Metallothionein (MT1A) (NM_005946) Human Untagged Clone

SKU SC122731

MT1A (untagged)-Human metallothionein 1A (MT1A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Metallothionein
• Synonyms: MT-1A; MT-IA; MT1; MT1S; MTC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005946 edited; AAGCCTTCCACGTGCGCCTTATAGCCTCTCAACTTCTTGCTTGGGATCTCCAACCTCACC; GCGGCTCGAAATGGACCCCAACTGCTCCTGCGCCACTGGTGGCTCCTGCACCTGCACTGG; CTCCTGCAAATGCAAAGAGTGCAAATGCAACTCCTGCAAGAAGAGCTGCTGCTCCTGCTG; CCCCATGAGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCATCAGAGAAGTG; CAGCTGCTGTGCCTGATGTCCGGACAGCCCTGCTCGAAGATATAGAAAGAGTGACCTGCA; CAAACTTGGAATTTTTTTTCCATACAACCCTGACCCATTTACTGTATTTTTTTTAATGAA; ATATGTGAATGATAATAAAAGTTGCTGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005946 unedited; AGGTCGAATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCCATTACGGCCGGGGA; AGCCTTCCACGTGCGCCTTATAGCCTCTCAACTTCTTGCTTGGGATCTCCAACCTCACCG; CGGCTCGAAATGGACCCCAACTGCTCCTGCGCCACTGGTGGCTCCTGCACCTGCACTGGC; TCCTGCAAATGCAAAGAGTGCAAATGCAACTCCTGCAAGAAGAGCTGCTGCTCCTGCTGC; CCCATGAGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCATCAGAGAAGTGC; AGCTGCTGTGCCTGATGTCCGGACAGCCCTGCTCGAAGATATAGAAAGAGTGACCTGCAC; AAACTTGGAATTTTTTTTCCATACAACCCTGACCCATTTACTGTATTTTTTTTAATGAAA; TATGTGAATGATAATAAAAGTTGCTGACTTANNNNAAAAANNNNAAAAAAAAAAAAAAAA; AAAAAAAAAAACCCCCNNAAAAAAAAAAAAAAAAAAAAAAAAACATGTCGGCCGCCTCGG; CCCTCAACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCA; TCCCTGTGACCCCTCCCAAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCAAC; CAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGCCTGACTAGGTGTCCTTCTATAT; ATTATGGGTGGAGGGGGGTGGGTTATTTGAACAAAGGGCCAAATTTGGAAAAAAACCCTG; TGGGGCCCGCGGGTCCATTGGGAACACAACCTGGAATGGAGTGGCAAAACTTTGGCCTCC; GGGAATCCCCCCCTCCCTGGTTTAAGCCAATTTCCCTGCCTCAACCTCCCCAATTGTTGG; GTTTCCCGGCTGCTTGAACAGCCTAT;
• Restriction Sites: Please inquire
• ACCN: NM_005946
• Insert Size: 465 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005946.2, NP_005937.2
• RefSeq Size: 468 bp
• RefSeq ORF: 186 bp
• Locus ID: 4489
• UniProt ID: P04731
• Cytogenetics: 16q13
• Summary: This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. The conserved cysteine residues co-ordinate metal ions using mercaptide linkages. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. Disruption of two metallothionein genes in mouse resulted in defects in protection against heavy metals, oxidative stress, immune reactions, carcinogens, and displayed obesity. [provided by RefSeq, Sep 2017]