTG; GAAGTAAATGCGCCTTTCCAGAAAACTTGCCGCTTTGGGATTTTGTTAAAACTTTTATAA; TAAGGATCCAAGAACGTGTGGCCTTTAAAAAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_014321
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014321.2, NP_055136.1
• RefSeq Size: 1647 bp
• RefSeq ORF: 759 bp
• Locus ID: 23594
• UniProt ID: Q9Y5N6
• Cytogenetics: 16q11.2
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• Summary: The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq, Oct 2010]; Transcript Variant: This variant (1) encodes the functional protein.