EFCAB4B (CRACR2A) (NM_032680) Human Untagged Clone

SKU SC123049

EFCAB4B (untagged)-Human EF-hand calcium binding domain 4B (EFCAB4B), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: EFCAB4B
• Synonyms: EFCAB4B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032680 edited; GTCGGGCGGAGCAGGCGGCTGCTTGACAGCTGAGAGCCGAGCGGGCGGCGCGGGCAGGTC; CACGGGAAGCCTACACGCCCAAGGTCATGCCCTGACCACAGCTGCCCTTCAGGAGGCTGG; CACGGAGCCACCTGGGGCCATTGGGCTGTTTGTCAAGGGTGGGGCAGCAGCAACTTGCCA; CTCTTCCTCCATGAGCCCAGCCAGAGCCTCCACTGGGCACTTAGCACAAGAAAAACCCTG; CAGGAGCCGGATCCAGAGCGCTGGGATTCAGCAGGAGTGCCAGGAGAATTCAACACAGGT; GTGCTGCCTCCTCTCCTGCCAGGTGTGTGGCTGCACAGGAGTCTTGCTGATGGTGGAATT; CAAGTCACCAGTGGCCAAAGTACCCTCCAGGTGTGAAGAGTTGCAGGTCCTCCGAGGAGC; CGGGAGCTGCAAGGTTCTGAAAAAGACTTCAAGAAACTGACTAATCGCGATGGCTGCCCC; TGACGGGAGGGTAGTCTCCAGACCCCAGAGACTTGGTCAGGGGTCTGGCCAGGGGCCAAA; GGGGAGTGGAGCCTGCCTGCATCCCCTGGACAGCCTGGAGCAGAAGGAGACTCAGGAGCA; AACGTCGGGCCAGCTAGTCATGCTGAGGAAGGCACAGGAGTTCTTTCAGACCTGTGATGC; TGAAGGCAAGGGCTTCATCGCCAGGAAGGATATGCAGAGGCTGCATAAGGAGCTACCGCT; CAGCCTGGAGGAACTGGAGGATGTGTTTGATGCCCTGGATGCTGATGGCAATGGCTATCT; GACCCCACAGGAGTTCACTACTGGATTTAGTCACTTCTTCTTCAGCCAGAATAACCCAAG; TCAGGAAGATGCAGGTGAACAGGTGGCCCAGCGCCATGAAGAGAAGGTGTATCTGTCCAG; AGGGGATGAGGATCTGGGCGACATGGGCGAAGATGAGGAAGCCCAGTTCCGGATGCTGAT; GGACAGACTTGGAGCCCAAAAGGTGTTGGAAGATGAAAGTGATGTCAAGCAGCTCTGGTT; GCAGCTGAAGAAGGAGGAACCTCATTTACTGTCCAACTTTGAAGACTTCCTGACCAGAAT; CATCTCCCAGCTCCAAGAAGCCCATGAGGAGAAGAATGAACTGGAGTGTGCCCTAAAAAG; GAAAATTGCTGCTTATGATGAAGAAATCCAGCATCTCTATGAGGAGATGGAACAACAAAT; CAAAAGTGAGAAGGAGCAGTTTCTCCTGAAGGACACAGAGAGGTTTCAAGCCCGCAGTCA; AGAGCTGGAGCAGAAACTGTTATGTAAGGAGCAGGAGCTGGAGCAGCTCACCCAGAAGCA; GAAAAGGCTGGAAGGTCAGTGCACAGCCCTGCATCATGACAAGCATGAGACCAAGGCTGA; GAATACCAAGCTGAAACTCACTAACCAGGAGCTGGCCCGGGAGCTGGAGCGGACTTCCTG; GGAGCTCCAGGATGCTCAGCAGCAGTTGGAAAGCCTCCAGCAAGAGGCCTGCAAACTCCA; CCAAGAGAAGGAGATGGAAGTGTACCGTGTGACGGAGAGTCTACAGCGTGAGAAGGCCGG; GCTCCTCAAGCAGCTGGATTTCCTAAGGTGCGTCGGTGGGCACTGGCCTGTGCTGAGAGC; ACCTCCCAGAAGCCTGGGGTCGGAAGGACCAGTCTGAGGGTCATCCTTGCCTACGTGCTG; GAAAAACGTGCCTGCTGAGAGAGTGCCCTCAATTCCCTTGGTCCCCAGTGCAGTGTTTGG; ACCACCAAGTAGCCACTCTATTATGTTTGGTCCGTTTACACATGCGCCCACATCCAGTTT; ATTCCTCCTGGTGGGGAGGCTCTGCCCCACTCCTCTGAGTCCCTCACAGGACTGTGCTTG; GTACTTGATCTCATGCGAGCAGAGCTGGGTTTAATGAGCATTTAATCTATGCTAGAAATA; TTATCTCGTTCATTGTCAGCAAAACTGTTAGGTGAGTAGGGCAGTTCATTCACAGGAATT; TACTGATGGGAAGTTGATATTAGAGGAAAGAAGTAATTTACCACAGGCGACACAGCTACT; CTCTGATGGGGCCAGAATGGAAATCCGTAACTCGGCTGTGTTCTGTTCATGTTTGTTGAA; TGAGTAAATGAATCACAAACAGAGCACCAAGAAAAAGCTTTCTCAGCCTTCAAAGACCCC; CAGCAATAAACTCAAAGTTTACTGGTAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032680 unedited; ACGTTCAAAATTTGTAATACGACTCACTATAGNNGGCGGCCGCGAATCGGCACGAGGGTC; GGGCGGAGCAGGCGGCTGCTTGACAGCTGAGAGCCGAGCGGGCGGCGCGGGCAGGTCCAC; GGGAAGCCTACACGCCCAAGGTCATGCCCTGACCACAGCTGCCCTTCAGGAGGCTGGCAC; GGAGCCACCTGGGGCCATTGGGCTGTTTGTCAAGGGTGGGGCAGCAGCAACTTGCCACTC; TTCCTCCATGAGCCCAGCCAGAGCCTCCACTGGGCACTTAGCACAAGAAAAACCCTGCAG; GAGCCGGATCCAGAGCGCTGGGATTCAGCAGGAGTGCCAGGAGAATTCAACACAGGTGTG; CTGCCTCCTCTCCTGCCAGGTGTGTGGCTGCACAGGAGTCTTGCTGATGGTGGAATTCAA; GTCACCAGTGGCCAAAGTACCCTCCAGGTGTGAAGAGTTGCAGGTCCTCCGAGGAGCCGG; GAGCTGCAAGGTTCTGAAAAAGACTTCAAGAAACTGACTAATCGCGATGGCTGCCCCTGA; CGGGAGGGTAGTCTCCAGACCCCAGAGACTTGGTCAGGGGTCTGGCCAGGGGCCAAAGGG; GAGTGGAGCCTGCCTGCATCCCCTGGACAGCCTGGAGCAGAAGGAGACTCAGGAGCAAAC; GTCGGGCCAGCTAGTCATGCTGAGGAAGGCACAGGAGTTCTTTCAGACCTGTGATGCTGA; AGGCAAGGGCTTCATCGCCAGGAAGGATATGCAGAGGCTGCATAAGGAGCTACCGCTCAG; CCTGGNAGAACTGGGAGATGTGTTTGATGCCCTGGATGCTGATGGCAATGGCTATCTGAC; CCACAGGAGTTCACTACTGGATTTAGTCACTTCTTNCTCAGCCAGAATAACCCAGTCAGG; AAGATGCANGTGAACAGGTGA;
• Restriction Sites: Please inquire
• ACCN: NM_032680
• Insert Size: 2213 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032680.2, NP_116069.1
• RefSeq Size: 2204 bp
• RefSeq ORF: 1188 bp
• Locus ID: 84766
• UniProt ID: Q9BSW2
• Cytogenetics: 12p13.32
• Summary: Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a shorter and distinct C-terminus, compared to isoform a.