CYP46 (CYP46A1) (NM_006668) Human Untagged Clone

SKU SC120806

CYP46A1 (untagged)-Human cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CYP46
• Synonyms: CP46; CYP46
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006668, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAGCCCCGGGCTGCTGCTGCTCGGCAGCGCCGTCCTGCTCGCCTTCGGCCTCTGCTGCACCTTCGTGC; ACCGCGCTCGCAGCCGCTACGAGCACATCCCCGGGCCGCCGCGGCCCAGTTTCCTTCTAGGACACCTCCC; CTGCTTTTGGAAAAAGGATGAGGTTGGTGGCCGTGTGCTCCAAGATGTGTTTTTGGATTGGGCTAAGAAG; TATGGACCTGTTGTGCGGGTCAACGTCTTCCACAAAACCTCAGTCATCGTCACGAGTCCTGAGTCGGTTA; AGAAGTTCCTGATGTCAACCAAGTACAACAAGGACTCCAAGATGTACCGTGCGCTCCAGACTGTGTTTGG; TGAGAGACTCTTCGGCCAAGGCTTGGTGTCCGAATGCAACTATGAGCGCTGGCACAAGCAGCGGAGAGTC; ATAGACCTGGCCTTCAGCCGGAGCTCCTTGGTTAGCTTAATGGAAACATTCAACGAGAAGGCTGAGCAGC; TGGTGGAGATTCTAGAAGCCAAGGCAGATGGGCAGACCCCAGTGTCCATGCAGGACATGCTGACCTACAC; CGCCATGGACATCCTGGCCAAGGCAGCTTTTGGGATGGAGACCAGTATGCTGCTGGGTGCCCAGAAGCCT; CTGTCCCAGGCAGTGAAACTTATGTTGGAGGGAATCACTGCGTCCCGCAACACTCTGGCAAAGTTCCTGC; CAGGGAAGAGGAAGCAGCTCCGGGAGGTCCGGGAGAGCATTCGCTTCCTGCGCCAGGTGGGCAGGGACTG; GGTCCAGCGCCGCCGGGAAGCCCTGAAGAGGGGCGAGGAGGTTCCTGCCGACATCCTCACACAGATTCTG; AAAGCTGAAGAGGGAGCCCAGGACGACGAGGGTCTGCTGGACAACTTCGTCACCTTCTTCATTGCTGGTC; ACGAGACCTCTGCCAACCACTTGGCGTTCACAGTGATGGAGCTGTCTCGCCAGCCAGAGATCGTGGCAAG; GCTGCAGGCCGAGGTGGATGAGGTCATTGGTTCTAAGAGGTACCTGGATTTCGAGGACCTGGGGAGACTG; CAGTACCTGTCCCAGGTCCTCAAAGAGTCGCTGAGGCTGTACCCACCAGCATGGGGCACCTTTCGCCTGC; TGGAAGAGGAGACCTTGATTGATGGGGTCAGAGTCCCCGGCAACACCCCGCTCTTGTTCAGCACCTATGT; CATGGGGCGGATGGACACATACTTTGAGGACCCGCTGACTTTCAACCCCGATCGCTTCGGCCCTGGAGCA; CCCAAGCCACGGTTCACCTACTTCCCCTTCTCCCTGGGCCACCGCTCCTGCATCGGGCAGCAGTTTGCTC; AGATGGAGGTGAAGGTGGTCATGGCAAAGCTGCTGCAGAGGCTGGAGTTCCGGCTGGTGCCCGGGCAGCG; CTTCGGGCTGCAGGAGCAGGCCACACTCAAGCCACTGGACCCCGTGCTGTGCACCCTGCGGCCCCGCGGC; TGGCAGCCCGCACCCCCACCACCCCCCTGCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006668 unedited; GGGNCGGCACGCACAACTTCGTATAGCATACATTATACGAAGTTATGGATCAGGCCAAAT; CGGCCGAGCTCGAATTCGTCGAGAGCGGGCTGAGGCGGCGCGCGGCGCTGACAGCTGAGT; CGGCTCGCGGCCTCCCGGCCCCCTCGGCGCCCGGCCCGACCCTGGCCTGGCCTGCCCTGC; CCCGGAGCCATGAGCCCCGGGCTGCTGCTGCTCGGCAGCGCCGTCCTGCTCGCCTTCGGC; CTCTGCTGCACCTTCGTGCACCGCGCTCGCAGCCGCTACGAGCACATCCCCGGGCCGCCG; CGGCCCAGTTTCCTTCTAGGACACCTCCCCTGCTTTTGGAAAAAGGATGAGGTTGGTGGC; CGTGTGCTCCAAGATGTGTTTTTGGATTGGGCTAAGAAGTATGGACCTGTTGTGCGGGTC; AACGTCTTCCACAAAACCTCAGTCATCGTCACGAGTCCTGAGTCGGTTAAGAAGTTCCTG; ATGTCAACCAAGTACAACAAGGACTCCAAGATGTACCGTGCGCTCCAGACTGTGTTTGGT; GAGAGACTCTTCGGCCAAGGCTTGGTGTCCGAATGCAACTATGAGCGCTGGCACAAGCAG; CGGAGAGTCATAGACCTGGCCTTCAGCCGGAGCTCCTTGGTTAGCTTAATGGAAACATTC; AACGAGAAGGCTGAGCAGCTGGTGGAGATTCTAGAAGCCAAGGCAGATGGGCAGACCCCA; GTGTCCATGCANGACATGCTGACCTACACCGCCATGGACATCCTGGCCAAGGCAGCTTTT; GGGATGGAGACCAGTATGCTGCTGGGTGCCCAGAAGCTCTGTCCCAGCAGTGAAACTATG; TGGNAGGAN;
• Restriction Sites: Please inquire
• ACCN: NM_006668
• Insert Size: 3850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006668.1, NP_006659.1
• RefSeq Size: 2138 bp
• RefSeq ORF: 1503 bp
• Locus ID: 10858
• UniProt ID: Q9Y6A2
• Cytogenetics: 14q32.2
• Protein Families: Druggable Genome, P450
• Protein Pathways: Primary bile acid biosynthesis
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]