CHRNA5 (NM_000745) Human Untagged Clone

SKU SC122164

CHRNA5 (untagged)-Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CHRNA5
• Synonyms: LNCR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC122164 sequence for NM_000745 edited (data generated by NextGen Sequencing); ATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTC; GCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTATCTGAACCT; TCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGACTACGAAAGA; TGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGACTTGCAATA; TCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTCTGGTTGAAA; CAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGAATAAAAGTT; ATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGATAATGCAGAT; GGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACTGTCACCTGG; ACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTCCCATTTGAC; CTTCAGAACTGTTCCATGAAATTTGGTTCTTGGACTTATGATGGATCACAGGTTGATATA; ATTCTAGAGGACCAAGATGTAGACAAGAGAGATTTTTTTGATAATGGAGAATGGGAGATT; GTGAGTGCAACAGGGAGCAAAGGAAACAGAACCGACAGCTGTTGCTGGTATCCGTATGTC; ACTTACTCATTTGTAATCAAGCGCCTGCCTCTCTTTTATACCTTGTTCCTTATAATACCC; TGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAATGAAGGTGAA; AAGATTTGTCTCTGCACTTCAGTACTTGTGTCTTTGACTGTCTTCCTTCTGGTTATTGAA; GAGATCATACCATCATCTTCAAAAGTCATACCTCTAATTGGAGAGTATCTGGTATTTACC; ATGATTTTTGTGACACTGTCAATTATGGTAACCGTCTTCGCTATCAACATTCATCATCGT; TCTTCCTCAACACATAATGCCATGGCGCCTTTGGTCCGCAAGATATTTCTTCACACGCTT; CCCAAACTGCTTTGCATGAGAAGTCATGTAGACAGGTACTTCACTCAGAAAGAGGAAACT; GAGAGTGGTAGTGGACCAAAATCTTCTAGAAACACATTGGAAGCTGCGCTCGATTCTATT; CGCTACATTACAAGACACATCATGAAGGAAAATGATGTCCGTGAGGTTGTTGAAGATTGG; AAATTCATAGCCCAGGTTCTTGATCGGATGTTTCTGTGGACTTTTCTTTTCGTTTCAATT; GTTGGATCTCTTGGGCTTTTTGTTCCTGTTATTTATAAATGGGCAAATATATTAATACCA; GTTCAAATTGGAAATGCAAATAAGTGA; ; Clone variation with respect to NM_000745.3; 1386 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000745 unedited; TATAGGCGGCCGCGAATTCGGCACGAGGCTGCTGTCCCGGCGGGAGCTGTGGCGCGGAGC; GGCCCCTCTGCTGCGTCTGCCCTCGTTTTGTCTCACGACTCACACTCAGTGCTCCATTCC; CCAAGAGTTCGCGTTCCCCGCGCGGCGGTCGAGAGGCGGCTGCCCGCGGTCCCGCGCGGG; CGCGGGGCGATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTC; CAGCTGGTCGCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTA; TCTGAACCTTCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGAC; TACGAAAGATGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGA; CTTGCAATATCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTC; TGGTTGAAACAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGA; ATAAAAGTTATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGAT; AATGCAGATGGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACT; GTCACCTGGACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTT; CCATTTGACCTTCAGAACTGTTCCATGANATTTGGTTCTTGGACTTATGATGGATCACAG; GTTGATATATTNCTAGAGGACAAGATGTAGACCAGAGAGATTTTTTTGATATGGAGATGG; GNAGATTGTGAGTGCACAGGGAGCAAAGGAACAGACCGNCAGCTGTGGCTGGTTTCGNAT; GTCCT;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000745 unedited; AATTGGGGCCTTAGTTCCGGTTTATTTAACCCAGGTTCAGGAATATTAATACCAGTCTCA; AATTGGNAAATGCAAATAATTGAAGCCTCCCAGGGNACTGAAGTATATACATTTAGTTAA; CACACATATATCTGATGGCACCTATAAAATTATGAAAATGTAAGTTATGTGTTAAATTTA; GTGCAAGCTTTAACAGACTAAGTTGCTAACCTCAATTTATGTTAACAGATTATCCATTTG; AACAGTTGGCTGTATGACTGAAGTAATAACTGATGAGATACATTTGATCTTGTAAAAATA; GCAAAATATTATCTGAACTGGACTAGTGAAAAATCTAGTATTTGTATCCTGGCAAATAAT; ACTAATTTATAATCCACAGTAAAGTTCATCCTTTGACTGTGCTGGAGAATTCCAGTTGTA; TTTGAAGACTGATTTTAAAACTTTTCTGCATTTGGTAAAGGTATGTAAACTTTTCTGCAC; TCACTGAGTAACAGCTAATCTTTATATCATATTATACTGTTATATTTTGAAAAACTGACT; ACCTGATATAATTCCTTATTGCGATGTTTGGTATAATGACTACTTATTGTGTCCTGCTTC; CGGTGGCCTACACCTGTTATCTCGGTGTGTTGACGTTACAGACTTCCTCGGGTAACCATA; GGGGCGCGAACGCAAGGAGCCACAGCCGGGGTAGCTATGGCATATGATTGTGTTATGCCA; GTAACTATCCTAGGTCGTTAACTTTTTATGCCAGCCATTTATATGCCCGGACCCCTGTTG; CAACAATTTTTCCAACAGTCTTCCTATTACTTGTCAATAGGGGCGGGAGTACAGCGGGTC; GGTCTGGCCGCCTATTTCCCCCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000745
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000745.2, NP_000736.2
• RefSeq Size: 2468 bp
• RefSeq ORF: 1407 bp
• Locus ID: 1138
• UniProt ID: P30532
• Cytogenetics: 15q25.1
• Domains: Neur_chan_LBD, Neur_chan_memb
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Summary: The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010]; Transcript Variant: his variant (1) represents the longer transcript and encodes the longer isoform (1).