TIMM8A (NM_004085) Human Untagged Clone

SKU SC122667

TIMM8A (untagged)-Human translocase of inner mitochondrial membrane 8 homolog A (yeast) (TIMM8A), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TIMM8A
• Synonyms: DDP; DDP1; DFN1; MTS; TIM8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004085 edited; GGGGGAGACACGTGAAGGTCGGTGCGGAGTTCGTCTCTGCAAGCTTGGTCGCCCTGGGAT; GGATTCCTCCTCCTCTTCCTCCGCGGCGGGTTTGGGTGCAGTGGACCCGCAGTTGCAGCA; TTTCATCGAGGTAGAGACTCAAAAGCAGCGCTTCCAGCAGCTGGTGCACCAGATGACTGA; ACTTTGTTGGGAGAAGTGCATGGACAAGCCTGGGCCAAAGTTGGACAGTCGGGCTGAGGC; CTGTTTTGTGAACTGCGTTGAGCGCTTCATTGATACAAGCCAGTTCATCTTGAATCGACT; GGAACAGACCCAGAAATCCAAGCCAGTTTTCTCAGAAAGCCTTTCTGACTGATCTCAGCA; TTACCCCTTTGGAAAAGGAAGGTAGTTCAAGAAATGAAGAGCTGTTGATGGGATGATTGA; AGAAACAGCTATGAGAGGATTGGCTCCCATCTTTTGTTACTCTTGGGACATCCTGTCATC; TGAGAATGAACAAAGACCAATTTTTTGTGTGTGAAGCTTAAGGGTCATATGTTTGCTTGT; ATTTTTTAATGCTAATCTTGTGAAAATAATTGACAGGCGAAAGAAAACTCTATTTAGATG; CATATTACTGTACATGGGACTATGCTTTTCTCAAAGCCCCATTAACTGCTTCCTATAATT; TTGATAGTGGGACCACATACGTAAAAATCTCTCATTTGTGTGGAGTCATTTCTGATTTCA; GGGGAGATCCTTGTGTTTATCAGAAAGGGCAGAAGTAGGGGAAGAATAATTTGGTATCCT; TATCTAGTGTTTGATTGTCAATGCTGGAGAAAAATATCTGTAAGAGTGTTTATACAGTAC; ACTTCAGTTATCTTGATCTCCCTTTCCTATATGATGATTTGCTTAAATATCCATATTAAG; TAAGTCTCAAGGTAGGGTAGGCAGCCTGAGAGTCTAGAGGCCTTTAGTTATAAAGGAATC; TAGCCAGTGAACATAATTCTTATTACTAGACTGCCACAAGGAAGAAATTAACTTACCCTG; TATATCAGGGTACAAAAAATTCAGTGATGTGCCTAAATAAGTTATAAAGATTTAGGCCAA; TCAGAAGCTAACAGCAGTTTCAGGTAGAGGTGCATGCCTAATGTTAGTTAGTGTAGATTC; CATTTACTGCATTCTTCTGATCACTGAAATAAAAGCTATATAAGATTAAAAAAAAAAATC; AAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004085 unedited; GAGATCAGGATTTGTNATACGACTCTACTATAGGCGGCCGCGAAATTCGCCATTACGGCC; GGGGGAGACACGTGAAGGTCGGTGCGGAGTTCGTCTCTGCAAGCTTGGTCGCCCTGGGAT; GGATTCCTCCTCCTCTTCCTCCGCGGCGGGTTTGGGTGCAGTGGACCCGCAGTTGCAGCA; TTTCATCGAGGTAGAGACTCAAAAGCAGCGCTTCCAGCAGCTGGTGCACCAGATGACTGA; ACTTTGGTGGGAGAAGTGCATGGACAAGCCTGGGCCAAAGTTGGACAGTCGGGCTGAGGC; CTGTTTTGTGAACTGCGTTGAGCGCTTCATTGATACAAGCCAGTTCATCTTGAATCGACT; GGAACAGACCCAGAAATCCAAGCCAGTTTTCTCAGAAAGCCTTTCTGACTGATCTCAGCA; TTACCCCTTTGGAAAAGGAAGGTAGTTCAAGAAATGAAGAGCTGTTGATGGGATGATTGA; AGAAACAGCTATGAGAGGATTGGCTCCCATCTTTTGTTACTCTTGGGACATCCTGTCATC; TGAGAATGAACAAAGACCAATTTTTTGTGGGTGAAGCTTAAGGGCATATGTTTGCTTGAA; TTTTTAATGCTAATCCTTGGAAAATATTGACAGGCAAAAGAAAACTCCATTTAGAGGCTA; ATACTGTGCATGGGGCTATGCTTTTTCTAGAGGCGCCATTAACTGGTTCCCAAAATTTTG; AGAGGGGGACCACATACGTAAAAATCTCTCATTTGTGTGGGGGCCATTCCGGATTCCGGG; GGGAATGCCTGGGTTTAACAAAAAGGCCAGAAGATAGGGAAAGAAAATTTGGGGACCTGA; ACTAGTGTGTTGATGGTCAAGCCTGGAGAAAACAATCTGGTAGAAGTGGTTGATCCGGAA; CCCTCGAGTGTTCCTGGTGCCCCCTTN;
• Restriction Sites: Please inquire
• ACCN: NM_004085
• Insert Size: 1227 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004085.2, NP_004076.1
• RefSeq Size: 1168 bp
• RefSeq ORF: 294 bp
• Locus ID: 1678
• UniProt ID: O60220
• Cytogenetics: Xq22.1
• Protein Families: Druggable Genome
• Summary: This translocase is involved in the import and insertion of hydrophobic membrane proteins from the cytoplasm into the mitochondrial inner membrane. The gene is mutated in Mohr-Tranebjaerg syndrome/Deafness Dystonia Syndrome (MTS/DDS) and it is postulated that MTS/DDS is a mitochondrial disease caused by a defective mitochondrial protein import system. Defects in this gene also cause Jensen syndrome; an X-linked disease with opticoacoustic nerve atrophy and muscle weakness. This protein, along with TIMM13, forms a 70 kDa heterohexamer. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2009]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).