ALS (IGFALS) (NM_004970) Human Untagged Clone

SKU SC122694

IGFALS (untagged)-Human insulin-like growth factor binding protein, acid labile subunit (IGFALS), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ALS
• Synonyms: ACLSD; ALS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004970 edited; GGAAATCCAGAGGGCAGGGGTGGCCGGCACAGCAGACGTACCCTCCCTCGCTGCCTGCCT; GCAGCCTGCCCTGCATGCAGGATGGCCCTGAGGAAAGGAGGCCTGGCCCTGGCGCTGCTG; CTGCTGTCCTGGGTGGCACTGGGCCCCCGCAGCCTGGAGGGAGCAGACCCCGGAACGCCG; GGGGAAGCCGAGGGCCCAGCGTGCCCGGCCGCCTGTGTCTGCAGCTACGATGACGACGCG; GATGAGCTCAGCGTCTTCTGCAGCTCCAGGAACCTCACGCGCCTGCCTGATGGAGTCCCG; GGCGGCACCCAAGCCCTGTGGCTGGACGGCAACAACCTCTCGTCCGTCCCCCCGGCAGCC; TTCCAGAACCTCTCCAGCCTGGGCTTCCTCAACCTGCAGGGCGGCCAGCTGGGCAGCCTG; GAGCCACAGGCGCTGCTGGGCCTAGAGAACCTGTGCCACCTGCACCTGGAGCGGAACCAG; CTGCGCAGCCTGGCACTCGGCACGTTTGCACACACGCCCGCGCTGGCCTCGCTCGGCCTC; AGCAACAACCGTCTGAGCAGGCTGGAGGACGGGCTCTTCGAGGGCCTCGGCAGCCTCTGG; GACCTCAACCTCGGCTGGAATAGCCTGGCGGTGCTCCCCGATGCGGCGTTCCGCGGCCTG; GGCAGCCTGCGCGAGCTGGTGCTGGCGGGCAACAGGCTGGCCTACCTGCAGCCCGCGCTC; TTCAGCGGCCTGGCCGAGCTCCGGGAGCTGGACCTGAGCAGGAACGCGCTGCGGGCCATC; AAGGCAAACGTGTTCGTGCAGCTGCCCCGGCTCCAGAAACTCTACCTGGACCGCAACCTC; ATCGCTGCCGTGGCCCCGGGCGCCTTCCTGGGCCTGAAGGCGCTGCGATGGCTGGACCTG; TCCCACAACCGCGTGGCTGGCCTCCTGGAGGACACGTTCCCCGGTCTGCTGGGCCTGCGT; GTGCTGCGGCTGTCCCACAACGCCATCGCCAGCCTGCGGCCCCGCACCTTCAAGGACCTG; CACTTCCTGGAGGAGCTGCAGCTGGGCCACAACCGCATCCGGCAGCTGGCTGAGCGCAGC; TTTGAGGGCCTGGGGCAGCTTGAGGTGCTCACGCTAGACCACAACCAGCTCCAGGAGGTC; AAGGCGGGCGCTTTCCTCGGCCTCACCAACATGGCGGTCATGAACCTCTCTGGGAACTGT; CTCCGGAACCTTCCGGAGCAGGTGTTCCGGGGCCTGGGCAAGCTGCACAGCCTGCACCTG; GAGGGCAGCTGCCTGGGACGCATCCGCCCGCACACCTTCACCGGCCTCTCGGGGCTCCGC; CGACTCTTCCTCAAGGACAACGGCCTCGTGGGCATTGAGGAGCAGAGCCTGTGGGGGCTG; GCGGAGCTGCTGGAGCTCGACCTGACCTCCAACCAGCTCACGCACCTGCCCCACCGCCTC; TTCCAGGGCCTGGGCAAGCTGGAGTACCTGCTGCTCTCCCGCAACCGCCTGGCAGAGCTG; CCGGCGGACGCCCTGGGCCCCCTGCAGCGGGCCTTCTGGCTGGACGTCTCGCACAACCGC; CTGGAGGCATTGCCCAACAGCCTCTTGGCACCACTGGGGCGGCTGCGCTACCTCAGCCTC; AGGAACAACTCACTGCGGACCTTCACGCCGCAGCCCCCGGGCCTGGAGCGCCTGTGGCTG; GAGGGTAACCCCTGGGACTGTGGCTGCCCTCTCAAGGCGCTGCGGGACTTCGCCCTGCAG; AACCCCAGTGCTGTGCCCCGCTTCGTCCAGGCCATCTGTGAGGGGGACGATTGCCAGCCG; CCCGCGTACACCTACAACAACATCACCTGTGCCAGCCCGCCCGAGGTCGTGGGGCTCGAC; CTGCGGGACCTCAGCGAGGCCCACTTTGCTCCCTGCTGACCAGGTCCCCGGACTCAAGCC; CCGGACTCAGGCCCCCACCTGGCTCACCTTGTGCTGGGGACAGGTCCTCAGTGTCCTCAG; GGGCCTGCCCAGTGCACTTGCTGGAAGACGCAAGGGCCTGATGGGGTGGAAGGCATGGCG; GCCCCCCCAGCTGTCATCAATTAAAGGCAAAGGCAATCGAATCTAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004970 unedited; GCGNAATTCCCGGGTATGGAAATCCAGAGTGCAGGGGTGGCCGGCACAGCAGACGTACCC; TCCCTCGCTGCCTGCCTGCAGCCTGCCCTGCATGCAGGATGGCCCTGAGGAAAGGAGGCC; TGGCCCTGGCGCTGCTGCTGCTGTCCTGGGTGGCACTGGGCCCCCGCAGCCTGGAGGGAG; CAGACCCCGGAACGCCGGGGGAAGCCGAGGGCCCAGCGTGCCCGGCCTCCTGTGTCTGCA; GCTACGATGACGACGCGGATGAGCTCAGCGTCTTCTGCAGCTCCAGGAACCTCACGCGCC; TGCCTGATGGAGTCCCGGGCGGCACCCAAGCCCTGTGGCTGGACGGCAACAACCTCTCGT; CCGTCCCCCCGGCAGCCTTCCAGAACCTCTCCAGCCTGGGCTTCCTCAACCTGCAGGGCG; GCCAGCTGGGCAGCCTGGAGCCACAGGCGCTGCTGGGCCTAGAGAACCTGTGCCACCTGC; ACCTGGAGCGGAACCAGCTGCGCAGCCTGGCACTCGGCACGTTTGCACACACGCCCGCGC; TGGCCTCGCTCGGCCTCAGCAACAACCGTCTGAGCAGGCTGGAGGACGGGCTCTTCGAGG; GCCTCGGCAGCCTCTGGGACCTCAACCTCGGCTGGAATAGCCTGGCGGTGCTCCCCGATG; CGGCGTTCCGCGGCCTGGGCAGCCTGCGCGAGCTGGTGCTGGCGGGCAACAGGCTGGCCT; ACCTGCAGCCCGCGCTCTTCAGCGGCCTGGCCGAGCTCCGGGAGCTGGACCTGAGCCAGA; ACGCGCTGCGGGCCATCAAGGCAAACGTGTTCGTGCAGCTGCCCCGGCTCCAGAAACTCT; ACCTGGACCGCAACCTCATCGCTGCCGTGGGCCCCGG;
• Restriction Sites: Please inquire
• ACCN: NM_004970
• Insert Size: 2168 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004970.1, NP_004961.1
• RefSeq Size: 2125 bp
• RefSeq ORF: 1818 bp
• Locus ID: 3483
• UniProt ID: P35858
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Secreted Protein
• Summary: The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]; Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.