HSD11B2 (NM_000196) Human Untagged Clone

CAT#: SC122552

HSD11B2 (untagged)-Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD11B2
• Synonyms: AME; AME1; HSD2; HSD11K; SDR9C3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000196 edited
  CCCTCTCGCGCCCCAGGCCGGTGTACCCCCGCACTCCGCGCCCCGGCCTAGAAGCTCTCT
  CTCCCCGCTCCCCGGCCCGGCCCCCGCCCCGCCCCGCCCCAGCCCGCTGGGCCGCCATGG
  AGCGCTGGCCTTGGCCGTCGGGCGGCGCCTGGCTGCTCGTGGCTGCCCGCGCGCTGCTGC
  AGCTGCTGCGCTCAGACCTGCGTCTGGGCCGCCCGCTGCTGGCGGCGCTGGCGCTGCTGG
  CCGCGCTCGACTGGCTGTGCCAGCGCCTGCTGCCCCCGCCGGCCGCACTCGCCGTGCTGG
  CCGCCGCCGGCTGGATCGCGTTGTCCCGCCTGGCGCGCCCGCAGCGCCTGCCGGTGGCCA
  CTCGCGCGGTGCTCATCACCGGCTGTGACTCTGGTTTTGGCAAGGAGACGGCCAAGAAAC
  TGGACTCCATGGGCTTCACGGTGCTGGCCACCGTATTGGAGTTGAACAGCCCCGGTGCCA
  TCGAGCTGCGTACCTGCTGCTCCCCTCGCCTAAGGCTGCTGCAGATGGACCTGACCAAAC
  CAGGAGACATTAGCCGCGTGCTAGAGTTCACCAAGGCCCACACCACCAGCACCGGCCTGT
  GGGGCCTCGTCAACAACGCAGGCCACAATGAAGTAGTTGCTGATGCGGAGCTGTCTCCAG
  TGGCCACTTTCCGTAGCTGCATGGAGGTGAATTTCTTTGGCGCGCTCGAGCTGACCAAGG
  GCCTCCTGCCCCTGCTGCGCAGCTCAAGGGGCCGCATCGTGACTGTGGGGAGCCCAGCGG
  GGGACATGCCATATCCGTGCTTGGGGGCCTATGGAACCTCCAAAGCGGCCGTGGCGCTAC
  TCATGGACACATTCAGCTGTGAACTCCTTCCCTGGGGGGTCAAGGTCAGCATCATCCAGC
  CTGGCTGCTTCAAGACAGAGTCAGTGAGAAACGTGGGTCAGTGGGAAAAGCGCAAGCAAT
  TGCTGCTGGCCAACCTGCCTCAAGAGCTGCTGCAGGCCTACGGCAAGGACTACATCGAGC
  ACTTGCATGGGCAGTTCCTGCACTCGCTACGCCTGGCCATGTCCGACCTCACCCCAGTTG
  TAGATGCCATCACAGATGCGCTGCTGGCAGCTCGGCCCCGCCGCCGCTATTACCCCGGCC
  AGGGCCTGGGGCTCATGTACTTCATCCACTACTACCTGCCTGAAGGCCTGCGGCGCCGCT
  TCCTGCAGGCCTTCTTCATCAGTCACTGTCTGCCTCGAGCACTGCAGCCTGGCCAGCCTG
  GCACTACCCCACCACAGGACGCAGCCCAGGGCCCAAACCTGAGCCCCGGCCCTTCCCCAG
  CAGTGGCTCGGTGAGCCATGTGCACCTATGGCCCAGCCACTGCAGCACAGGAGGCTCCGT
  GAGCCCTTGGTTCCTCCCCGAAAACCCCCAGCATTACGATCCCCCAAGTGTCCTGGACCC
  TGGCCTAAAGAATCCCACCCCCACTTCATGCCCACTGCCGATGCCCAATCCAGGCCCGGT
  GAGGCCAAGGTTTCCCAGTGAGCCTCTGCGCCTCTCCACTGTTTCATGAGCCCAAACACC
  CTCCTGGCACAACGCTCTACCCTGCAGCTTGGAGAACTCCGCTGGATGGGGAGTCTCATG
  CAAGACTTCACTGCAGCCTTTCACAGGACTCTGCAGATAGTGCCTCTGCAAACTAAGGAG
  TGACTAGGTGGGTTGGGGACCCCCTCAGGATTGTTTCTCGGCACCAGTGCCTCAGTGCTG
  CAATTGAGGGCTAAATCCCAAGTGTCTCTTGACTGGCTCAAGAATTAGGGCCCCAACTAC
  ACACCCCCAAGCCACAGGGAAGCATGTACTGTACTTCCCAATTGCCACATTTTAAATAAA
  GACAAATTTTTATTTCTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000196 unedited
  GGGTTTGGATTTGTATACGACTCATATAGGCGGCCGCGACTTCCCGGGATCCCTCTCGCG
  CCCCAGGCCGGTGTACCCCCGCACTCCGCGCCCCGGCCTAGAAGCTCTCTCTCCCCGCTC
  CCCGGCCCGGCCCCCGCCCCGCCCCGCCCCAGCCCGCTGGGCCGCCATGGAGCGCTGGCC
  TTGGCCGTCGGGCGGCGCCTGGCTGCTCGTGGCTGCCCGCGCGCTGCTGCAGCTGCTGCG
  CTCAGACCTGCGTCTGGGCCGCCCGCTGCTGGCGGCGCTGGCGCTGCTGGCCGCGCTCGA
  CTGGCTGTGCCAGCGCCTGCTGCCCCCGCCGGCCGCACTCGCCGTGCTGGCCGCCGCCGG
  CTGGATCGCGTTGTCCCGCCTGGCGCGCCCGCAGCGCCTGCCGGTGGCCACTCGCGCGGT
  GCTCATCACCGGCTGTGACTCTGGTTTTGGCAAGGAGACGGCCAAGAAACTGGACTCCAT
  GGGCTTCACGGTGCTGGCCACCGTATTGGAGTTGAACAGCCCCGGTGCCATCGAGCTGCG
  TACCTGCTGCTCCCCTCGCCTAAGGCTGCTGCAGATGGACCTGACCAAACCAGGAGACAT
  TAGCCGCGTGCTAGAGTTCACCAAGGCCCACACCACCAGCACCGGCCTGTGGGGCCTCGT
  CAACAACGCAGGCCACAATGAAGTAGTTGCTGATGCGGAGCTGTCTCCCATGGCCACTTT
  CCGTAGCTGCATGGAGGTGAATTTCTTTGGCGCGCTCGAGCTGACCCAAGGCCTCCTGCC
  TCTGCTGCGCAGCTAAAGGGCCGCATCGTGACTGTGGGGAGCCCACCGGGGGACATGCCA
  TATCCGTGCTTGGGGGCCCAATGGAACCTCCAAAGGGCGCGTGGCCCTCCTCATGGACAC
  ATTCGCCTGTGAACTCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_000196
• Insert Size: 1944 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000196.2, NP_000187.2
• RefSeq Size: 1944 bp
• RefSeq ORF: 1218 bp
• Locus ID: 3291
• UniProt ID: P80365
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]