Aquaporin 1 (AQP1) (NM_198098) Human Untagged Clone

SKU SC121943

AQP1 (untagged)-Human aquaporin 1 (Colton blood group) (AQP1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Aquaporin 1
• Synonyms: AQP-CHIP; CHIP28; CO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121943 sequence for NM_198098 edited (data generated by NextGen Sequencing); ATGGCCAGCGAGTTCAAGAAGAAGCTCTTCTGGAGGGCAGTGGTGGCCGAGTTCCTGGCC; ACGACCCTCTTTGTCTTCATCAGCATCGGTTCTGCCCTGGGCTTCAAATACCCGGTGGGG; AACAACCAGACGGCGGTCCAGGACAACGTGAAGGTGTCGCTGGCCTTCGGGCTGAGCATC; GCCACGCTGGCGCAGAGTGTGGGCCACATCAGCGGCGCCCACCTCAACCCGGCTGTCACA; CTGGGGCTGCTGCTCAGCTGCCAGATCAGCATCTTCCGTGCCCTCATGTACATCATCGCC; CAGTGCGTGGGGGCCATCGTCGCCACCGCCATCCTCTCAGGCATCACCTCCTCCCTGACT; GGGAACTCGCTTGGCCGCAATGACCTGGCTGATGGTGTGAACTCGGGCCAGGGCCTGGGC; ATCGAGATCATCGGGACCCTCCAGCTGGTGCTATGCGTGCTGGCTACTACCGACCGGAGG; CGCCGTGACCTTGGTGGCTCAGCCCCCCTTGCCATCGGCCTCTCTGTAGCCCTTGGACAC; CTCCTGGCTATTGACTACACTGGCTGTGGGATTAACCCTGCTCGGTCCTTTGGCTCCGCG; GTGATCACACACAACTTCAGCAACCACTGGATTTTCTGGGTGGGGCCATTCATCGGGGGA; GCCCTGGCTGTACTCATCTACGACTTCATCCTGGCCCCACGCAGCAGTGACCTCACAGAC; CGCGTGAAGGTGTGGACCAGCGGCCAGGTGGAGGAGTATGACCTGGATGCCGACGACATC; AACTCCAGGGTGGAGATGAAGCCCAAATAG; ; Clone variation with respect to NM_198098.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198098 unedited; TCAAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACCAGCTCAGGCCAAGCC; CCCTGCCAGCATGGCCAGCGAGTTCAAGAAGAAGCTCTTCTGGAGGGCAGTGGTGGCCGA; GTTCCTGGCCACGACCCTCTTTGTCTTCATCAGCATCGGTTCTGCCCTGGGCTTCAAATA; CCCGGTGGGGAACAACCAGACGGCGGTCCAGGACAACGTGAAGGTGTCGCTGGCCTTCGG; GCTGAGCATCGCCACGCTGGCGCAGAGTGTGGGCCACATCAGCGGCGCCCACCTCAACCC; GGCTGTCACACTGGGGCTGCTGCTCAGCTGCCAGATCAGCATCTTCCGTGCCCTCATGTA; CATCATCGCCCAGTGCGTGGGGGCCATCGTCGCCACCGCCATCCTCTCAGGCATCACCTC; CTCCCTGACTGGGAACTCGCTTGGCCGCAATGACCTGGCTGATGGTGTGAACTCGGGCCA; GGGCCTGGGCATCGAGATCATCGGGACCCTCCAGCTGGTGCTATGCGTGCTGGCTACTAC; CGACCGGAGGCGCCGTGACCTTGGTGGCTCAGCCCCCCTTGCCATCGGCCTCTCTGTAGC; CCTTGGACACCTCCTGGCTATTGACTACACTGGCTGTGGGATTAACCCTGCTCGGTCCTT; TGGCTCCGCGGTGATCACACACAACTTCAGCAACCACTGGATNNTCTGGGTGGNGCCATT; CATCGNGGGAGCCCTGGCTGTACTCATCTACGACTTCATCCTGGCCCCACGCAGCAGTGA; CCTCACAGAACGCGTGAANGGTGTNGACCANNCGCCCAGTGGNAAGGAGTATGACCCTGG; ATGCCNGACGACATCCACTCCCAGGGTGGNAAATGAAGCCCATANAAGAGGGGTCTGGCC; CGGCATCCACCTNNGGGGAGGCAGNCCCNCCCCCCCAGANNNNNNNNNNNNNNGGGTTAA; ACT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_198098 unedited; TTTTTTTTTTTTTTTTTGACCTTACAAGGGATTTTCCTTTATTTTCTTAACATATAATGA; AATTCCAACTCCAAAAAGACATATTTGCAGAGCAAACAGATGCAGGGCCTTGGGGTGCAA; CAACCTAAAACCAGGCAGGGGGGGGGGGGGACCCCTTTCCCCTTCATTACAACTTTTCCC; ACTCCTGGCCCCTGGCCATTGCCCAGGCAAAAACTGAAAAGCTGGAAATGAAAGGAATCA; ACCTCGTGGTCCAGGGAGTAACCAAAAACAGGGCCTTGGTTACTAGGCCTGGCCAAATCA; TTGTCTAACTGAAACCGGGGGCCTCAGTTTCTTTATCTGTTAAATGAGCTAAAAGTTCCT; GCTAGGGAATGGAAAAAAGAGTGTGCAAATGTGATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_198098
• Insert Size: 810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198098.1, NP_932766.1
• RefSeq Size: 2764 bp
• RefSeq ORF: 810 bp
• Locus ID: 358
• UniProt ID: P29972
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Summary: This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq, Aug 2016]; Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.