B3GALT4 (NM_003782) Human Untagged Clone

SKU SC122662

B3GALT4 (untagged)-Human UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4 (B3GALT4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: B3GALT4
• Synonyms: BETA3GALT4; GALT2; GALT4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003782 edited; GAGTGCCGCAGTCGGCCAGCCATGGAGCGGAGCTTGCTGGCGGCGAGGCCGCGGCGACAA; GGTAGCCACCCCCGCAGCATGCCTCGACCGCGGTCCGCAGCTGCACCGCCTCTCCCCGCC; CCCCAGGGTGCGCTGGTCCCGGTCGCGCGCTCAGACCTCCGCATCCCGGGCGTGGTCGGT; TAAGTCCCCGGCCGTGACCCAGGCCCGGGGAGCTAGTCTCCGCCCTTCGCTCTTACGGAT; CCCCTCGGAGTACGCCGCACCATGCAGCTCAGGCTCTTCCGGCGCCTCCTTCTCGCCGCT; TTGCTGCTGGTGATCGTCTGGACCCTCTTCGGGCCTTCGGGGTTGGGGGAGGAGCTGCTG; AGCCTCTCACTAGCCTCCCTGCTCCCAGCCCCCGCCTCACCGGGGCCGCCCCTGGCCCTG; CCCCGCCTCTTGATCCCCAACCAGGAAGCTTGCAGTGGTCCCGGGGCCCCTCCCTTCCTG; CTCATCCTGGTGTGCACGGCTCCGGAGAACCTGAACCAGAGAAACGCCATTCGGGCTTCG; TGGGGCGGGCTGCGCGAGGCCCGGGGGCTCAGGGTACAGACGCTATTCTTGCTGGGAGAG; CCGAACGCACAGCACCCCGTGTGGGGTTCCCAGGGGAGTGACCTGGCCTCGGAGTCAGCA; GCCCAGGGGGATATCTTGCAGGCCGCCTTCCAGGACTCCTACCGCAACCTCACCCTAAAG; ACCCTCAGCGGGCTGAACTGGGCTGAGAAACACTGCCCCATGGCCCGATACGTCCTCAAG; ACGGACGATGATGTGTATGTCAACGTCCCTGAACTGGTATCAGAGCTGGTCTTGCGAGGG; GGCCGTTGGGGGCAATGGGAGAGAAGCACGGAACCCCAGAGAGAGGCTGAGCAGGAAGGA; GGCCAGGTTTTGCACAGCGAGGAAGTGCCTCTTCTGTACTTGGGCCGGGTGCACTGGCGC; GTGAACCCCTCTCGGACACCGGGGGGCAGGCACCGCGTATCAGAGGAGCAGTGGCCTCAC; ACCTGGGGCCCCTTTCCACCCTATGCCTCAGGCACGGGGTATGTGCTGTCAGCGTCTGCT; GTGCAGCTCATTCTCAAGGTGGCCAGCCGGGCACCCCTTCTCCCATTAGAGGATGTCTTT; GTGGGGGTAAGTGCCCGACGAGGAGGCCTCGCCCCAACACAGTGTGTCAAGCTGGCTGGT; GCCACCCACTACCCGCTAGACCGGTGCTGCTATGGGAAATTCCTGCTGACGTCCCACAGG; CTGGACCCCTGGAAGATGCAGGAAGCCTGGAAGCTGGTGGGTGGCTCTGACGGGGAAAGG; ACTGCGCCCTTTTGCTCCTGGTTCCAGGGAGTCCTGGGCATCCTGCGGTGTCGAGCAATA; GCCTGGCTTCAGAGCTGAGAGTGCCTGGGGCCACAGGAAAGGCAGGAACAGGACCTTCTC; TCTCCCAGGCCCAACGCAGGGGCCCTCACTGGCTGCAGCTGATCTGTTTCCTTATACCAG; ATCCTCAGTCTCACTAAAGACAGCGATATGGGAGACACCCAGGGGCCTGGCCCGCCAGCC; CAAAAGATGGTCATCGGGAAGAAAAAAAGAAAAAAATGCTGCAGTTGTTCTCTCAAGCTA; GGGCAGAAGAGGGGTGTCAAGCTCCTCAATAAACTTGTCTCCACTTCAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003782 unedited; GTACGGATTTGTAAACCATTATATAGGCGGCCGCGAATTCCCGGGGTGAGTGCCGCAGTC; GGCCAGCCATGGAGCGGAGCTTGCTGGCGGCGAGGCCGCGGCGACAAGGTAGCCACCCCC; GCAGCATGCCTCGACCGCGGTCCGCAGCTGCACCGCCTCTCCCCGCCCCCCATGGTGCGC; TGGTCCCGGTCGCGCGCTCAGACCTCCGCATCCCGGGCGTGGTCGGTTAAGTCCCCGGCC; GTGACCCAGGCCCGGGGAGCTAGTCTCCGCCCTTCGCTCTTACGGATCCCCTCGGAGTAC; GCCGCACCATGCAGCTCATGCTCTTCCGGCGCCTCCTTCTCGCCGCTTTGCTGCTGGTGA; TCGTCTGGACCCTCTTCGGGCCTTCGGGGTTGGGGGAGGAGCTGCTGAGCCTCTCACTAG; CCTCCCTGCTCCCAGCCCCCGCCTCACCGGGGCCGCCCCTGGCCCTGCCCCGCCTCTGGA; TCCCAACCAGGAAGCTTGGAGTGGTCCCGGGGCCCCTCCCTTCCTGCTAATCCTGGGGGG; CACGGCTCCGGAAACCTGAACCAGAGAAACGCCCTTCGGGCTTCTTGGGGGCGGCTGCGC; CAGGCCCGGGGGCCTCAGGTACAGACCCTATTCTTGTTGGGAGACCCGAACGCACAGCAC; CCCCGGGGGGGGTTCCCAAGGGGAGTGGACCTGGCCTCCGGAGTCAGCAGCCCCAGGGGG; ATATCCTTGCAGGCCGCCCTTCCATGACTCCTAACCGAACCTCCACCCTAAAAACCTTAA; GCGGGCTGGACCGGGGCTGGAAAACCCGTGCCCATGGCCCGATTCCTCCTCCAAACGGAC; AATTATGTTTTTGTAAACGTCCCCGAACTGGTGATAAAACTGGTCCTTCAAAGGGGCCCC; TTTGGGGCCCATGCGAGAAAATCACGGACCCCCGCGATGAGGG;
• Restriction Sites: Please inquire
• ACCN: NM_003782
• Insert Size: 1703 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003782.3, NP_003773.1
• RefSeq Size: 1704 bp
• RefSeq ORF: 1137 bp
• Locus ID: 8705
• UniProt ID: O96024
• Cytogenetics: 6p21.32
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq, Jul 2008]