PTGIS (NM_000961) Human Untagged Clone

SKU SC123875

PTGIS (untagged)-Human prostaglandin I2 (prostacyclin) synthase (PTGIS)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PTGIS
• Synonyms: CYP8; CYP8A1; PGIS; PTGI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123875 sequence for NM_000961 edited (data generated by NextGen Sequencing); ATGGCTTGGGCCGCGCTCCTCGGCCTCCTGGCCGCACTGTTGCTGCTGCTGCTACTGAGC; CGCCGCCGCACGCGGCGACCTGGTGAGCCTCCCCTGGACCTGGGCAGCATCCCCTGGTTG; GGGTATGCCTTGGACTTTGGAAAAGATGCTGCCAGCTTCCTCACGAGGATGAAGGAGAAG; CACGGTGACATCTTTACTATACTGGTTGGGGGCAGGTATGTCACCGTTCTCCTGGACCCA; CACTCCTACGACGCGGTGGTGTGGGAGCCTCGCACCAGGCTCGACTTCCATGCCTATGCC; ATCTTCCTCATGGAGAGGATTTTTGATGTGCAGCTTCCACATTACAGCCCCAGTGATGAA; AAGGCCAGGATGAAACTGACTCTTCTCCACAGAGAGCTCCAGGCACTCACAGAAGCCATG; TATACCAACCTCCATGCAGTGCTGTTGGGCGATGCTACAGAAGCAGGCAGTGGCTGGCAC; GAGATGGGTCTCCTCGACTTCTCCTACAGCTTCCTGCTCAGAGCCGGCTACCTGACTCTT; TACGGAATTGAGGCGCTGCCACGCACCCATGAAAGCCAGGCCCAGGACCGCGTCCACTCA; GCTGATGTCTTCCACACCTTTCGCCAGCTCGACCGGCTGCTCCCCAAACTGGCCCGTGGC; TCCCTGTCAGTGGGGGACAAGGACCACATGTGCAGTGTCAAAAGTCGCCTGTGGAAGCTG; CTATCCCCAGCCAGGCTGGCCAGGCGGGCCCACCGGAGCAAATGGCTGGAGAGTTACCTG; CTGCACCTGGAGGAGATGGGTGTGTCAGAGGAGATGCAGGCACGGGCCCTGGTGCTGCAG; CTGTGGGCCACACAGGGGAATATGGGTCCCGCTGCCTTCTGGCTCCTGCTCTTCCTTCTC; AAGAATCCTGAAGCCCTGGCTGCTGTCCGCGGAGAGCTCGAGAGTATCCTTTGGCAAGCG; GAGCAGCCTGTCTCGCAGACGACCACTCTCCCACAGAAGGTTCTAGACAGCACACCTGTG; CTTGATAGCGTGCTGAGTGAGAGCCTCAGGCTTACAGCTGCCCCCTTCATCACCCGCGAG; GTTGTGGTGGACCTGGCCATGCCCATGGCAGACGGGCGAGAATTCAACCTGCGACGTGGT; GACCGCCTCCTCCTCTTCCCCTTCCTGAGCCCCCAGAGAGACCCAGAAATCTACACAGAC; CCAGAGGTATTTAAATACAACCGATTCCTGAACCCTGACGGATCAGAGAAGAAAGACTTT; TACAAGGATGGGAAACGGCTGAAGAATTACAACATGCCCTGGGGGGCGGGGCACAATCAC; TGCCTGGGGAGGAGTTATGCGGTCAACAGCATCAAACAATTTGTGTTCCTTGTGCTGGTG; CACTTGGACTTGGAGCTGATCAACGCAGATGTGGAGATCCCTGAGTTTGACCTCAGCAGG; TACGGCTTCGGTCTGATGCAGCCGGAACACGACGTGCCCGTCCGCTACCGCATCCGCCCA; TGA; ; Clone variation with respect to NM_000961.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000961 unedited; TCAGAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCCCGCCAGC; CCCGCCAGCCCCGCGATGGCTTGGGCCGCGCTCCTCGGCCTCCTGGCCGCACTGTTGCTG; CTGCTGCTACTGAGCCGCCGCCGCACGCGGCGACCTGGTGAGCCTCCCCTGGACCTGGGC; AGCATCCCCTGGTTGGGGTATGCCTTGGACTTTGGAAAAGATGCTGCCAGCTTCCTCACG; AGGATGAAGGAGAAGCACGGTGACATCTTTACTATACTGGTTGGGGGCAGGTATGTCACC; GTTCTCCTGGACCCACACTCCTACGACGCGGTGGTGTGGGAGCCTCGCACCAGGCTCGAC; TTCCATGCCTATGCCATCTTCCTCATGGAGAGGATTTTTGATGTGCAGCTTCCACATTAC; AGCCCCAGTGATGAAAAGGCCAGGATGAAACTGACTCTTCTCCACAGAGAGCTCCAGGCA; CTCACAGAAGCCATGTATACCAACCTCCATGCAGTGCTGTTGGGCGATGCTACAGAAGCA; GGCAGTGGCTGGCACGAGATGGGTCTCCTCGACTTCTCCTACAGCTTCCTGCTCAGAGCC; GGCTACCTGACTCTTTACGGAATTGAGGCGCTGCCACGCACCCATGAAAGCCAGGCCCAG; GACCGCGTCCACTCAGCTGATGTCTTCCACACCTTTCGCCAGCTCGACCGGCTGCTCCCC; AAACTGGCCCGTGGCTCCCTGTCAGTGGGGGACAAGACCACATGTGCAGTGTCAAAAGTC; GCCTGTGGAAGCTGCTATCCCCAGCCAGGCTGCCAGGCGGGCCCACGGAACCAAATGGTG; GAGAAGTACCCGCTGCACCTGGAGGAAATGGGGGTGTCAAAAGAGATGCAAGCACGGGCC; CTGGTGCTGCACCTTGGGAGCACAAGGGGAAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000961 unedited; GCACGTCGTGTTCCGGCTGCATCAGACCGAAGCCGTACCTGCTGAGGTCAAACTCAGGGA; TCTCCACATCTGCGTTGATCAGCTCCAAGTCCAAGTGCACCAGCACAAGGAACACAAATT; GTTTGATGCTGTTGACCGCATAACTCCTCCCCAGGCAGTGATTGTGCCCCGCCCCCCAGG; GCATGTTGTAATTCTTCAGCCGTTTCCCATCCTTGTAAAAGTCTTTCTTCTCTGATCCGT; CAGGGTTCAGGAATCGGTTGTATTTAAATACCTCTGGGTCTGTGTAGATTTCTGGGTCTC; TCTGGGGGCTCAGGAAGGGGAAGAGGAGGAGGCGGTCACCACGTCGCAGGTTGAATTCTC; GCCCGTCTGCCATGGGCATGGCCAGGTCCACCACAACCTCGCGGGTGATGAAGGGGGCAG; CTGTAAGCCTGAGGCTCTCACTCAGCACGCTATCAAGCACAGGTGTGCTGTCTAGAACCT; TCTGTGGGAGAGTGGTCGTCTGCGAGACAGGCTGCTCCGCTTGCCAAAGGATACTCTCGA; GCTCTCCGCGGACAGCAGCCAGGGCTTCAGGATTCTTGAGAAGGAAGAGCAGGAGCCAGA; AAGCAGCGGGACCCATATTCCCCTGTGTGGCCCACAGCTGCAGCACCAGGGCCCGTGCCT; GCATCTCCTCTGACACACCCATCTCCTCCAGGTGCAGCAGGTAACTCTCCAGCCATTTGC; TCCGGTGGGCCCGCCTGGCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_000961
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000961.3, NP_000952.1
• RefSeq Size: 5603 bp
• RefSeq ORF: 1503 bp
• Locus ID: 5740
• UniProt ID: Q16647
• Cytogenetics: 20q13.13
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008]