DHH (NM_021044) Human Untagged Clone

SKU SC122903

DHH (untagged)-Human desert hedgehog (DHH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DHH
• Synonyms: GDMN; GDXYM; HHG-3; SRXY7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021044 edited; GTCCGGAGTCCGGGCGTTCTGGCAGCACCAGAGTCCTGCAGCAGAGACGGGCTGGGCTGG; CACTCACCCACGACCCCCCTTCCCTGCTATGATGGTTTGTCAGTAGCAGGTCCTAGACAC; CCCCCGCCCGCTCCCGGGGCACGTGGGCAGAGCTAGCAGCAGCCAGGTGCACGGAGCCAC; AAGAGCTCTAGGGCACTCTGGGGGCAGCTGTGCCTGCTGCCCTCTCTGGTGCCTGTGGAG; ATGCCTAACTGACAGGAGAGTTAGTGAGGACAAGAACGCTCCCTTTTGGCCGAGGTCCGC; TGTATCCATGGCTCTCCTGACCAATCTACTGCCCCTGTGCTGCTTGGCACTTCTGGCGCT; GCCAGCCCAGAGCTGCGGGCCGGGCCGGGGGCCGGTTGGCCGGCGCCGCTATGCGCGCAA; GCAGCTCGTGCCGCTACTCTACAAGCAATTTGTGCCCGGCGTGCCAGAGCGGACCCTGGG; CGCCAGTGGGCCAGCGGAGGGGAGGGTGGCAAGGGGCTCCGAGCGCTTCCGGGACCTCGT; GCCCAACTACAACCCCGACATCATCTTCAAGGATGAGGAGAACAGTGGAGCCGACCGCCT; GATGACCGAGCGTTGTAAGGAGCGGGTGAACGCTTTGGCCATTGCCGTGATGAACATGTG; GCCCGGAGTGCGCCTACGAGTGACTGAGGGCTGGGACGAGGACGGCCACCACGCTCAGGA; TTCACTCCACTACGAAGGCCGTGCTTTGGACATCACTACGTCTGACCGCGACCGCAACAA; GTATGGGTTGCTGGCGCGCCTCGCAGTGGAAGCCGGCTTCGACTGGGTCTACTACGAGTC; CCGCAACCACGTCCACGTGTCGGTCAAAGCTGATAACTCACTGGCGGTCCGGGCGGGCGG; CTGCTTTCCGGGAAATGCAACTGTGCGCCTGTGGAGCGGCGAGCGGAAAGGGCTGCGGGA; ACTGCACCGCGGAGACTGGGTTTTGGCGGCCGATGCGTCAGGCCGGGTGGTGCCCACGCC; GGTGCTGCTCTTCCTGGACCGGGACTTGCAGCGCCGGGCTTCATTTGTGGCTGTGGAGAC; CGAGTGGCCTCCACGCAAACTGTTGCTCACGCCCTGGCACCTGGTGTTTGCCGCTCGAGG; GCCGGCGCCCGCGCCAGGCGACTTTGCACCGGTGTTCGCGCGCCGGCTACGCGCTGGGGA; CTCGGTGCTGGCGCCCGGCGGGGATGCGCTTCGGCCAGCGCGCGTGGCCCGTGTGGCGCG; GGAGGAAGCCGTGGGCGTGTTCGCGCCGCTCACCGCGCACGGGACGCTGCTGGTGAACGA; TGTCCTGGCCTCTTGCTACGCGGTTCTGGAGAGTCACCAGTGGGCGCACCGCGCTTTTGC; CCCCTTGAGACTGCTGCACGCGCTAGGGGCGCTGCTCCCCGGCGGGGCCGTCCAGCCGAC; TGGCATGCATTGGTACTCTCGGCTCCTCTACCGCTTAGCGGAGGAGCTACTGGGCTGAGC; GTCCCAGGCATAGAAACCTCGAAGCGCCCGAGGAAACGAGGGCCTGCTGGCTGAGATATG; GGGCTGTGGGCAGCAGACGATGCCGACTGGAAGGGAGGGAGAGGGAGGGGGAGGGAGAAA; ATGGGGCTATGCCTGGCTTAGGGGCAACCTCGTACTGAGAGGAGGTGATACCGGGTCCTA; GGTAGGTAGGGCTGATGGTGCGCACTCCTTAAAGAGGACTATTTAGCCTCTCCCCCAGTG; CTTCAGCCCCACCCGATAATTTTATTTTATTTCTTATTTATAAATTGTAATATAATGATT; CCCTTGCCCAGCTTGCCAAGGTGAGGGGCTGGGGCATGGCATTAACACTGTCTGACACAG; CCAGCCAGTCTGACATCTGACGTTTTCCTGCAAGGTGGGCTAATAAAGGGAAGGCTTTCA; GGAGCTTACTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021044 unedited; GTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATCCCGGGNTGTCCGGAGTCCGG; GCGTTCTGGCAGCACCAGAGTCCTGCAGCAGAGACGGGCTGGGCTGGCACTCACCCACGA; CCCCCCTTCCCTGCTATGATGGTTTGTCAGTAGCAGGTCCTAGACACCCCCCGCCCGCTC; CCGGGGCACGTGGGCAGAGCTAGCAGCAGCCAGGTGCACGGAGCCACAAGAGCTCTAGGG; CACTCTGGGGGCAGCTGTGCCTGCTGCCCTCTCTGGTGCCTGTGGAGATGCCTAACTGAC; AGGAGAGTTAGTGAGGACAAGAACGCTCCCTTTTGGCCGAGGTCCGCTGTATCCATGGCT; CTCCTGACCAATCTACTGCCCCTGTGCTGCTTGGCACTTCTGGCGCTGCCAGCCCAGAGC; TGCGGGCCGGGCCGGGGGCCGGTTGGCCGGCGCCGCTATGCGCGCAAGCAGCTCGTGCCG; CTACTCTACAAGCAATTTGTGCCCGGCGTGCCAGAGCGGACCCTGGGCGCCAGTGGGCCA; GCGGAGGGGAGGGTGGCAAGGGGCTCCGAGCGCTTCCGGGACCTCGTGCCCAACTACAAC; CCCGACATCATCTTCAAGGATGAGGAGAACAGTGGAGCCGACCGCCTGATGACCGAGCGT; TGTAAGGAGCGGGTGAACGCTTTGGCCATTGCCGTGATGAACATGTGGCCCGGAGTGCGC; CTACNAGTGACTGAGGGCTGGGACGAGGACGGCCACACGCTCAGGATCACTCACTACGAA; GCCGTGCCTTGGANTCACTACTTCTGACCGCGACGCAACAGTATGGGTTGCTGGCGCGCC; TCCAATGAAAGCCGCTTCCACTGGGTTACTACAAGTCCGAACCACGTCCAGTGTCGGTCA; AGCTGAAACTCACTGA;
• Restriction Sites: Please inquire
• ACCN: NM_021044
• Insert Size: 1971 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021044.2, NP_066382.1
• RefSeq Size: 1971 bp
• RefSeq ORF: 1191 bp
• Locus ID: 50846
• UniProt ID: O43323
• Cytogenetics: 12q13.12
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Hedgehog signaling pathway
• Summary: This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010]