Fc epsilon RI (FCER1A) (NM_002001) Human Untagged Clone

SKU SC122614

FCER1A (untagged)-Human Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide (FCER1A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Fc epsilon RI
• Synonyms: FCE1A; FcERI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002001 edited; ACAGTAAGCACCAGGAGTCCATGAAGAAGATGGCTCCTGCCATGGAATCCCCTACTCTAC; TGTGTGTAGCCTTACTGTTCTTCGCTCCAGATGGCGTGTTAGCAGTCCCTCAGAAACCTA; AGGTCTCCTTGAACCCTCCATGGAATAGAATATTTAAAGGAGAGAATGTGACTCTTACAT; GTAATGGGAACAATTTCTTTGAAGTCAGTTCCACCAAATGGTTCCACAATGGCAGCCTTT; CAGAAGAGACAAATTCAAGTTTGAATATTGTGAATGCCAAATTTGAAGACAGTGGAGAAT; ACAAATGTCAGCACCAACAAGTTAATGAGAGTGAACCTGTGTACCTGGAAGTCTTCAGTG; ACTGGCTGCTCCTTCAGGCCTCTGCTGAGGTGGTGATGGAGGGCCAGCCCCTCTTCCTCA; GGTGCCATGGTTGGAGGAACTGGGATGTGTACAAGGTGATCTATTATAAGGATGGTGAAG; CTCTCAAGTACTGGTATGAGAACCACAACATCTCCATTACAAATGCCACAGTTGAAGACA; GTGGAACCTACTACTGTACGGGCAAAGTGTGGCAGCTGGACTATGAGTCTGAGCCCCTCA; ACATTACTGTAATAAAAGCTCCGCGTGAGAAGTACTGGCTACAATTTTTTATCCCATTGT; TGGTGGTGATTCTGTTTGCTGTGGACACAGGATTATTTATCTCAACTCAGCAGCAGGTCA; CATTTCTCTTGAAGATTAAGAGAACCAGGAAAGGCTTCAGACTTCTGAACCCACATCCTA; AGCCAAACCCCAAAAACAACTGATATAATTACTCAAGAAATATTTGCAACATTAGTTTTT; TTCCAGCATCAGCAATTGCTACTCAATTGTCAAACACAGCTTGCAATATACATAGAAACG; TCTGTGCTCAAGGATTTATAGAAATGCTTCATTAAACTGAGTGAAACTGGTTAAGTGGCA; TGTAATAGTAAGTGCTCAATTAACATTGGTTGAATAAATGAGAGAATGAATAGATTCATT; TATTAGCATTTGTAAAAGAGATGTTCAATTTCAATAAAATAAATATAAAACCAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002001 unedited; NGACACGTTTACATTTGTAAACGACTCCTATAGGCGGCCGCGNAATTCGCCATTACGGCC; GGGGACAGTAAGCACCAGGAGTCCATGAAGAAGAGGCTCCTGCCATGGAATCCCCTACTC; TACTGTGTGTAGCCTTACTGTTCTTCGCTCCAGATGGCGTGTTAGCAGTCCCTCAGAAAC; CTAAGGTCTCCTTGAACCCTCCATGGAATAGAATATTTAAAGGAGAGAATGTGACTCTTA; CATGTAATGGGAACAATTTCTTTGAAGTCAGTTCCACCAAATGGTTCCACAATGGCAGCC; TTTCAGAAGAGACAAATTCAAGTTTGAATATTGTGAATGCCAAATTTGAAGACAGTGGAG; AATACAAATGTCAGCACCAACAAGTTAATGAGAGTGAACCTGTGTACCTGGAAGTCTTCA; GTGACTGGCTGCTCCTTCAAGCCTCTGCTGAGGTGGTGATGGAGGGCCAGCCCCTCTTCC; TCAGGTGCCATGGTTGGAGGAACTGGGATGTGTACAAAGTGATCTATTATAAGGATGGTG; AAGCTCTCAAGTACTGGTATGAGAACCACAACATCTCCATTACAAATGCCACAGTTGAAG; ACAGTGGAACCTACTACTGTACGGGCAAAGTGTGGCAGCTGGACTATGAGTCTGAGCCCC; TCAACATTACTGTAATAAAAGCTCCGCGTGAGAAGTACTGGCTACAATTTTTTATCCCAT; TGTTGGTGGTGATTCTGTTTGCTGTGGACACAGGATTATTTATCTCAACTCAGCAGCAAG; TCACATTTCTCTTGAAGATTAAGAGAACAGGAAAGGCTTCAGACTTCTGAACCCCACATC; CTAGCCAAACCCCAAAACCACTG;
• Restriction Sites: Please inquire
• ACCN: NM_002001
• Insert Size: 1102 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002001.2, NP_001992.1
• RefSeq Size: 1191 bp
• RefSeq ORF: 774 bp
• Locus ID: 2205
• UniProt ID: P12319
• Cytogenetics: 1q23.2
• Protein Families: Transmembrane
• Protein Pathways: Asthma, Fc epsilon RI signaling pathway
• Summary: The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]