Cytochrome P450 2A6 (CYP2A6) (NM_000762) Human Untagged Clone

CAT#: SC120818

CYP2A6 (untagged)-Human cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome P450 2A6
• Synonyms: CPA6; CYP2A; CYP2A3; CYPIIA6; P450C2A; P450PB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120818 sequence for NM_000762 edited (data generated by NextGen Sequencing)
  ATGCTGGCCTCAGGGATGCTTCTGGTGGCCTTGCTGGTCTGCCTGACTGTGATGGTCTTG
  ATGTCTGTTTGGCAGCAGAGGAAGAGCAAGGGGAAGCTGCCTCCGGGACCCACCCCATTG
  CCCTTCATTGGAAACTACCTGCAGCTGAACACAGAGCAGATGTACAACTCCCTCATGAAG
  ATCAGTGAGCGCTATGGCCCCGTGTTCACCATTCACTTGGGGCCCCGGCGGGTCGTGGTG
  CTGTGTGGACATGATGCCGTCAGGGAGGCTCTGGTGGACCAGGCTGAGGAGTTCAGCGGG
  CGAGGCGAGCAAGCCACCTTCGACTGGGTCTTCAAAGGCTATGGCGTGGTATTCAGCAAC
  GGGGAGCGCGCCAAGCAGCTCCGGCGCTTCTCCATCGCCACCCTGCGGGACTTCGGGGTG
  GGCAAGCGAGGCATCGAGGAGCGCATCCAGGAGGAGGCGGGCTTCCTCATCGACGCCCTC
  CGGGGCACTGGCGGCGCCAATATCGATCCCACCTTCTTCCTGAGCCGCACAGTCTCCAAT
  GTCATCAGCTCCATTGTCTTTGGGGACCGCTTTGACTATAAGGACAAAGAGTTCCTGTCA
  CTGTTGCGCATGATGCTAGGAATCTTCCAGTTCACGTCAACCTCCACGGGGCAGCTCTAT
  GAGATGTTCTCTTCGGTGATGAAACACCTGCCAGGACCACAGCAACAGGCCTTTCAGTTG
  CTGCAAGGGCTGGAGGACTTCATAGCCAAGAAGGTGGAGCACAACCAGCGCACGCTGGAT
  CCCAATTCCCCACGGGACTTCATTGACTCCTTTCTCATCCGCATGCAGGAGGAGGAGAAG
  AACCCCAACACGGAGTTCTACTTGAAAAACCTGGTGATGACCACGTTGAACCTCTTCATT
  GGGGGCACCGAGACCGTCAGCACCACCCTGCGCTATGGCTTCTTGCTGCTCATGAAGCAC
  CCAGAGGTGGAGGCCAAGGTCCATGAGGAGATTGACAGAGTGATCGGCAAGAACCGGCAG
  CCCAAGTTTGAGGACCGGGCCAAGATGCCCTACATGGAGGCAGTGATCCACGAGATCCAA
  AGATTTGGAGACGTGATCCCCATGAGTTTGGCCCGCAGAGTCAAAAAGGACACCAAGTTT
  CGGGATTTCTTCCTCCCTAAGGGCACCGAAGTGTACCCTATGCTGGGCTCTGTGCTGAGA
  GACCCCAGTTTCTTCTCCAACCCCCAGGACTTCAATCCCCAGCACTTCCTGAATGAGAAG
  GGGCAGTTTAAGAAGAGTGATGCTTTTGTGCCCTTTTCCATCGGAAAGCGGAACTGTTTC
  GGAGAAGGCCTGGCCAGAATGGAGCTCTTTCTCTTCTTCACCACCGTCATGCAGAACTTC
  CGCCTCAAGTCCTCCCAGTCACCTAAGGACATTGACGTGTCCCCCAAACACGTGGGCTTT
  GCCACGATCCCACGAAACTACACCATGAGCTTCCTGCCCCGCTGA
  
  Clone variation with respect to NM_000762.5
  51 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000762 unedited
  AGGAATTTGTAATACGACTCACTATAGGCGGCCCGCGAATTCGCACCATCCACCATGCTG
  GCCTCAGGGATGCTTCTGGTGGCCTTGCTGGTCTGCCTGACTGTGATGGTCTTGATGTCT
  GTTTGGCAGCAGAGGAAGATCAAGGGGAAGCTGCCTCCGGGACCCACCCCATTGCCCTTC
  ATTGGAAACTACCTGCAGCTGAACACAGAGCAGATGTACAACTCCCTCATGAAGATCAGT
  GAGCGCTATGGCCCCGTGTTCACCATTCACTTGGGGCCCCGGCGGGTCGTGGTGCTGTGT
  GGACATGATGCCGTCAGGGAGGCTCTGGTGGACCATGCTGAAGAGTTCAGCGGGCGATGC
  GAGCAAGCCACCTTCGACTGGGTCTTCAAAGGCTATGGCGTGGTATTCAGCAACGGGGAG
  CGCGCCAAGCAGCTCCGGCGCTTCTCCATCGCCACCCTGCGGGACTTCGGGGTGGGCAAG
  CGAGGCATCGAGGAGCGCATCCAGGATGATGCGGGCTTCCTCATCGACGCCCTCCGGGGC
  ACTGGCGGCGCCAATATCGATCCCACCTTCTTCCTGAGCCGCACAGTCTCCAATGTCATC
  AGCTCCATTGTCTTTGGGGACCGCTTTGACTATAAAGACAAAGAGTTCCTGTCACTGTTG
  CGCATGATGCTAAGAATCTTCCAGTTCACGTCAACCTCCACGGAGCAGCTCTATGAGATG
  TTCTCTTCGGTGATGAAACACCTGCCATGACCACAGCATCAGGCCTTTCATTTGCTGCAA
  GGGCTGGATGACTTCATAGCCAAGAATGTGGAGCACAACCAGCGCACGCTGGATCCCANN
  TCCCACGGNACTCATTGACTCCTTCTATCGCTGCAGGAGGATGATATATCCCACACGNGT
  TCTACTGAAACCTGTGATGACACGCTGACTCTCATGGGGGCACGAGACGTAGCACACCTG
  GCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000762 unedited
  CGGATTGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCT
  ATGACCTTGGCCGCAATCTAGAGTCGAGTTTTTTTTTGGTTTTTTTTCAGGAAATAAGAG
  CTGCTATTATTACTACTCTTCATAGCATAATGTAAGGGTTTCCTTCCTCTCATCCCAGCT
  CGGAAGCACCTTATCAAGGTGAACTGAGCCCCTTCTGTTTCTTCTCTTCCCTCTAGCCAC
  CACGCCCCTTCCTTTCCCGCATCTTCCCCCCATTCTTATACCCGCCTCTTCCGCGAACCC
  CGCCCTGACCCCGCCTTTCCCTGGCCCCGCCCACCAGACCTGCACCGGCACAGCCCTCGC
  TCAGCGGGGCAGGAAGCTCATGGTGTAGTTTCGTGGGATCGTGGCAAAGCCCACGTGTTT
  GGGGGACACGTCAATGTCCTTAGGTGACTGGGAGGACTTGAGGCGGAAGTTCTGCATGAC
  GGTGGTGAAGAAGAGAAAGAGCNTCATTCTGCCCAGCCTTCTCGANACAGTTTCGCTTTC
  CGATGGAAAAGGCACANNAGCATCACTCTTCTTTAACTGCCCCTTCTATTCANGAAGTGC
  NTGGGATTGAAGTCTTGNGGGTTGAGAAGAAAACTGGGTCTCTCACACAAAAGCCAGCAT
  TAGGTACACTTCGGTGCCTTTAGGGAAGAAGGATCCCCGAANCTGTGGTTCTTTTGACTC
  TGCGGGCAAACTCATGGGGTTCACTTTCAATTCTTTGAATCTCGGATCCACTGCTCCGTG
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000762
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000762.4, NP_000753.2
• RefSeq Size: 1751 bp
• RefSeq ORF: 1485 bp
• Locus ID: 1548
• UniProt ID: P11509
• Cytogenetics: 19q13.2
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Caffeine metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Retinol metabolism
• Gene Summary: This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6. [provided by RefSeq, Jul 2008]