GJD2 (NM_020660) Human Untagged Clone

CAT#: SC122104

GJD2 (untagged)-Human gap junction protein, delta 2, 36kDa (GJD2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GJD2
• Synonyms: CX36; GJA9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC122104 sequence for NM_020660 edited (data generated by NextGen Sequencing)
  ATGGGGGAATGGACCATCTTGGAGAGGCTGCTAGAAGCCGCGGTGCAGCAGCACTCCACT
  ATGATCGGGAGGATCCTGTTGACTGTGGTGGTGATCTTCCGGATCCTCATTGTGGCCATT
  GTGGGGGAGACGGTGTACGATGATGAGCAGACCATGTTTGTGTGCAACACCCTGCAGCCC
  GGCTGTAACCAGGCCTGCTATGACCGCGCCTTCCCCATCTCCCACATACGTTACTGGGTC
  TTCCAGATCATAATGGTGTGTACCCCCAGTCTTTGCTTCATCACCTACTCTGTGCACCAG
  TCCGCCAAGCAGCGAGAACGCCGCTACTCTACAGTCTTCCTAGCCCTGGACAGAGACCCC
  CCTGAGTCCATAGGAGGTCCTGGAGGAACTGGGGGTGGGGGCAGTGGTGGGGGCAAACGA
  GAAGATAAGAAGTTGCAAAATGCTATTGTGAATGGGGTGCTGCAGAACACAGAGAACACC
  AGTAAGGAGACAGAGCCAGATTGTTTAGAGGTTAAGGAGCTGACTCCACACCCATCAGGT
  CTACGCACTGCATCAAAATCCAAGCTCAGAAGGCAGGAAGGCATCTCCCGCTTCTACATT
  ATCCAAGTGGTGTTCCGAAATGCCCTGGAAATTGGGTTCCTGGTTGGCCAATATTTTCTC
  TATGGCTTTAGTGTCCCAGGGTTGTATGAGTGTAACCGCTACCCCTGCATCAAGGAGGTG
  GAATGTTATGTGTCCCGGCCAACTGAGAAGACTGTCTTTCTAGTGTTCATGTTTGCTGTA
  AGTGGCATCTGTGTTGTGCTCAACCTGGCTGAACTCAACCACCTGGGATGGCGCAAGATC
  AAGCTGGCTGTGCGAGGGGCTCAGGCCAAGAGAAAGTCAATCTATGAGATTCGTAACAAG
  GACCTGCCAAGGGTCAGTGTTCCCAATTTTGGCAGGACTCAGTCCAGTGACTCTGCCTAT
  GTGTGA
  
  Clone variation with respect to NM_020660.1
  69 a=>g;207 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020660 unedited
  NNNGGTTCGNAATTTTGTAATACGACTCACTATAGGGCGGCCGCGATTCGCCCTTTGGGG
  GAATGGACCATCTTGGAGAGGCTGCTAGAAGCCGCGGTGCAGCAGCACTCCACTATGATC
  GGGAGGATCCTGTTGACTGTGGTGGTGATCTTCCGGATCCTCATTGTGGCCATTGTGGGG
  GAGACGGTGTACGATGATGAGCAGACCATGTTTGTGTGCAACACCCTGCAGCCCGGCTGT
  AACCAGGCCTGCTATGACCGCGCCTTCCCCATCTCCCACATACGTTACTGGGTCTTCCAG
  ATCATAATGGTGTGTACCCCCAGTCTTTGCTTCATCACCTACTCTGTGCACCAGTCCGCC
  AAGCAGCGAGAACGCCGCTACTCTACAGTCTTCCTAGCCCTGGACAGAGACCCCCCTGAG
  TCCATAGGAGGTCCTGGAGGAACTGGGGGTGGGGGCAGTGGTGGGGGCAAACGAGAAGAT
  AAGAAGTTGCAAAATGCTATTGTGAATGGGGTGCTGCAGAACACAGAGAACACCAGTAAG
  GAGACAGAGCCAGATTGTTTAGAGGTTAAGGAGCTGACTCCACACCCATCAGGTCTACGC
  ACTGCATCAAAATCCAAGCTCAGAAGGCAGGAAGGCATCTCCCGCTTCTACATTATCCAA
  GTGGTGTTCCGAAATGCCCTGGAAATTGGGTTCCTGGTTGGCCAATATTTTCTCTATGGC
  TTTAGTGTCCCAGGGTTGTATGAGTGTAACCGCTACCCCTGCATCAAGGAGGTGGAATGT
  TATGTGTCCCGGCCAACTGAGAAGACTGTCTTTCTAGTGTTCATGTTTGCTGNTAGTGGC
  ATCTGTGTTGTGCTCAACCTGGCTGAACTCACCACCTGGGATGGCGCAAGATCAAGCTGG
  CTGTGCGAGGGGCTCAGGCCAGAGAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_020660
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020660.1, NP_065711.1
• RefSeq Size: 966 bp
• RefSeq ORF: 966 bp
• Locus ID: 57369
• UniProt ID: Q9UKL4
• Cytogenetics: 15q14
• Protein Families: Ion Channels: Other, Transmembrane
• Protein Pathways: Gap junction
• Gene Summary: This gene encodes a member of the connexin protein family. Connexins are gap junction proteins which are arranged in groups of 6 around a central pore to form a connexon, a component of the gap junction intercellular channel. The channels formed by this protein allow cationic molecule exchange between human beta cells and may function in the regulation of insulin secretion. [provided by RefSeq, Oct 2012]