IKZF3 (NM_012481) Human Untagged Clone

SKU SC122788

IKZF3 (untagged)-Human IKAROS family zinc finger 3 (Aiolos) (IKZF3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: IKZF3
• Synonyms: AIO; AIOLOS; ZNFN1A3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012481 edited; CCACGCGTCCGGGTAGCCACAGCGGCAGCTCCAGCCCGGCCCGGCAGCGACATGGAAGAT; ATACAAACAAATGCGGAACTGAAAAGCACTCAGGAGCAGTCTGTGCCCGCAGAAAGTGCA; GCGGTTTTGAATGACTACAGTTTAACCAAATCTCATGAAATGGAAAATGTGGACAGTGGA; GAAGGCCCAGCCAATGAAGATGAAGACATAGGAGATGATTCAATGAAAGTGAAAGATGAA; TACAGTGAAAGAGATGAGAATGTTTTAAAGTCAGAACCCATGGGAAATGCAGAAGAGCCT; GAAATCCCTTACAGCTATTCAAGAGAATATAATGAATATGAAAACATTAAGTTGGAGAGA; CATGTTGTCTCATTCGATAGTAGCAGGCCAACCAGTGGAAAGATGAACTGCGATGTGTGT; GGATTATCCTGCATCAGCTTCAATGTCTTAATGGTTCATAAGCGAAGCCATACTGGTGAA; CGCCCATTCCAGTGTAATCAGTGTGGGGCATCTTTTACTCAGAAAGGTAACCTCCTCCGC; CACATTAAACTGCACACAGGGGAAAAACCTTTTAAGTGTCACCTCTGCAACTATGCATGC; CAAAGAAGAGATGCGCTCACGGGGCATCTTAGGACACATTCTGTGGAGAAACCCTACAAA; TGTGAGTTTTGTGGAAGGAGTTACAAGCAGAGAAGTTCCCTTGAGGAGCACAAGGAGCGC; TGCCGTACATTTCTTCAGAGCACTGACCCAGGGGACACTGCAAGTGCGGAGGCAAGACAC; ATCAAAGCAGAGATGGGAAGTGAAAGAGCTCTCGTACTGGACAGATTAGCAAGCAATGTG; GCAAAACGAAAAAGCTCAATGCCTCAGAAATTCATTGGTGAGAAGCGCCACTGCTTTGAT; GTCAACTATAATTCAAGTTACATGTATGAGAAAGAGAGTGAGCTCATACAGACCCGCATG; ATGGACCAAGCCATCAATAACGCCATCAGCTATCTTGGCGCCGAAGCCCTGCGCCCCTTG; GTCCAGACACCGCCTGCTCCCACCTCGGAGATGGTTCCAGTTATCAGCAGCATGTATCCC; ATAGCCCTCACCCGGGCTGAGATGTCAAACGGTGCCCCTCAAGAGCTGGAAAAGAAAAGC; ATCCACCTTCCAGAGAAGAGCGTGCCTTCTGAGAGAGGCCTCTCTCCCAACAATAGTGGC; CACGACTCCACGGACACTGACAGCAACCATGAAGAACGCCAGAATCACATCTATCAGCAA; AATCACATGGTCCTGTCTCGGGCCCGCAATGGGATGCCACTTCTGAAGGAGGTTCCCCGC; TCTTACGAACTCCTCAAGCCCCCGCCCATCTGCCCAAGAGACTCCGTCAAAGTGATCAAC; AAGGAAGGGGAGGTGATGGATGTGTATCGGTGTGACCACTGCCGCGTCCTCTTCCTGGAC; TATGTGATGTTCACGATTCACATGGGCTGCCACGGCTTCCGTGACCCTTTCGAGTGTAAC; ATGTGTGGATATCGAAGCCATGATCGGTATGAGTTCTCGTCTCACATAGCCAGAGGAGAA; CACAGAGCCCTGCTGAAGTGAATATCTGGTCTCAGGGATTGCTCCTATGTATTCAGCATC; GTTTCTAAAAACCAATGACCTCGCCTAACAGATTGCTCTCAAAACATACTCAGTTCCAAA; CTTCTTTTCATACCATTTTTAGCTGTGTTCACAGGGGTAGCCAGAGAAACACTGTCTTCC; TTCAGAAATTATTCGCAGGTCTAGCATATTATTACTTTTGTGAAACCTTTGTTTTCCCAT; CAGGGACTTGAATTTTATGGAATTTAAAAGCCAAAAAGGTATTTGGTCATTATCTTCTAC; AGCAGTGGAATGAGTGGTCCCGGAGATGTGCTATATGAAACATTCTTTCTGAGATATATC; AACCACACGTGGAAAAGCCTTTCAGTCATACATGCAAATCCACAAAGAGGAAGAGCTGAC; CAGCTGACCTTGCTGGGAAGCCTCACCCTTCTGCCCTTCACAGGCTGAAGGGTTAAGATC; TAATCTCCCTAATCTAAATGACAGTCTAAGAGTAAGTAAAAGAACAGCCATAAAATAAGT; ATCTGTTACGAGTAACTGAAGACCCCATTCTCCAAGCATCAGATCCATTTCCTATCACAA; CATTTTTAAAAAATGTCATCTGATGGCACTTCTGCTTCTGTCCTTTACCTTCCCATCTCC; AGTGAAAAGCTGAGCTGCTTTGGGCTAAACCAGTTGTCTATAGAAGAAAATCTATGCCAG; AAGAACTCATGGTTTTAAATATAGACCATCATCGAAACTCCAGAAATTTATCCACTGTGG; ATGATGACATCGCTTTCCTTTGGTCAAGGTTGGCAGAGCAAGGGTATAAAGGGGGAAATT; GTTTGGCAGCACCAACAGAAAACAAAACAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012481 unedited; CTTAGCATTAGTAATACGACTTTATATNAGGGCGGCACGCGGAATTCCCGGGGAATCGTC; GACCCACGCGTCCGGGTAGCCACAGCGGCAGCTCCAGCCCGGCCCGGCAGCGACATGGGA; AGATATACAAACAAATGCGGAACTGAAAAGCACTCAGGAGCAGTCTGTGCCCGCAGAAAG; TGCAGCGGTTTTGAATGACTACAGTTTAACCAAATCTCATGAAATGGAAAATGTGGACAG; TGGAGAAGGCCCAGCCAATGAAGATGAAGACATAGGAGATGATTCAATGAAAGTGAAAGA; TGAATACAGTGAAAGAGATGAGAATGTTTTAAAGTCAGAACCCATGGGAAATGCAGAAGA; GCCTGAAATCCCTTACAGCTATTCAAGAGAATATAATGAATATGAAAACATTAAGTTGGA; GAGACATGTTGTCTCATTCGATAGTAGCAGGCCAACCAGTGGAAAGATGAACTGCGATGT; GTGTGGATTATCCTGCATCAGCTTCAATGTCTTAATGGTTCATAAGCGAAGCCATACTGG; TGAACGCCCATTCCAGTGTAATCAGTGTGGAGCATCTTTTACTCAGAAAGGTAACCTCCT; CCGCCACATTAAACTGCACACAGGGGGAAAAACCTTTTAAGTGTCACCCTCTGCACTATG; CATGCCANAGAAGAGATGCCCTTACGGGGCATCTTTAGGACACATTCTGTGGAAAAAACC; CTACAAATGTGAAGTTTTTGGGGAAGGAGTTTACAAGCCAAAAAATTTCCCTTGAAGAAC; ACAAGGGACGCCTGCCGTACATTTCTTTCGGAACACTGACCCAAGGGACACCTGCAGTGC; GGGAGGCAAACAACCTCCAATCCAAATTGGGAAGGGAAAAAGCTCTCGTATCGGACAAAT; TAACAGCCATGGGGCAAAACAAAAAATCCATGGCTGCGAAATTCTT;
• Restriction Sites: Please inquire
• ACCN: NM_012481
• Insert Size: 2443 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012481.3, NP_036613.2
• RefSeq Size: 2437 bp
• RefSeq ORF: 1530 bp
• Locus ID: 22806
• UniProt ID: Q9UKT9
• Cytogenetics: 17q12-q21.1
• Summary: This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012]; Transcript Variant: This variant (1) encodes the longest isoform (1, also known as Aio-1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.