Metallothionein (MT1A) (NM_005946) Human Untagged Clone

CAT#: SC122731

MT1A (untagged)-Human metallothionein 1A (MT1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Metallothionein
• Synonyms: MT-1A; MT-IA; MT1; MT1S; MTC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005946 edited
  AAGCCTTCCACGTGCGCCTTATAGCCTCTCAACTTCTTGCTTGGGATCTCCAACCTCACC
  GCGGCTCGAAATGGACCCCAACTGCTCCTGCGCCACTGGTGGCTCCTGCACCTGCACTGG
  CTCCTGCAAATGCAAAGAGTGCAAATGCAACTCCTGCAAGAAGAGCTGCTGCTCCTGCTG
  CCCCATGAGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCATCAGAGAAGTG
  CAGCTGCTGTGCCTGATGTCCGGACAGCCCTGCTCGAAGATATAGAAAGAGTGACCTGCA
  CAAACTTGGAATTTTTTTTCCATACAACCCTGACCCATTTACTGTATTTTTTTTAATGAA
  ATATGTGAATGATAATAAAAGTTGCTGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005946 unedited
  AGGTCGAATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCCATTACGGCCGGGGA
  AGCCTTCCACGTGCGCCTTATAGCCTCTCAACTTCTTGCTTGGGATCTCCAACCTCACCG
  CGGCTCGAAATGGACCCCAACTGCTCCTGCGCCACTGGTGGCTCCTGCACCTGCACTGGC
  TCCTGCAAATGCAAAGAGTGCAAATGCAACTCCTGCAAGAAGAGCTGCTGCTCCTGCTGC
  CCCATGAGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCATCAGAGAAGTGC
  AGCTGCTGTGCCTGATGTCCGGACAGCCCTGCTCGAAGATATAGAAAGAGTGACCTGCAC
  AAACTTGGAATTTTTTTTCCATACAACCCTGACCCATTTACTGTATTTTTTTTAATGAAA
  TATGTGAATGATAATAAAAGTTGCTGACTTANNNNAAAAANNNNAAAAAAAAAAAAAAAA
  AAAAAAAAAAACCCCCNNAAAAAAAAAAAAAAAAAAAAAAAAACATGTCGGCCGCCTCGG
  CCCTCAACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCA
  TCCCTGTGACCCCTCCCAAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCAAC
  CAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGCCTGACTAGGTGTCCTTCTATAT
  ATTATGGGTGGAGGGGGGTGGGTTATTTGAACAAAGGGCCAAATTTGGAAAAAAACCCTG
  TGGGGCCCGCGGGTCCATTGGGAACACAACCTGGAATGGAGTGGCAAAACTTTGGCCTCC
  GGGAATCCCCCCCTCCCTGGTTTAAGCCAATTTCCCTGCCTCAACCTCCCCAATTGTTGG
  GTTTCCCGGCTGCTTGAACAGCCTAT
  
• Restriction Sites: Please inquire
• ACCN: NM_005946
• Insert Size: 465 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005946.2, NP_005937.2
• RefSeq Size: 468 bp
• RefSeq ORF: 186 bp
• Locus ID: 4489
• UniProt ID: P04731
• Cytogenetics: 16q13
• Gene Summary: This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. The conserved cysteine residues co-ordinate metal ions using mercaptide linkages. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. Disruption of two metallothionein genes in mouse resulted in defects in protection against heavy metals, oxidative stress, immune reactions, carcinogens, and displayed obesity. [provided by RefSeq, Sep 2017]