PHEMX (TSPAN32) (NM_139022) Human Untagged Clone

SKU SC123161

TSPAN32 (untagged)-Human tetraspanin 32 (TSPAN32)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PHEMX
• Synonyms: ART1; PHEMX; PHMX; TSSC6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_139022 edited; GGGGAGGGCGATGTTGACAGACAGACAGAGGGGCGGATGCAGCCTACCTCCTGGGCAGTG; AGCTGCGGTCTGAGGCCCCTGCCCAGCTGGAAACCACAGGGAGGGGAAGGGAGGGGAGGA; GAGGAGAGGAGAGGAACCGTCATGGGGCCTTGGAGTCGAGTCAGGGTTGCCAAATGCCAG; ATGCTGGTCACCTGCTTCTTTATCTTGCTGCTGGGCCTCTCTGTGGCCACCATGGTGACT; CTTACCTACTTCGGGGCCCACTTTGCTGTCATCCGCCGAGCGTCCCTGGAGAAGAACCCG; TACCAGGCTGTGCACCAATGGGCCTTCTCTGCGGGGTTGAGCCTGGTGGGCCTCCTGACT; CTGGGAGCCGTGCTGAGCGCTGCAGCCACCGTGAGGGAGGCCCAGGGCCTCATGGCAGGG; GGCTTCCTGTGCTTCTCCCTGGCGTTCTGTGCACAGGTGCAGGTGGTGTTCTGGAGACTC; CACAGCCCCACCCAGGTGGAGGACGCCATGCTGGACACCTACGACCTGGTATATGAGCAG; GCGATGAAAGGTACGTCCCACGTCCGGCGGCAGGAGCTGGCGGCCATCCAGGACGTGTTT; CTGTGCTGTGGGAAGAAGTCTCCTTTCAGCCGTCTGGGGAGCACAGAGGCTGACCTGTGT; CAGGGAGAGGAGGCGGCGAGAGAGGACTGCCTTCAGGGCATCCGGAGCTTCCTGAGGACA; CACCAGCAGGTCGCCTCCAGCCTGACCAGCATCGGCCTGGCCCTCACGCTTGGACCGCAA; GGGCAAATACACCCTGACCCCACGAGCATGTGGCCGCCAGCCCCAGGAGCCCAGCCTCTT; GAGATGCTCCCAGGGTGGACCCACACATTGTCTCCACTCCGAAGCAGTTGCTATTGGTCC; AAGAGGATGCTCGGGTAGTCTTCGGTGGCTGCAGGAGAGCGATGCTGCGCCTCTGCCCCT; CTCCTGCCACCTGGCTGCCCACAGAGCTCTCCAGGGCAGAAGTCGCGGTGGGCTCAGTGG; GTGCCCTGAGCGGGGTCTCTCAGACTGACGTCAGGCCTTGGTGGGCTGCACTCTCACCTG; GAGGCTCCGGGGAAGCATCTGCCTCCAGGACCATTCAGGCTGTTGACAAGTCAACTCCTC; ATGGCTGTAGGACTGAGGTTCCCAAGTCCTTGTCCCTGGTCCTGTGGTCCCTCCACCTTC; AAACCAGCAATGGTGCATTGAGCAAATTGTGGTCAAATATACATCACATCAAATGTAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139022 unedited; AAAAAGTTAGGATTTGTATACGACTTATATAGGCGGCACGCGAATTCGCCATTACGGCCG; GGGAGGGCGATGTTGACAGACAGACAGAGGGCGGATGCAGCCTACCTCCTGGGCAGTGAG; CTGCGGTCTGAGGCCCCTGCCCAGTCTGGAAACCACAGGGAGGGGAAGGGAGGGGAGGAG; AGGAGAGGAGAGGAACCGTCATGGGGCCTTGGAGTCGAGTCAGGGTTGCCAAATGCCAGA; TGCTGGTCACCTGCTTCTTTATCTTGCTGCTGGGCCTCTCTGTGGCCACCATGGTGACTC; TTACCTACTTCGGGGCCCACTTTGCTGTCATCCGCCGAGCGTCCCTGGAGAAGAACCCGT; ACCAGGCTGTGCACCAATGGGCCTTCTCTGCGGGGTTGAGCCTGGTGGGCCTCCTGACTC; TGGGAGCCGTGCTGAGCGCTGCAGCCACCGTGAGGGAGGCCCAGGGCCTCATGGCAGGGG; GCTTCCTGTGCTTCTCCCTGGCGTTCTGTGCACAGGTGCAGGTGGTGTTCTGGAGACTCC; ACAGCCCCACCCAGGTGGAGGACGCCATGCTGGACACCTACGACCTGGTATATGAGCAGG; CGATGAAAGGTACGTCCCACGTCCGGCGGCAGGAGCTGGCGGCCATCCANGACGTGTTTC; TGTGCTGTGGGAAGAAGTCTCCTTTCAGCCGTCTGGGGAGCACAGAGGCTGACCTGTGTC; AGGGAGAGGATGCGGCGAGAGAAGACTGCCTNTCAGGCATCCGGAGCTTCCTGAGGACCA; CCAGCAGGTCGCCTCCAGCCTGACCAGCATCGGCCTGGCCCTCACGCTTGGGACCGCAGG; GGCAAATACACCCTGACCCCACGAGCATGTGGCCCGCCAGCCCCA;
• Restriction Sites: Please inquire
• ACCN: NM_139022
• Insert Size: 1286 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139022.2, NP_620591.3
• RefSeq Size: 1376 bp
• RefSeq ORF: 963 bp
• Locus ID: 10077
• UniProt ID: Q96QS1
• Cytogenetics: 11p15.5
• Protein Families: Transmembrane
• Summary: This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]