TWIST2 (NM_057179) Human Untagged Clone

SKU SC123104

TWIST2 (untagged)-Human twist homolog 2 (Drosophila) (TWIST2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TWIST2
• Synonyms: AMS; BBRSAY; bHLHa39; DERMO1; FFDD3; SETLSS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_057179 edited; CTAGAGTTTCCAAAAAAGTTAGAATAACTTCCTCTCCCGGAGACCTCGGTTTTGCACAAG; CCGGCCTTGAAATCAGAGCCTTTCCAGCAACTCCGAGAGCGTGTGCTCGGCGACCGCGGG; CTTGGCCAGCGGCGCGCGCTCGGCGCCCCGGCGCCCCCAGCCCCACGCGCGCCGGGCGGG; CGCCATGGAGGAGGGCTCCAGCTCGCCCGTGTCCCCCGTGGACAGCCTGGGCACCAGCGA; GGAGGAGCTCGAGAGGCAGCCCAAGCGCTTCGGCCGGAAGCGGCGCTACAGCAAGAAGTC; GAGCGAAGATGGCAGCCCGACCCCGGGCAAGCGCGGCAAGAAGGGCAGCCCCAGCGCGCA; GTCCTTCGAGGAGCTGCAGAGCCAGCGCATCCTGGCCAACGTGCGCGAGCGCCAGCGCAC; CCAGTCGCTCAACGAGGCCTTCGCGGCGCTGCGCAAGATCATCCCCACGCTGCCCTCTGA; CAAGCTGAGCAAGATCCAGACGCTCAAGCTGGCCGCCAGGTACATAGACTTCCTCTACCA; GGTCCTGCAGAGCGACGAGATGGACAATAAGATGACCAGCTGCAGCTACGTGGCCCACGA; GCGCCTCAGCTACGCCTTCTCCGTGTGGCGCATGGAGGGCGCGTGGTCCATGTCCGCCTC; CCACTAGCGCCGCGCCACCCACCTCCGGACCGGCGCGCCAGGAGGAGATTCTGAATGATG; CTGCCATTGCCAGACCTTCTGCTACCAAACAGAGCGAAGAGGAAGAGGGGGCAGTGTTTG; GTCAAGACAGCCCACGAAACGTTGGCGGGAGTGAGCACATTAGCAATGGCTAAGAACATG; CTTGAATCATTTGTAACAACCCAGGACAGAGATGCCACAAGAGGACTTTGGGGAAAGGCG; AATCCACTCATACATCTTGGAGTTGTTTTTTCAAGTTCAGAAAATGAACCTTCACCAAGG; TCTTGGAACATGAAGCAGAGACTCTGGTGCTGACTCTATCATTTGAGCTCCTAAGGGTAC; TCATGCCCAGTGGAGCATGCACACCAGCAACACCGCCAACACCATCTCGGTGTCCCACCC; CTTAGAGGAGGAAGAGGACACAGAGGGGCGCCCGGTAATAACCCTCATTTCCTCTCTTCC; ACCCCACCACATCATCTTAATAAAACATGAAGGTAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057179 unedited; TTAAACCCTACAGAATTTGTATACTACTCATATAGGCGGCACGCGAATTCGCACGAGGCT; AGAGTTTCCAAAAAATTAGAATAACTTCCTCTCCCGGAGACCTCGGTTTTGCACAAGCCG; GCCTTGAAATCAGAGCCTTTCCAGCAACTCCGAGAGCGTGTGCTCGGCGACCGCGGGCTT; GGCCAGCGGCGCGCGCTCGGCGCCCCGGCGCCCCCAGCCCCACGCGCGCCGGGCGGGCGC; CATGGAGGAGGGCTCCAGCTCGCCCGTGTCCCCCGTGGACAGCCTGGGCACCAGCGAGGA; GGAGCTCGAGAGGCAGCCCAAGCGCTTCGGCCGGAAGCGGCGCTACAGCAAGAAGTCGAG; CGAAGATGGCAGCCCGACCCCGGGCAAGCGCGGCAAGAAGGGCAGCCCCAGCGCGCAGTC; CTTCGAGGAGCTGCAGAGCCAGCGCATCCTGGCCAACGTGCGCGAGCGCCAGCGCACCCA; GTCGCTCAACGAGGCCTTCGCGGCGCTGCGCAAGATCATCCCCACGCTGCCCTCTGACAA; GCTGAGCAAGATCCAGACGCTCAAGCTGGCCGCCAGGTACATAGACTTCCTCTACCAGGT; CCTGCATAGCGACGAGATGGACAATAAGATGACCAGCTGCAGCTACGTGGCCCACGAGCG; CCTCAGCTACGCCTTCTCCGTGTGGCGCATGGAGGGCGCGTGGTCCATGTCCGCCTCCCA; CTAGCGCCGCGCCACCCACCTCCGGACCGGCGCGCCAGGAGGAGATTCTGAATGATGCTG; CCATTGCCAGACCTTCTGCTACCAAACAGATCGAANAGGAAGAGGGGGCAGTGTTTGGT;
• Restriction Sites: Please inquire
• ACCN: NM_057179
• Insert Size: 1192 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_057179.1, NP_476527.1
• RefSeq Size: 1381 bp
• RefSeq ORF: 483 bp
• Locus ID: 117581
• UniProt ID: Q8WVJ9
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome, Transcription Factors
• Summary: The protein encoded by this gene is a basic helix-loop-helix type transcription factor and shares similarity with Twist. This protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype during osteoblast development. This gene may be upregulated in certain cancers. Mutations in this gene cause focal facial dermal dysplasia 3, Setleis type. Two transcript variants encoding the same protein have been found. [provided by RefSeq, Apr 2014]; Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.