TRIF (TICAM1) (NM_182919) Human Untagged Clone

SKU SC120956

TICAM1 (untagged)-Human toll-like receptor adaptor molecule 1 (TICAM1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIF
• Synonyms: IIAE6; MyD88-3; PRVTIRB; TICAM-1; TRIF
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_182919 edited; GTGTGGAACATGCCTTCACCACCTCCAGCTTCTGCTGCCGGAGGCTGCACCCACCTGTGC; CCATGGCCTGCACGGGCCCATCACTTCCTAGCGCCTTCGACATTCTAGGTGCAGCAGGCC; AGGACAAGCTCTTGTATCTGAAGCACAAACTGAAGACCCCACGCCCAGGCTGCCAGGGGC; AGGACCTCCTGCATGCCATGGTTCTCCTGAAGCTGGGCCAGGAAACTGAGGCCAGGATCT; CTCTAGAGGCATTGAAGGCCGATGCGGTGGCCCGGCTGGTGGCCCGCCAGTGGGCTGGCG; TGGACAGCACCGAGGACCCAGAGGAGCCCCCAGATGTGTCCTGGGCTGTGGCCCGCTTGT; ACCACCTGCTGGCTGAGGAGAAGCTGTGCCCCGCCTCGCTGCGGGACGTGGCCTACCAGG; AAGCCGTCCGCACCCTCAGCTCCAGGGACGACCACCGGCTGGGGGAACTTCAGGATGAGG; CCCGAAACCGGTGTGGGTGGGACATTGCTGGGGATCCAGGGAGCATCCGGACGCTCCAGT; CCAATCTGGGCTGCCTCCCACCATCCTCGGCTTTGCCCTTTGGGACCAGGAGCCTCCCAC; GCCCCATTGACGGTGTTTCGGACTGGAGCCAAGGGTGCTCCCTGCGATCCACTGGCAGCC; CTGCCTCCCTGGCCAGCAACTTGGAAATCAGCCAGTCCCCTACCATGCCCTTCCTCAGCC; TGCACCGCAGCCCACATGGGCCCAGCAAGCTCTGTGACGACCCCCAGGCCAGCTTGGTGC; CCGAGCCTGTCCCCGGTGGCTGCCAGGAGCCTGAGGAGATGAGCTGGCCGCCATCGGGGG; AGATTGCCAGCCCACCAGAGCTGCCAAGCAGCCCACCTCCTGGGCTTCCCGAAGTGGCCC; CAGATGCAACCTCCACTGGCCTCCCTGATACCCCCGCAGCTCCAGAAACCAGCACCAACT; ACCCAGTGGAGTGCACCGAGGGGTCTGCAGGCCCCCAGTCTCTCCCCTTGCCTATTCTGG; AGCCGGTCAAAAACCCCTGCTCTGTCAAAGACCAGACGCCACTCCAACTTTCTGTAGAAG; ATACCACCTCTCCAAATACCAAGCCGTGCCCACCTACTCCCACCACCCCAGAAACATCCC; CTCCTCCTCCTCCTCCTCCTCCTTCATCTACTCCTTGTTCAGCTCACCTGACCCCCTCCT; CCCTGTTCCCTTCCTCCCTGGAATCATCATCGGAACAGAAATTCTATAACTTTGTGATCC; TCCACGCCAGGGCAGACGAACACATCGCCCTGCGGGTTCGGGAGAAGCTGGAGGCCCTTG; GCGTGCCCGACGGGGCCACCTTCTGCGAGGATTTCCAGGTGCCGGGGCGCGGGGAGCTGA; GCTGCCTGCAGGACGCCATAGACCACTCAGCTTTCATCATCCTACTTCTCACCTCCAACT; TCGACTGTCGCCTGAGCCTGCACCAGGTGAACCAAGCCATGATGAGCAACCTCACGCGAC; AGGGGTCGCCAGACTGTGTCATCCCCTTCCTGCCCCTGGAGAGCTCCCCGGCCCAGCTCA; GCTCCGACACGGCCAGCCTGCTCTCCGGGCTGGTGCGGCTGGACGAACACTCCCAGATCT; TCGCCAGGAAGGTGGCCAACACCTTCAAGCCCCACAGGCTTCAGGCCCGAAAGGCCATGT; GGAGGAAGGAACAGGACACCCGAGCCCTGCGGGAACAGAGCCAACACCTGGATGGTGAGC; GGATGCAGGCGGCGGCACTGAACGCAGCCTACTCAGCCTACCTCCAGAGCTACTTGTCCT; ACCAGGCACAGATGGAGCAGCTCCAGGTGGCTTTTGGGAGCCACATGTCATTTGGGACTG; GGGCGCCCTATGGGGCTCGAATGCCCTTTGGGGGCCAGGTGCCCCTGGGAGCCCCGCCAC; CCTTTCCCACTTGGCCGGGGTGCCCGCAGCCGCCACCCCTGCACGCATGGCAGGCTGGCA; CCCCCCCACCGCCCTCCCCACAGCCAGCAGCCTTTCCACAGTCACTGCCCTTCCCGCAGT; CCCCAGCCTTCCCTACGGCCTCACCCGCACCCCCTCAGAGCCCAGGGCTGCAACCCCTCA; TTATCCACCACGCACAGATGGTACAGCTGGGGCTGAACAACCACATGTGGAACCAGAGAG; GGTCCCAGGCGCCCGAGGACAAGACGCAGGAGGCAGAATGACCGCGTGTCCTTGCCTGAC; CACCTGGGGAACACCCCTGGACCCAGGCATCGGCCAGGACCCCATAGAGCACCCCGGTCT; GCCCTGTGCCCGGTGGACAGT;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182919 unedited; NCGCCATCCCCCGCCCCCCGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT; AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC; CGCGAATTCGGCACGAGGGCGGGGTGGAGCCAGCGCCCTCAGCGCGCTACGGTCCGCGGG; CAACTCCGCAGAAGCCCCAGCCCCCAGGACCCCAGGACCCAGTGGCGCAGCCGGCAGCCC; CGGATCCCTGATCTGCTTGGGCAGCTCCTGCAGAACCTGGAACAGTGAATGGGTAGGGGA; CACTGGGCGTGCAGAAGGCGGGGGGCAGTGTGGAACATGCCTTCACCACCTCCAGCTTCT; GCTGCCGGAGGCTGCACCCACCTGTGCCCATGGCCTGCACGGGCCCATCACTTCCTAGCG; CCTTCGACATTCTAGGTGCAGCAGGCCAGGACAAGCTCTTGTATCTGAAGCACAAACTGA; AGACCCCACGCCCAGGCTGCCAGGGGCAGGACCTCCTGCATGCCATGGTTCTCCTGAAGC; TGGGCCAGGAAACTGAGGCCAGGATCTCTCTAGAGGCATTGAAGGCCGATGCGGTGGCCC; GGCTGGTGGCCCGCCAGTGGGCTGGCGTGGACAGCACCGAGGACCCAGAGGAGCCCCCAG; ATGTGTCCTGGGCTGTGGCCCGCTTGTACCACCTGCTGGCTGAGGAGAAGCTGTGCCCCG; CCTCGCTGCGGGACGTGGCCTACCAGGAAGCCGTCCGCACCCTCAGCTCCAGGGACGACC; ACTCGCTGNGGGCACTTCANGATGANGCCCGAAACCTGTGTGGGGTGGACATTGCTGGNG; ATCCAGGGAGCATCCGGACGCTCCAGTNCATCTGGGCTGCCTCCCACATCCTNCGCTTTG; CCATTTGGN;
• Restriction Sites: NotI-NotI
• ACCN: NM_182919
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_182919.1, NP_891549.1
• RefSeq Size: 2460 bp
• RefSeq ORF: 2139 bp
• Locus ID: 148022
• UniProt ID: Q8IUC6
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Toll-like receptor signaling pathway
• Summary: This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. Mutations in this gene are associated with encephalopathy, acute, infection-induced. [provided by RefSeq, Jul 2020]