Myostatin Propeptide (MSTN) (NM_005259) Human Untagged Clone

SKU SC124056

MSTN (untagged)-Human myostatin (MSTN)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Myostatin Propeptide
• Synonyms: GDF8; MSLHP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124056 sequence for NM_005259 edited (data generated by NextGen Sequencing); ATGCAAAAACTGCAACTCTGTGTTTATATTTACCTGTTTATGCTGATTGTTGCTGGTCCA; GTGGATCTAAATGAGAACAGTGAGCAAAAAGAAAATGTGGAAAAAGAGGGGCTGTGTAAT; GCATGTACTTGGAGACAAAACACTAAATCTTCAAGAATAGAAGCCATTAAGATACAAATC; CTCAGTAAACTTCGTCTGGAAACAGCTCCTAACATCAGCAAAGATGTTATAAGACAACTT; TTACCCAAAGCTCCTCCACTCCGGGAACTGATTGATCAGTATGATGTCCAGAGGGATGAC; AGCAGCGATGGCTCTTTGGAAGATGACGATTATCACGCTACAACGGAAACAATCATTACC; ATGCCTACAGAGTCTGATTTTCTAATGCAAGTGGATGGAAAACCCAAATGTTGCTTCTTT; AAATTTAGCTCTAAAATACAATACAATAAAGTAGTAAAGGCCCAACTATGGATATATTTG; AGACCCGTCGAGACTCCTACAACAGTGTTTGTGCAAATCCTGAGACTCATCAAACCTATG; AAAGACGGTACAAGGTATACTGGAATCCGATCTCTGAAACTTGACATGAACCCAGGCACT; GGTATTTGGCAGAGCATTGATGTGAAGACAGTGTTGCAAAATTGGCTCAAACAACCTGAA; TCCAACTTAGGCATTGAAATAAAAGCTTTAGATGAGAATGGTCATGATCTTGCTGTAACC; TTCCCAGGACCAGGAGAAGATGGGCTGAATCCGTTTTTAGAGGTCAAGGTAACAGACACA; CCAAAAAGATCCAGAAGGGATTTTGGTCTTGACTGTGATGAGCACTCAACAGAATCACGA; TGCTGTCGTTACCCTCTAACTGTGGATTTTGAAGCTTTTGGATGGGATTGGATTATCGCT; CCTAAAAGATATAAGGCCAATTACTGCTCTGGAGAGTGTGAATTTGTATTTTTACAAAAA; TATCCTCATACTCATCTGGTACACCAAGCAAACCCCAGAGGTTCAGCAGGCCCTTGCTGT; ACTCCCACAAAGATGTCTCCAATTAATATGCTATATTTTAATGGCAAAGAACAAATAATA; TATGGGAAAATTCCAGCGATGGTAGTAGACCGCTGTGGGTGCTCATGA; ; Clone variation with respect to NM_005259.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005259 unedited; GGGCGGCCGCAAATTCGGCACGAGATTCACTGGTGTGGCAAGTTGTCTCTCAGACTGTAC; ATGCATTAAAATTTTGCTTGGCATTACTCAAAAGCAAAAGAAAAGTAAAAGGAAGAAACA; AGAACAAGAAAAAAGATTATATTGATTTTAAAATCATGCAAAAACTGCAACTCTGTGTTT; ATATTTACCTGTTTATGCTGATTGTTGCTGGTCCAGTGGATCTAAATGAGAACAGTGAGC; AAAAAGAAAATGTGGAAAAAGAGGGGCTGTGTAATGCATGTACTTGGAGACAAAACACTA; AATCTTCAAGAATAGAAGCCATTAAGATACAAATCCTCAGTAAACTTCGTCTGGAAACAG; CTCCTAACATCAGCAAAGATGTTATAAGACAACTTTTACCCAAAGCTCCTCCACTCCGGG; AACTGATTGATCAGTATGATGTCCAGAGGGATGACAGCAGCGATGGCTCTTTGGAAGATG; ACGATTATCACGCTACAACGGAAACCATCATTACCATGGCTTCCGAAGTCTGATTTTCTA; ATGCAAGTGGATGGAAAACCCAAATGTTGCTTCTNTTAAATTTAGCTCTANATACAATAC; AATAAAGTAGTAAGGCCCAACTATGGATATATTTGAGAACCGTCGAGACTCCTACAACAG; TGTTTGTGCANATCCTGAGACTCATCANACCTATGAAAGACGGTACAGGTATACTGGAAT; CGAATCTTGAACTTGAATGAACCAGGCACTGTATTTGCAGACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005259 unedited; AGGCACTGGNAAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGAC; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGATAGCATCAGTTTATT; CTTCATTTCAGATAATAGAGTCAATTATTTTGGTATACTTAGTAATGTTTTTGCAAGTAT; TAAAATAATGGAACGTTGAGATTTAAACACAATGACAGTAAACCATTGAAATTTCAAATT; CACTTTATACAGCCATCATGAATCCATAAGTGAATGTTAATCATGTAAAAAAATATAAAA; TGATTGTAATATAACCATCTAATCATTAACATATGGAGTTTTAAGACCACTATTTAATCT; GCTAATTTGCTCTGAAATATAAGTAGTTGCTTTTCTGTGATGCATGACATGTCTTTGTGC; CTTAGTACTTAATTTGAAATGTCCTTAGTGTAAAAATATACCAAAGTAAATAAAAAAGGA; GACACTTATTTACAAAACAATATTGTATACATATTATATAAATGCATTGTTTCATTCTTT; TATACAATATTGATAGAGTCGATCATTTCTATTATTTTACCATAAAAACTGCAGTGTTGC; AAAAGCAGTGTTGTAATTTTCANAGACAAAATACATACTCTCCAGTATGTATTTTGCATT; TTGATAANACAACACCATTGACTGCCTTTAGAAAATGCATTCTTCAATTTGTTACGAATG; AAGCCACCTTTGAAAATGATCTCTTCATAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005259
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005259.1, NP_005250.1
• RefSeq Size: 2823 bp
• RefSeq ORF: 1128 bp
• Locus ID: 2660
• UniProt ID: O14793
• Cytogenetics: 2q32.2
• Protein Families: Druggable Genome, Secreted Protein
• Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016]