NXF3 (NM_022052) Human Untagged Clone

SKU SC122921

NXF3 (untagged)-Human nuclear RNA export factor 3 (NXF3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NXF3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022052 edited; CCAAACTCCCGTCCCCAGGCCACACAGATTCTCCTCAAGAGAGCCCTATAAGGACATTGG; TAAAATGTCACTGCCTTCAGGACACACTACGGGTCACACTGATCAAGTTGTTCAAAGAAG; AGCAAGATGTTGGGATATTTACCAAAGGAGATTTAGCAGTAGGTCTGAACCTGTCAATCC; TGGCATGCATTCTTCATCCCATCAGCAGCAAGATGGAGATGCAGCAATGCATGGTGCCCA; CATGGACTCTCCAGTAAGATACACTCCCTATACTATTTCACCCTATAATCGGAAAGGCAG; TTTTCGTAAACAAGACCAAACCCACGTTAACATGGAGAGAGAGCAAAAACCTCCAGAGAG; AAGAATGGAGGGGAACATGCCGGATGGGACCTTAGGGAGCTGGTTCAAGATCACAGTTCC; CTTTGGCATAAAATACAATGAGAAGTGGCTGCTGAATTTGATTCAGAATGAATGCAGTGT; ACCCTTCGTCCCAGTTGAGTTTCACTATGAAAACATGCATGCCAGCTTCTTTGTGGAGAA; TGCCAGCATCGCCTATGCACTGAAGAATGTCAGTGGCAAGATTTGGGATGAGGATAATGA; AAAGATATCAATCTTTGTCAACCCTGCTGGCATACCCCACTTTGTGCACAGGGAGCTGAA; GTCAGAAAAGGTGGAGCAGATAAAGCTGGCCATGAACCAACAGTGTGATGTCTCCCAGGA; AGCTCTTGATATCCAGAGACTCCCATTTTACCCAGACATGGTGAACCGTGATACTAAGAT; GGCATCGAATCCTAGAAAGTGCATGGCTGCCTCCCTGGACGTCCATGAAGAGAACATACC; TACAGTGATGTCTGCAGGGGAGATGGACAAGTGGAAAGGGATAGAGCCAGGAGAGAAGTG; TGCGGACAGAAGCCCAGTGTGCACGACCTTCTCGGATACCTCCAGCAACATAAACTCCAT; CCTGGAATTGTTCCCCAAATTATTATGCCTGGATGGCCAGCAGTCACCCAGAGCAACTTT; ATGTGGTACTGAAGCCCACAAGAGGTTACCAACCTGTAAGGGAAGCTTCTTTGGATCTGA; GATGTTGAAGAATCTGGTCCTGCAATTCCTGCAGCAGTATTACTTGATCTATGACTCTGG; AGATCGACAGGGTCTCCTTAGTGCTTACCACGATGAGGCCTGCTTCTCCCTGAGCATTCC; CTTCAATCCTGAGGACTCAGCCCCGAGCAGCTTCTGCAAGTTCTTCAAGGATAGCAGGAA; TATAAAAATTCTCAAGGACCCCTACCTTCGGGGGGAGCTGCTGAAGCACACAAAACTTGA; TATTGTGGACTCCCTCAGTGCGTTGCCTAAAACTCAGCATGACCTCAGCTCCTTCCTGGT; GGACATGTGGTACCAGACGGAATGGATGCTCTGCTTTTCTGTCAACGGGGTGTTCAAGGA; AGTGGAAGGACAGTCTCAGGGTTCTGTTCTCGCCTTCACCCGGACCTTCATTGCTACCCC; TGGCAGCAGTTCCAGTCTGTGCATCGTGAATGACAAGCTCTTTGTGCGGGATACCAGCCA; CCAAGGGACCCAGAGTGCCTTGTTCACCCTAGTGCCCACAGCCTTCTCCAGCTCCGTGCC; TGCCTTCTCCCAGGAGCAGCAGAAAATGCTGCCTTCGTAACAACCAGTGTGACTACGTCA; GAGCTCCTCATGTTCTTGCATGGCCCAAGATGTATTTCCCTGTGACTGAACCAGGAGATC; TGGCTGGGGCTAAGAAGCCGAAGCCCAACTCACAGGGCTGACTCTCATCCTTCCAAAGGG; GCCAGTTGTTCTGTGCACTTGTCCACCCCTGTTACCCCCAGGATCTCTGTTCCTTTTCAC; AATAAAGTATGGTGTCTTGTTATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022052 unedited; GAGTTTTGAATTTGTAATACGACTTATTATAGGGCGGCCGCGACTTCCCGGGATCCAAAC; TCCCGTCCCCAGGCCACACAGATTCTCCTCAAGAGAGCCCTATAAGGACATTGGTAAAAT; GTCACTGCCTTCAGGACACACTACGGGTCACACTGATCAAGTTGTTCAAAGAAGAGCAAG; ATGTTGGGATATTTACCAAAGGAGATTTAGCAGTAGGTCTGAACCTGTCAATCCTGGCAT; GCATTCTTCATCCCATCAGCAGCAAGATGGAGATGCAGCAATGCATGGTGCCCACATGGA; CTCTCCAGTAAGATACACTCCCTATACTATTTCACCCTATAATCGGAAAGGCAGTTTTCG; TAAACAAGACCAAACCCACGTTAACATGGAGAGAGAGCAAAAACCTCCAGAGAGAAGAAT; GGAGGGGAACATGCCGGATGGGACCTTAGGGAGCTGGTTCAAGATCACAGTTCCCTTTGG; CATAAAATACAATGAGAAGTGGCTGCTGAATTTGATTCAGAATGAATGCAGTGTACCCTT; CGTCCCAGTTGAGTTTCACTATGAAAACATGCATGCCAGCTTCTTTGTGGAGAATGCCAG; CATCGCCTATGCACTGAAGAATGTCAGTGGCAAGATTTGGGATGAGGATAATGAAAAGAT; ATCAATCTTTGTCAACCCTGCTGGCATACCCCACTTTGTGCACAGGGAGCTGAAGTCAGA; AAAGGTGGAGCAGATAAAGCTGGCCATGAACCAACAGTGTGATGTCTCCCAGGAAGCTCT; TGATATCCAGAGACTCCCATTTTACCCAGACATGGTGAACCGTGATACTAAGATGGCATC; GAATCCTAGAAAGTGCATGGCTGCCTCCCTGGACGTCCATGAAGAGAACATACCTACAGT; GATGTCTGCAGGGGAGATGGACAAGTGGAAAGGGATAGAC;
• Restriction Sites: Please inquire
• ACCN: NM_022052
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022052.1, NP_071335.1
• RefSeq Size: 1915 bp
• RefSeq ORF: 1596 bp
• Locus ID: 56000
• UniProt ID: Q9H4D5
• Cytogenetics: Xq22.1
• Summary: This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq, Jul 2008]