NAPSIN A (NAPSA) (NM_004851) Human Untagged Clone

SKU SC122691

NAPSA (untagged)-Human napsin A aspartic peptidase (NAPSA)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NAPSIN A
• Synonyms: KAP; Kdap; NAP1; NAPA; SNAPA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004851 edited; GACACCCAGGTCCCCAGCGATGTCTCCACCACCGCTGCTGCAACCCCTGCTGCTGCTGCT; GCCTCTGCTGAATGTGGAGCCTTCCGGGGCCACACTGATCCGCATCCCTCTTCATCGAGT; CCAACCTGGACGCAGGACCCTGAACCTACTGAGGGGATGGAGAGAACCAGCAGAGCTCCC; CAAGTTGGGGGCCCCATCCCCTGGGGACAAGCCCATCTTCGTACCTCTCTCGAACTACAG; GGATGTGCAGTATTTTGGGGAAATTGGGCTGGGAACGCCTCCACAAAACTTCACTGTTGC; CTTTGACACTGGCTCCTCCAATCTCTGGGTCCCGTCCAGGAGATGCCACTTCTTCAGTGT; GCCCTGCTGGTTACACCACCGATTTGATCCCAAAGCCTCTAGCTCCTTCCAGGCCAATGG; GACCAAGTTTGCCATTCAATATGGAACTGGGCGGGTAGATGGAATCCTGAGCGAGGACAA; GCTGACTATTGGTGGAATCAAGGGTGCATCAGTGATTTTCGGGGAGGCTCTCTGGGAGCC; CAGCCTGGTCTTCGCTTTTGCCCATTTTGATGGGATATTGGGCCTCGGTTTTCCCATTCT; GTCTGTGGAAGGAGTTCGGCCCCCGATGGATGTACTGGTGGAGCAGGGGCTATTGGATAA; GCCTGTCTTCTCCTTTTACCTCAACAGGGACCCTGAAGAGCCTGATGGAGGAGAGCTGGT; CCTGGGGGGCTCGGACCCGGCACACTACATCCCACCCCTCACCTTCGTGCCAGTCACGGT; CCCCGCCTACTGGCAGATCCACATGGAGCGTGTGAAGGTGGGCCCAGGGCTGACTCTCTG; TGCCAAGGGCTGTGCTGCCATCCTGGATACGGGCACGTCCCTCATCACAGGACCCACTGA; GGAGATCCGGGCCCTGCATGCAGCCATTGGGGGAATCCCCTTGCTGGCTGGGGAGTACAT; CATCCTGTGCTCGGAAATCCCAAAGCTCCCCGCAGTCTCCTTCCTTCTTGGGGGGGTCTG; GTTTAACCTCACGGCCCATGATTACGTCATCCAGACTACTCGAAATGGCGTCCGCCTCTG; CTTGTCCGGTTTCCAGGCCCTGGATGTCCCTCCGCCTGCAGGGCCCTTCTGGATCCTCGG; TGACGTCTTCTTGGGGACGTATGTGGCCGTCTTCGACCGCGGGGACATGAAGAGCAGCGC; CCGGGTGGGCCTGGCGCGCGCTCGCACTCGCGGAGCGGACCTCGGATGGGGAGAGACTGC; GCAGGCGCAGTTCCCCGGGTGACGCCCAAGTGAAGCGCATGCGCAGCGGGTGGTCGCGGA; GGTCCTGCTACCCAGTAAAAATCCACTATTTCCATTGGAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004851 unedited; GGAGTTGGGATTTGTAATACGACTTTATATAGGGCGGCGCGCGAATTCGCCATTACGGCC; GGGGGACACCCAGGTCCCCAGCGATGTCTCCACCACCGCTGCTGCAACCCCTGCTGCTGC; TGCTGCCTCTGCTGAATGTGGAGCCTTCCGGGGCCACACTGATCCGCATCCCTCTTCATC; GAGTCCAACCTGGACGCAGGACCCTGAACCTACTGAGGGGATGGAGAGAACCAGCAGAGC; TCCCCAAGGTGGGGGCCCCATCCCCTGGGGACAAGCCCATCTTCGTACCTCTCTCGAACT; ACAGGGATGTGCAGTATTTTGGGGAAATTGGGCTGGGAACGCCTCCACAAAACTTCACTG; TTGCCTTTGACACTGGCTCCTCCAATCTCTGGGTCCCGTCCAGGAGATGCCACTTCTTCA; GTGTGCCCTGCTGGTTACACCACCGATTTGATCCCAAAGCCTCTAGCTCCTTCCAGGCCA; ATGGGACCAAGTTTGCCATTCAATATGGAACTGGGCGGGTAGATGGAATCCTGAGCGAGG; ACAAGCTGACTATTGGTGGAATCAAGGGTGCATCAGTGATTTTCGGGGAGGCTCTCTGGG; AGCCCAGCCTGGTCTTCGCTTTTGCCCATTTTGATGGGATATTGGGCCTCGGTTTTCCCA; TTCTGTCTGTGGAAGGAGTTCGGCCCCCGATGGATGTACTGGTGGAGCAGGGGCTATTGG; ATAAGCCTGTCTTCTCCTTTTACCTCANCAGGGACCCTGAAAAGCCTGATGGAGGAGACT; GGGTCTGGGGGGCTCCGACCCGGCACACTACATCCCACCCCTCACCTTCGTGCCAGTCAC; GGTCCCCNGGCTACTGGCAGATCCACATGGAGCGTGTGAAAGGTGGGCCCCAGGGCTGAC; TCTCTGGTGGCAAGGGCTGTGCTGCA;
• Restriction Sites: Please inquire
• ACCN: NM_004851
• Insert Size: 1389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004851.1, NP_004842.1
• RefSeq Size: 1438 bp
• RefSeq ORF: 1263 bp
• Locus ID: 9476
• UniProt ID: O96009
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Summary: This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma. [provided by RefSeq, Feb 2016]