Nuclear Factor Erythroid Derived 2 (NFE2) (NM_006163) Human Untagged Clone

SKU SC122739

NFE2 (untagged)-Human nuclear factor (erythroid-derived 2), 45kDa (NFE2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Nuclear Factor Erythroid Derived 2
• Synonyms: NF-E2; p45
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006163 edited; GTGCGCCTGCTTGGGGCTCCTGTGCTCAGCTCAGCCTGAGCTTCCACACTCAGCGCTCAG; CAATGGCCCGGGGGCGGGGCGCGGTCCTCGCAGATTCTCAAAGGTAGCCGGGATCCTCGT; CCAGCAGTGTCAGCTCAGGCTCAGCCTCCCCAGAGACAACACCGGGAGCCTCATCTCTCT; CCTCACCCTGCTGTGACTCCACCACAGGTTTCTAGAGCCATCTGGGCTTTCCGGGAACCT; GGACCAGACTCTGGCCCAGTAGGATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGG; TGATACAGCTGTCCACTTCAGAGCTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGT; CCATCACCGAGCTGCAGGGTCTGAATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCC; CAGCTCCATACCTTGGACCTCCACCACCCACAACTTACTGCCCCTGCTCAATCCACCCAG; ATTCTGGCTTCCCACTTCCTCCACCACCTTATGAGCTCCCAGCATCCACATCCCATGTCC; CAGATCCCCCATACTCCTATGGCAACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCT; CAGGCCTGCTCAGTGAGCCGCTCCAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAG; CAGGGCCACCTAAGCCCCAAGAAGACCCAGAATCCGACTCAGGATTATCCCTCAACTATA; GCGATGCTGAATCTCTTGAGCTGGAGGGGACAGAGGCTGGTCGGCGGCGCAGCGAATATG; TAGAGATGTACCCAGTGGAGTACCCCTACTCACTCATGCCCAACTCCTTGGCCCACTCCA; ACTATACCTTGCCAGCTGCTGAGACCCCCTTGGCCTTAGAGCCCTCCTCAGGCCCTGTGC; GGGCTAAGCCCACTGCACGGGGGGAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCA; TGAAGATTCCTTTTCCTACGGACAAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGC; TATTGGCAAGGTACCCGCTGACAGAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGAC; GGGGCAAAAACAAGGTGGCAGCCCAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGC; AGCTGGAGCGGGAGCTGGAGCGGCTGACCAATGAACGGGAGCGGCTTCTCAGGGCCCGCG; GGGAGGCAGACCGGACCCTGGAGGTCATGCGCCAACAGCTGACAGAGCTGTACCGTGACA; TTTTCCAGCACCTTCGGGATGAATCAGGCAACAGCTACTCTCCTGAAGAGTACGCGCTGC; AACAGGCTGCCGATGGGACCATCTTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAG; ACTGAGCTGGCCCAGAGGGGTGGAACTGCTGATGGGATTTCCTTCATTCCCTTCTGATAA; AGGTACTCCCCAACCCTGAGTCCCAGAAGGAGCTGAGTTCTCTAGACCAGAAGAGGATGA; CAATGGCAACAAGTGTTTGGAAGTTCCAAGGTGTGTTCAAAGAGGCTTGCCTTGAGGGAG; GGCTGGAATCTGTCTTCCCTGACTCGGCTCCTCAGGTCTTTAGCCTCCACCTTGTCTAAG; CTTTGGTCTATAAAGTGCGCTACAGAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006163 unedited; NGAGACTTCATTTGTATACGACTTATATAGGGCGGCCGCGATTCGGCACGAGGGTGCGCC; TGTTTGGGGCTCCTGTGCTCAGCTCAGCCTGAGCTTCCACACTCAGCGCTCAGCAATGGC; CCGGGGGCGGGGCGCGGTCCTCGCAGATTCTCAAAGGTAGCCGGGATCCTCGTCCAGCAG; TGTCAGCTCAGGCTCAGCCTCCCCAGAGACAACACCGGGAGCCTCATCTCTCTCCTCACC; CTGCTGTGACTCCACCACATGTTTCTAGAGCCATCTGGGCTTTCCGGGAACCTGGACCAG; ACTCTGGCCCAGTAGGATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACA; GCTGTCCACTTCAGAGCTAGGAGAGATGGAACTGACTTGGCAGGAGATCATGTCCATCAC; CGAGCTGCAGGGTCTGAATGCTCCAAGTGAGCCATCATTTGAGCCCCAAGCCCCAGCTCC; ATACCTTGGACCTCCACCACCCACAACTTACTGCCCCTGCTCAATCCACCCAGATTCTGG; CTTCCCACTTCCTCCACCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAGATCC; CCCATACTCCTATGGCAACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCT; GCTCAGTGAGCCGCTCCAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCC; ACCTAAGCCCCAAGAAGACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGC; TGAATCTCTTGAGCTGGAGGGGACAGAGGCTGGTCGGCGGCGCANCGAATATGTAGAGAT; GTACCCAGTGGAGTACCCCTACTCACTCATGCCCAACTNCTTGA;
• Restriction Sites: Please inquire
• ACCN: NM_006163
• Insert Size: 1663 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006163.1, NP_006154.1
• RefSeq Size: 1678 bp
• RefSeq ORF: 1122 bp
• Locus ID: 4778
• UniProt ID: Q16621
• Cytogenetics: 12q13.13
• Protein Families: Transcription Factors
• Summary: Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 2 and 3 encode the same protein.