SCN4B (NM_174934) Human Untagged Clone

CAT#: SC123982

SCN4B (untagged)-Human sodium channel, voltage-gated, type IV, beta (SCN4B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCN4B
• Synonyms: ATFB17; LQT10; Navbeta4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC324716 sequence for NM_001142349 edited (data generated by NextGen Sequencing)
  
  
  ATGAACAACATTTCCATTGTGCTGAGGGACCTGGAGTTCAGCGACACGGGCAAATACACCTGCCATGTGA
  AGAACCCCAAGGAGAATAATCTCCAGCACCACGCCACCATCTTCCTCCAAGTCGTTGATAGACTGGAAGA
  AGTGGACAACACAGTGACACTCATCATCCTGGCTGTCGTGGGCGGGGTCATCGGGCTCCTCATCCTCATC
  CTGCTGATCAAGAAACTCATCATCTTCATCCTGAAGAAGACTCGGGAGAAGAAGAAGGAGTGTCTCGTGA
  GCTCCTCGGGGAATGACAACACGGAGAACGGCTTGCCTGGCTCCAAGGCAGAGGAGAAACCACCTTCAAA
  AGTGTGA
  
  Clone variation with respect to NM_001142349.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_174934 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCGCAAAGCTTCAGGGTA
  GCTTTGTGGCCGAACGCCGACCTCGGGCGGAGAGCGCGGCTGTGCCCAGTATCCCATCCC
  CGCGACCCCCGCGCGCTCCGGAGAGAACAGGACTATGCCCGGGGCTGGGGACGGAGGCAA
  AGCCCCGGCGAGATGGCTGGGCACTGGGCTTTTGGGCCTCTTCCTGCTCCCCGTAACCCT
  GTCGCTGGAGGTGTCTGTGGGAAAGGCCACCGACATCTACGCTGTCAATGGCACGGAGAT
  CCTGCTGCCCTGCACCTTCTCCAGCTGCTTTGGCTTCGAGGACCTCCACTTCCGGTGGAC
  CTACAACAGCAGTGACGCATTCAAGATTCTCATAGAGGGGACTGTGAAGAATGATAAGTC
  TGACCCCAAGGTGACGTTGAAAGACGATGACCGCATCACTCTGGTAGGCTCTACTAAGGA
  GAAGATGAACAACATTTCCATTGTGCTGAGGGACCTGGAGTTCAGCGACACGGGCAAATA
  CACCTGCCATGTGAAGAACCNCAAGGAGAATAATCTCCAGCACCACGCCACCATCTATCT
  CCAAGTCGTTGATAGACTGGAAGAAGTGGACAACACAGTGACACTCATCAATCCTGGCTG
  TCGTGGGCAGGGTCATCGGGGGCTCTAATCCTCATCCTGCTGATCAAGAAACTTATTAAT
  CTTCATCCTTGAGAATACATCGGAGAAAAAAAAAAGGAGGGCTCTGGGACCTTCTTGGGG
  GAATGACCACCCGGAAAACGCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_174934 unedited
  TATACAGTTTGANGCCAGCAGCAGATGTCTCAGGCACGTGGGTTCTACNGTCNGAGCTCA
  AGCATCAGTTTCATCATCAGAAAGGGGGCTCCCTGCAGCTGCTCAGCCCGAAGCAGGGCT
  CACACTTTTGAAGGTGGTTTCTCCTCTGCCTTGGAGCCAGGCAAGCCGTTCTCCGTGTTG
  TCATTCCCCGAGGAGCTCACGAGACACTCCTTCTTCTTCTCCCGAGTCTTCTTCAGGATG
  AAGATGATGAGTTTCTTGATCAGCAGGATGAGGATGAGGAGCCCGATGACCCCGCCCACG
  ACAGCCAGGATGATGAGTGTCACTGTGTTGTCCACTTCTTCCAGTCTATCAACGACTTGG
  AGGAAGATGGTGGCGTGGTGCTGGAGATTATTCTCCTTGGGGTTCTTCACATGGCAGGTG
  TATTTGCCCGTGTCGCTGAACTCCAGGTCCCTCAGCACAATGGAAATGTTGTTCATCTTC
  TCCTTAGTAGAGCCTACCAGAGTGATGCGGTCATCGTCTTTCAACGTCACCTTGGGGTCA
  GACTTCTCATTCTTCACAGTCCCCTCTATGAGAATCTTGAATGCGTCACTGCTGTTGTAG
  GTCCACCGGAAGTGGAGGTCCTCGAAGCCAAAGCAGCTGGAGAAGGTGCAGGGCAGCAGG
  ATCTCCGTGCCATTGACAGCGTAGATGTCGGTGGCCTTTCCACAGACACCTNCAGCGACA
  GGGTTACCGGGAGCAGGAAGAGGCCCAAAAACCCAGTGCCCACCATCTCGNCNGGGCTTT
  GNNCTCGTCCAGN
  
• Restriction Sites: Please inquire
• ACCN: NM_174934
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174934.1, NP_777594.1
• RefSeq Size: 4489 bp
• RefSeq ORF: 687 bp
• Locus ID: 6330
• UniProt ID: Q8IWT1
• Cytogenetics: 11q23.3
• Protein Families: Ion Channels: Sodium, Transmembrane
• Gene Summary: The protein encoded by this gene is one of several sodium channel beta subunits. These subunits interact with voltage-gated alpha subunits to change sodium channel kinetics. The encoded transmembrane protein forms interchain disulfide bonds with SCN2A. Defects in this gene are a cause of long QT syndrome type 10 (LQT10). Three protein-coding and one non-coding transcript variant have been found for this gene.[provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).