Lin28 (LIN28A) (NM_024674) Human Untagged Clone

CAT#: SC122965

LIN28A (untagged)-Human lin-28 homolog A (LIN28A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Lin28
• Synonyms: CSDD1; LIN-28; lin-28A; LIN28; ZCCHC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024674 edited
  GTGCGGGGGAAGATGTAGCAGCTTCTTCTCCGAACCAACCCTTTGCCTTCGGACTTCTCC
  GGGGCCAGCAGCCGCCCGACCAGGGGCCCGGGGCCACGGGCTCAGCCGACGACCATGGGC
  TCCGTGTCCAACCAGCAGTTTGCAGGTGGCTGCGCCAAGGCGGCAGAAGAGGCGCCCGAG
  GAGGCGCCGGAGGACGCGGCCCGGGCGGCGGACGAGCCTCAGCTGCTGCACGGTGCGGGC
  ATCTGTAAGTGGTTCAACGTGCGCATGGGGTTCGGCTTCCTGTCCATGACCGCCCGCGCC
  GGGGTCGCGCTCGACCCCCCAGTGGATGTCTTTGTGCACCAGAGTAAGCTGCACATGGAA
  GGGTTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAG
  GGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGG
  CGGCCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGT
  GGAGGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCAC
  TTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGGGCCCT
  AGTGCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACC
  CTGCTCCCGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTTTTGCTATCAGG
  AAGTTTCGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTAGTTGG
  CACTGCCATGTATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGG
  GGAAAGGGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAGTGAGGGTTCTG
  GGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATCCCCAG
  AATTTCCAGCTTTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATA
  TATAATAATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATTCATGGAGCCAA
  GCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCACATCT
  TGTATCCTCATACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAAT
  GATGATGGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTCTATGCTCACCC
  CCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCACCTTGG
  GTCCCACTTTCTCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCA
  TTTTAACTTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTATATTTTAATCT
  AAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAGCCAGC
  AGCAGCTCCAGGTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGC
  AACTATTTGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAAGAGCTGGCTTT
  TCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGACCAGG
  TTTTAACTGGGTGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTA
  AACAGGAAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAAAAGGCCAGAGA
  GCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTACATCTC
  CAGATCTGTTCTTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGT
  TTGTAAAACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGTACTTAATCCTG
  TGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATTTTCGG
  GTTCCAGGGAGACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGC
  TACAGTAGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGTTTCGTTTACAC
  GCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATAGAACG
  CATTGGGTGGTGTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGT
  TACTGTATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGGTGGCCTAGTTT
  GTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAAACTTC
  TTTTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAA
  AAAGATCTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATCTTGAACCATCC
  TTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAAAAACT
  TGTCTTCTACCCTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACA
  CATACACATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTATGCAAAAATACT
  GAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTACCATT
  TCTTTCTTTCTTTTTTTTTTTTTTTAAAATGGAGTCTCGCTGTGTCGCCCAGGCTGGAGT
  GCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGACTCTGTCTC
  AAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTA
  ATTTTAATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTG
  GGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGGAACTTTTAT
  GCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGA
  ATGTAACTCCATGTTTACTGCTAGAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGG
  GCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTCCCTCATTCC
  TGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTA
  TTTGATGGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAA
  CACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCTGGAAAAATACAATGAATAAATAAA
  GACTTATTGGTACTCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024674 unedited
  NNNNCCCAGTACACAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGG
  TGCGGGGGAAGAGTAGCAGCTTCTTCTCCGAACCAACCCTTTGCCTTCGGACTTCTCCGG
  GGCCAGCAGCCGCCCGANAGGGGCCCGGGGCCACGGGCTCAGCCGACGACCATGGGCTCC
  GTGTCCAACCAGCAGTTTGCAGGTGGCTGCGCCAAGGCGGCAGAAGAGGCGCCCGAGGAG
  GCGCCGGAGGACGCGGCCCGGGCGGCGGACGAGCCTCAGCTGCTGCACGGTGCGGGCATC
  TGTAAGTGGTTCAACGTGCGCATGGGGTTCGGCTTCCTGTCCATGACCGCCCGCGCCGGG
  GTCGCGCTCGACCCCCCAGTGGATGTCTTTGTGCACCAGAGTAAGCTGCACATGGAAGGG
  TTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAGGGT
  CTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGGCGG
  CCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGA
  GGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCACTTC
  TGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAAGCCCAGCAGGGCCCTAGT
  GCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAANAAGAAATCCACAGCCCTACCCT
  GCTCCCGGAGGCACAGAATTGAGCCACATGGGTGGGGGCTATTCTTTTGCTATCAGGAAG
  TTTCGAGGACAGGCAGAGN
  
• Restriction Sites: Please inquire
• ACCN: NM_024674
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024674.3, NP_078950.1
• RefSeq Size: 3513 bp
• RefSeq ORF: 630 bp
• Locus ID: 79727
• UniProt ID: Q9H9Z2
• Cytogenetics: 1p36.11
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. [provided by RefSeq, Sep 2015]