NUP50 (NM_153645) Human Untagged Clone

SKU SC124316

NUP50 (untagged)-Human nucleoporin 50kDa (NUP50), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: NUP50
• Synonyms: NPAP60; NPAP60L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153645, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCAGTGAGGAAGTCTTGAAGAATAGAGCCATAAAGAAAGCAAAGCGCAGAAATGTTGGATTTGAAT; CTGACACTGGAGGAGCCTTTAAAGGTTTTAAAGGTTTGGTGGTACCTTCTGGAGGAGGACGCTTTTCTGG; ATTTGGTAGTGGCGCTGGAGGGAAGCCTTTGGAAGGACTGTCGAATGGAAACAACATAACCAGTGCCCCT; CCCTTCGCCAGTGCAAAGGCAGCGGCAGATCCCAAGGTAGCCTTTGGTTCTCTTGCTGCAAATGGCCCTA; CCACCTTGGTTGATAAAGTTTCAAATCCCAAAACTAATGGGGACAGTCAGCAGCCCTCCTCCTCTGGCCT; TGCTTCCAGTAAAGCTTGTGTCGGAAATGCCTATCACAAGCAGTTGGCCGCCTTGAACTGCTCCGTGCGG; GATTGGATAGTGAAGCACGTGAATACAAACCCCCTCTGTGATCTGACACCTATCTTTAAAGACTATGAGA; AATATTTAGCAAACATTGAACAGCAACACGGGAACAGTGGCAGGAATTCTGAAAGTGAATCTAACAAAGT; GGCAGCTGAAACACAGTCTCCTTCCCTTTTTGGCTCAACAAAATTACAGCAAGAGTCAACGTTTTTGTTT; CATGGCAACAAAACTGAAGATACACCTGACAAGAAGATGGAGGTGGCATCTGAAAAGAAAACGGACCCAT; CATCACTAGGAGCGACAAGTGCCTCATTTAATTTCGGCAAGAAAGTTGATAGCTCTGTTTTGGGCTCATT; AAGCTCTGTCCCCCTGACTGGATTTTCTTTCTCCCCTGGAAACTCCAGTTTATTTGGCAAAGATACTACC; CAGAGTAAACCAGTCTCTTCACCATTTCCCACTAAACCATTGGAGGGCCAAGCAGAAGGTGACAGTGGTG; AATGCAAAGGTGGAGATGAAGAAGAGAATGATGAGCCACCCAAAGTAGTAGTTACCGAAGTAAAAGAAGA; AGATGCTTTTTACTCCAAAAAGTGTAAACTGTTTTACAAGAAAGACAATGAGTTTAAAGAGAAAGGCATA; GGTACTCTGCATTTAAAACCTACAGCAAATCAGAAGACACAGCTTTTGGTGCGGGCAGACACCAATTTAG; GCAACATATTGCTGAACGTTCTGATTCCACCCAATATGCCATGTACGCGAACAGGGAAGAATAACGTTCT; TATCGTCTGTGTTCCAAATCCACCAATTGACGAGAAGAATGCCACCATGCCAGTCACCATGTTGATTCGG; GTAAAAACCAGCGAGGATGCAGACGAGTTGCACAAAATTTTACTGGAGAAAAAGGATGCCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153645 unedited; ACTCACTATAGGCGGCCGCGNAATTCGCACGAGACGGTTCGAAAACATGGCCAAAAGAAA; GCCGAGAAGGAACTGACAGATAGGAATTGGGATCAAGAAGATGAAGCTGAAGAGGTGGGA; ACATTCTCCATGGCCAGTGAGGAAGTCTTGAAGAATAGAGCCATAAAGAAAGCAAAGCGC; AGAAATGTTGGATTTGAATCTGACACTGGAGGAGCCTTTAAAGGTTTTAAAGGTTTGGTG; GTACCTTCTGGAGGAGGACGCTTTTCTGGATTTGGTAGTGGCGCTGGAGGGAAGCCTTTG; GAAGGACTGTCGAATGGAAACAACATAACCAGTGCCCCTCCCTTCGCCAGTGCAAAGGCA; GCGGCAGATCCCAAGGTAGCCTTTGGTTCTCTTGCTGCAAATGGCCCTACCACCTTGGTT; GATAAAGTTTCAAATCCCAAAACTAATGGGGACAGTCAGCAGCCCTCCTCCTCTGGCCTT; GCTTCCAGTAAAGCTTGTGTCGGAAATGCCTATCACAAGCAGTTGGCCGCCTTGAACTGC; TCCGTGCGGGATTGGATAGTGAAGCACGTGAATACAAACCCCCTCTGTGATCTGACACCT; ATCTTTANAGACTATGAGAAATATTTAGCAAACATTGAACAGCAACACGGGGAACAGTGG; CAGGAATTCTGGAAAGTGAATCTAACAAAGTGGCAGCTGAAACACAGTCTCCTTTCCTTT; TTGGCTCAACAAATTACAGCAGAGTCAACGTTTTTGGTTCATGGCAACAAACTGGAGAAT; CACCTGACAGAAGATGGAGGGGGCTCTGAAAAGAAACGGCCCATCATACTAGGACCGAAC; AGGCCTCATTTAATTCGCAAGAAATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_153645
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153645.1, NP_705931.1
• RefSeq Size: 5051 bp
• RefSeq ORF: 1323 bp
• Locus ID: 10762
• UniProt ID: Q9UKX7
• Cytogenetics: 22q13.31
• Protein Families: Stem cell - Pluripotency
• Summary: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 2. The resulting isoform (a) is shorter at the N-terminus compared to isoform b.