Oncostatin M (OSM) (NM_020530) Human Untagged Clone

SKU SC121421

OSM (untagged)-Human oncostatin M (OSM)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Oncostatin M
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020530 edited; GTCACCCCCAGCGGGCGCGGGCCGGAGCACGGGCACCCAGCATGGGGGTACTGCTCACAC; AGAGGACGCTGCTCAGTCTGGTCCTTGCACTCCTGTTTCCAAGCATGGCGAGCATGGCGG; CTATAGGCAGCTGCTCGAAAGAGTACCGCGTGCTCCTTGGCCAGCTCCAGAAGCAGACAG; ATCTCATGCAGGACACCAGCAGACTCCTGGACCCCTATATACGTATCCAAGGCCTGGATG; TTCCTAAACTGAGAGAGCACTGCAGGGAGCGCCCCGGGGCCTTCCCCAGTGAGGAGACCC; TGAGGGGGCTGGGCAGGCGGGGCTTCCTGCAGACCCTCAATGCCACACTGGGCTGCGTCC; TGCACAGACTGGCCGACTTAGAGCAGCGCCTCCCCAAGGCCCAGGATTTGGAGAGGTCTG; GGCTGAACATCGAGGACTTGGAGAAGCTGCAGATGGCGAGGCCGAACATCCTCGGGCTCA; GGAACAACATCTACTGCATGGCCCAGCTGCTGGACAACTCAGACACGGCTGAGCCCACGA; AGGCTGGCCGGGGGGCCTCTCAGCCGCCCACCCCCACCCCTGCCTCGGATGCTTTTCAGC; GCAAGCTGGAGGGCTGCAGGTTCCTGCATGGCTACCATCGCTTCATGCACTCAGTGGGGC; GGGTCTTCAGCAAGTGGGGGGAGAGCCCGAACCGGAGCCGGAGACACAGCCCCCACCAGG; CCCTGAGGAAGGGGGTGCGCAGGACCAGACCCTCCAGGAAAGGCAAGAGACTCATGACCA; GGGGACAGCTGCCCCGGTAGCCTCGAGAGCACCCCTTGCCGGTGAAGGATGCGGCAGGTG; CTCTGTGGATGAGAGGAACCATCGCAGGATGACAGCTCCCGGGTCCCCAAACCTGTTCCC; CTCTGCTACTAGCCACTGAGAAGTGCACTTTAAGAGGTGGGAGCTGGGCAGACCCCTCTA; CCTCCTCCAGGCTGGGAGACAGAGTCAGGCTGTTGCGCTCCCACCTCAGCCCCAAGTTCC; CCAGGCCCAGTGGGGTGGCCGGGCGGGCCACGCGGGACCGACTTTCCATTGATTCAGGGG; TCTGATGACACAGGCTGACTCATGGCCGGGCTGACTGCCCCCCTGCCTTGCTCCCCGAGG; CCTGCCGGTCCTTCCCTCTCATGACTTGCAGGGCCGTTGCCCCCAGACTTCCTCCTTTCC; GTGTTTCTGAAGGGGAGGTCACAGCCTGAGCTGGCCTCCTATGCCTCATCATGTCCCAAA; CCAGACACCTGGATGTCTGGGTGACCTCACTTTAGGCAGCTGTAACAGCGGCAGGGTGTC; CCAGGAGCCCTGATCCGGGGGTCCAGGGAATGGAGCTCAGGTCCCAGGCCAGCCCCGAAG; TCGCCACGTGGCCTGGGGCAGGTCACTTTACCTCTGTGGACCTGTTTTCTCTTTGTGAAG; CTAGGGAGTTAGAGGCTGTACAAGGCCCCCACTGCCTGTCGGTTGCTTGGATTCCCTGAC; GTAAGGTGGATATTAAAAATCTGTAAATCAGGACAGGTGGTGCAAATGGCGCTGGGAGGT; GTACACGGAGGTCTCTGTAAAAGCAGACCCACCTCCCAGCGCCGGGAAGCCCGTCTTGGG; TCCTCGCTGCTGGCTGCTCCCCCTGGTGGTGGATCCTGGAATTTTCTCACGCAGGAGCCA; TTGCTCTCCTAGAGGGGGTCTCAGAAACTGCGAGGCCAGTTCCTTGGAGGGACATGACTA; ATTTATCGATTTTTATCAATTTTTATCAGTTTTATATTTATAAGCCTTATTTATGATGTA; TATTTAATGTTAATATTGTGCAAACTTATATTTAAAACTTGCCTGGTTTCTAAAAAAAAA; AAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020530 unedited; GGTCAAACATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCACCCCC; AGCGGGCGCGGGCCGNGACACGGGCACCCAGCATGGGGGTACTGCTCACACAGAGGACGC; TGCTCAGTCTGGTCCTTGCACTCCTGTTTCCAAGCATGGCGAGCATGGCGGCTATAGGCA; GCTGCTCGAAAGAGTACCGCGTGCTCCTTGGCCAGCTCCAGAAGCAGACAGATCTCATGC; AGGACACCAGCAGACTCCTGGACCCCTATATACGTATCCAAGGCCTGGATGTTCCTAAAC; TGAGAGAGCACTGCAGGGAGCGCCCCGGGGCCTTCCCCAGTGAGGAGACCCTGAGGGGGC; TGGGCAGGCGGGGCTTCCTGCAGACCCTCAATGCCACACTGGGCTGCGTCCTGCACAGAC; TGGCCGACTTAGAGCAGCGCCTCCCCAAGGCCCAGGATTTGGAGAGGTCTGGGCTGAACA; TCGAGGACTTGGAGAAGCTGCAGATGGCGAGGCCGAACATCCTCGGGCTCAGGAACAACA; TCTACTGCATGGCCCAGCTGCTGGACAACTCAGACACGGCTGAGCCCACGAAGGCTGGCC; GGGGGGCCTCTCAGCCGCCCACCCCCACCCCTGCCTCGGATGCTTTTCAGCGCAAGCTGG; ANGGCTGCAGGTTCCTGCATGGCTACCATCGCTTCATGCACTCAGTGGGGCGGGTCTTCA; GCAAGTGGGGGGAGAGCCCGACCCGGACCGGAGACACAGCCCCCACCAGGCCCTGAGGAA; GNGGGGTGCGCAGACCAGACCCTCCAGGAAAGGCAGAGACTCATGACCGGGGACAGCTGC; CCGGTAGCCTCGAGTTTTTTTTTTTTTTTTTCTTTGCCCGTTCCTTAATGGGGGAGGGCA; CAAGAAAAGCCTTCGCTCCCT;
• Restriction Sites: Please inquire
• ACCN: NM_020530
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020530.3, NP_065391.1
• RefSeq Size: 1880 bp
• RefSeq ORF: 759 bp
• Locus ID: 5008
• UniProt ID: P13725
• Cytogenetics: 22q12.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Summary: This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).