IL1RA (IL1RN) (NM_000577) Human Untagged Clone

SKU SC122567

IL1RN (untagged)-Human interleukin 1 receptor antagonist (IL1RN), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: IL1RA
• Synonyms: DIRA; ICIL-1RA; IL-1ra; IL-1ra3; IL-1RN; IL1F3; IL1RA; IRAP; MVCD4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000577 edited; GGAAGACCTCCTGTCCTATCAGGCCCTCCCCATGGCTTTAGAGACGATCTGCCGACCCTC; TGGGAGAAAATCCAGCAAGATGCAAGCCTTCAGAATCTGGGATGTTAACCAGAAGACCTT; CTATCTGAGGAACAACCAACTAGTTGCCGGATACTTGCAAGGACCAAATGTCAATTTAGA; AGAAAAGATAGATGTGGTACCCATTGAGCCTCATGCTCTGTTCTTGGGAATCCATGGAGG; GAAGATGTGCCTGTCCTGTGTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCAGT; TAACATCACTGACCTGAGCGAGAACAGAAAGCAGGACAAGCGCTTCGCCTTCATCCGCTC; AGACAGTGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTGCAC; AGCGATGGAAGCTGACCAGCCCGTCAGCCTCACCAATATGCCTGACGAAGGCGTCATGGT; CACCAAATTCTACTTCCAGGAGGACGAGTAGTACTGCCCAGGCCTGCCTGTTCCCATTCT; TGCATGGCAAGGACTGCAGGGACTGCCAGTCCCCCTGCCCCAGGGCTCCCGGCTATGGGG; GCACTGAGGACCAGCCATTGAGGGGTGGACCCTCAGAAGGCGTCACAACAACCTGGTCAC; AGGACTCTGCCTCCTCTTCAACTGACCAGCCTCCATGCTGCCTCCAGAATGGTCTTTCTA; ATGTGTGAATCAGAGCACAGCAGCCCCTGCACAAAGCCCTTCCATGTCGCCTCTGCATTC; AGGATCAAACCCCGACCACCTGCCCAACCTGCTCTCCTCTTGCCACTGCCTCTTCCTCCC; TCATTCCACCTTCCCATGCCCTGGATCCATCAGGCCACTTGATGACCCCCAACCAAGTGG; CTCCCACACCCTGTTTTACAAAAAAGAAAAGACCAGTCCATGAGGGAGGTTTTTAAGGGT; TTGTGGAAAATGAAAATTAGGATTTCATGATTTTTTTTTTTCAGTCCCCGTGAAGGAGAG; CCCTTCATTTGGAGATTATGTTCTTTCGGGGAGAGGCTGAGGACTTAAAATATTCCTGCA; TTTGTGAAATGATGGTGAAAGTAAGTGGTAGCTTTTCCCTTCTTTTTCTTCTTTTTTTGT; GATGTCCCAACTTGTAAAAATTAAAAGTTATGGTACTATGTTAGCCCCATAATTTTTTTT; TTCCTTTTAAAACACTTCCATAATCTGGACTCCTCTGTCCAGGCACTGCTGCCCAGCCTC; CAAGCTCCATCTCCACTCCAGATTTTTTACAGCTGCCTGCAGTACTTTACCTCCTATCAG; AAGTTTCTCAGCTCCCAAGGCTCTGAGCAAATGTGGCTCCTGGGGGTTCTTTCTTCCTCT; GCTGAAGGAATAAATTGCTCCTTGACATTGTAGAGCTTCTGGCACTTGGAGACTTGTATG; AAAGATGGCTGTGCCTCTGCCTGTCTCCCCCACCAGGCTGGGAGCTCTGCAGAGCAGGAA; ACATGACTCGTATATGTCTCAGGTCCCTGCAGGGCCAAGCACCTAGCCTCGCTCTTGGCA; GGTACTCAGCGAATGAATGCTGTATATGTTGGGTGCAAAGTTCCCTACTTCCTGTGACTT; CAGCTCTGTTTTACAATAAAATCTTGAAAATGCCTAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000577 unedited; ATAGCGTTCGCTTTTGTATACGACTCATTTAGGGCGGCCGCGTAATTCGCACGAGGGGGA; ANACCTCCTGTCCTATCAGGCCCTCCCCTGGCTTTAGAGACGATCTGCCGACCCTCTGGG; CAGAAAATCCAGCAAGATGCAAGCCTTCATAATCTGGGATGTTAACCAGAAGACCTTCTA; TCTGAGGAACAACCAACTAGTTGCCGGATACTTGCAAGGACCAAATGTCAATTTAGAAGA; AAAGATAGATGTGGTACCCATTGAGCCTCATGCTCTGTTCTTGGGAATCCATGGAGGGAA; GATGTGCCTGTCCTGTGTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCAGTTAA; CATCACTGACCTGAGCGAGAACAGAAAGCAGGACAAGCGCTTCGCCTTCATCCGCTCAGA; CAGTGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTGCACAGC; GATGGAAGCTGACCAGCCCGTCAGCCTCACCAATATGCCTGACGAATGCGTCATGGTCAC; CAAATTCTACTTCCAGGAGGACGAGTAGTACTGCCCAGGCCTGCCTGTTCCCATTCTTGC; ATGGCAAGGACTGCAGGGACTGCCAGTCCCCCTGCCCCAGGGCTCCCGGCTATGGGGGCA; CTGAGGACCCGCCATTGAGGGGTGGACCCTCAGAAGGCGTCACAACAACCTGGTCACAGG; ACTCTGCCTCCCCTTCAACTGACCAGCCTCCTTGCTGCCTACAGAATGGTCTTTCTAATG; TGTGAATCACACCCAGCATCCCCTTGAAAAGGCCTTTCCAGGTCGCTTTGGCTTTATGAT; CAAACCCCGGACCACTGGCCAACCGCTTTTCTCTTGGCATTGGCCCTTCCTTCCCTCATC; CGACCTGGCAG;
• Restriction Sites: Please inquire
• ACCN: NM_000577
• Insert Size: 1716 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000577.3, NP_000568.1
• RefSeq Size: 1802 bp
• RefSeq ORF: 480 bp
• Locus ID: 3557
• UniProt ID: P18510
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome, Secreted Protein
• Summary: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]; Transcript Variant: This variant (3) lacks an inframe coding segment, as compared to variant 2. The resulting isoform (3) lacks an internal region, as compared to the longest isoform (2).