TRUB1 (NM_139169) Human Untagged Clone

SKU SC120594

TRUB1 (untagged)-Human TruB pseudouridine (psi) synthase homolog 1 (E. coli) (TRUB1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRUB1
• Synonyms: PUS4
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139169, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCGCTTCTGAGGCGGCGGTGGTGTCTTCGCCGTCTTTGAAAACAGACACATCCCCTGTCCTTGAAA; CTGCAGGAACGGTCGCAGCAATGGCTGCGACCCCGTCAGCAAGGGCTGCAGCCGCGGTGGTTGCGGCCGC; GGCCAGGACCGGATCCGAAGCCAGGGTCTCCAAGGCCGCTTTGGCTACCAAGCTGCTGTCCTTGAGCGGC; GTGTTCGCCGTGCACAAGCCCAAAGGGCCCACTTCAGCCGAGCTGCTGAATCGGTTGAAGGAGAAGCTGC; TGGCAGAAGCTGGAATGCCTTCTCCAGAATGGACCAAGAGGAAAAAGCAGACTTTGAAAATTGGGCATGG; AGGGACTCTAGACAGCGCAGCCCGAGGAGTTCTGGTTGTTGGAATTGGAAGCGGAACAAAAATGTTGACC; AGTATGTTGTCAGGGTCCAAGAGATATACTGCCATTGGAGAACTGGGGAAAGCTACTGATACACTAGATT; CTACGGGGAGGGTAACAGAAGAAAAACCTTACGATAAAATAACACAAGAAGATATTGAAGGCATTCTACA; GAAATTTACTGGAAATATAATGCAAGTGCCCCCCCTCTATTCTGCATTAAAGAAAGATGGACAAAGACTT; TCGACTTTGATGAAGAGAGGTGAAGTCGTAGAAGCAAAACCTGCCAGGCCAGTGACTGTATACAGTATCT; CCCTTCAAAAATTCCAGCCACCATTTTTCACATTAGATGTTGAATGTGGAGGAGGTTTTTATATCAGAAG; CTTGGTCAGTGACATTGGAAAAGAACTATCTTCCTGTGCCAATGTGCTAGAGCTGACCCGAACCAAACAG; GGACCATTTACGCTAGAAGAACATGCCCTTCCTGAAGACAAATGGACAATTGATGACATTGCACAGTCTC; TTGAGCATTGCTCATCTCTTTTCCCAGCAGAGTTGGCACTTAAAAAATCAAAACCTGAGTCTAATGAACA; GGTTTTGAGCTGTGAATATATAACTCTAAATGAGCCAAAGAGAGAAGATGATGTAATTAAGACGTGTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139169 unedited; GGGGGTGGTGGGGTNTANNNNNGTTATTTTTNNNNNNNGTTTGTACCCCCGCCCGTTGCC; GCATTAGGGCGGTAGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGAC; ACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGATG; AAACAGGTCTGGGCTACAAAAGTATGGCCGCTTCTGAGGCGGCGGTGGTGTCTTCGCCGT; CTTTGAAAACAGACACATCCCCTGTCCTTGAAACTGCAGGAACGGTCGCAGCAATGGCTG; CGACCCCGTCAGCAAGGGCTGCAGCCGCGGTGGTTGCGGCCGCGGCCAGGACCGGATCCG; AAGCCAGGGTCTCCAAGGCCGCTTTGGCTACCAAGCTGCTGTCCTTGAGCGGCGTGTTCG; CCGTGCACAAGCCCAAAGGGCCCACTTCAGCCGAGCTGCTGAATCGGTTGAAGGAGAAGC; TGCTGGCAGAAGCTGGAATGCCTTCTCCAGAATGGACCAAGAGGAAAAAGCAGACTTTGA; AAATTGGGCATGGAGGGACTCTAGACAGCGCAGCCCGAGGAGTTCTGGTTGTTGGAATTG; GAAGCGGAACAAAAATGTTGACCAGTATGTTGTCAGGGTCCAAGAGATATACTGCCATTG; GAGAACTGGGGAAAGCTACTGATACACTAGATTCTACGGGGAGGGTAACAGAAGAAAAAC; CTTACGATAAAATAACACAAGAAGATATTGAAGGCATTCTACAGAAATTTACTGGAAATA; TAATGCAAGTGCCCCCCCTCTATTCTGCATTAAAGAAAGATGGACANAGACTTTCGACTN; TGATGAAGAGAGTGAAGTCGTAGAAGNCAAACCTGCCAGGCCAGTGACTGTATACAGTAT; CTCCCTTCAAAAATCCAGCCACCATTTTTCACATTAGATGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_139169
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139169.3, NP_631908.1
• RefSeq Size: 3412 bp
• RefSeq ORF: 1050 bp
• Locus ID: 142940
• UniProt ID: Q8WWH5
• Cytogenetics: 10q25.3
• Domains: TruB_N
• Summary: Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).[supplied by OMIM, Mar 2008]