STARD4 (NM_139164) Human Untagged Clone

SKU SC120593

STARD4 (untagged)-Human StAR-related lipid transfer (START) domain containing 4 (STARD4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: STARD4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139164, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAAGGCCTGTCTGATGTTGCTTCTTTTGCAACTAAACTTAAAAACACTCTCATCCAGTACCATAGCA; TTGAAGAAGATAAGTGGCGAGTTGCTAAGAAAACGAAAGATGTAACTGTTTGGAGAAAACCCTCAGAAGA; ATTTAATGGATATCTCTACAAAGCCCAAGGTGTTATAGATGACCTTGTCTATAGTATAATAGACCATATA; CGCCCAGGGCCTTGTCGTTTGGATTGGGACAGCTTGATGACTTCTTTGGATATTCTGGAGAACTTTGAAG; AGAATTGCTGTGTGATGCGTTACACTACTGCTGGTCAGCTTTGGAATATAATTTCCCCAAGAGAATTTGT; TGATTTCTCCTATACTGTGGGCTATAAAGAAGGGCTTTTATCTTGTGGAATAAGTCTTGACTGGGATGAA; AAGAGACCAGAATTTGTTCGAGGATATAACCATCCCTGTGGTTGGTTTTGTGTTCCACTTAAAGACAACC; CAAACCAGAGTCTTTTGACAGGATATATTCAGACAGATCTGCGTGGGATGATTCCTCAGTCTGCGGTAGA; TACAGCCATGGCAAGCACTTTAACCAACTTCTATGGTGATTTACGAAAAGCTTTATGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139164 unedited; TAAATTTTGTATACGACTTCACTATAGGCGGCCGCGAATTCGGCACGAGGCGGTGTGAGG; TGCTTGGTAGCGCGCCGTAGCTGCTTCCACGTCCTTGCTTCACCTCAGAGAGAAGTAATG; GAAGGCCTGTCTGATGTTGCTTCTTTTGCAACTAAACTTAAAAACACTCTCATCCAGTAC; CATAGCATTGAAGAAGATAAGTGGCGAGTTGCTAAGAAAACGAAAGATGTAACTGTTTGG; AGAAAACCCTCAGAAGAATTTAATGGATATCTCTACAAAGCCCAAGGTGTTATAGATGAC; CTTGTCTATAGTATAATAGACCATATACGCCCAGGGCCTTGTCGTTTGGATTGGGACAGC; TTGATGACTTCTTTGGATATTCTGGAGAACTTTGAAGAGAATTGCTGTGTGATGCGTTAC; ACTACTGCTGGTCAGCTTTGGAATATAATTTCCCCAAGAGAATTTGTTGATTTCTCCTAT; ACTGTGGGCTATAAAGAAGGGCTTTTATCTTGTGGAATAAGTCTTGACTGGGATGAAAAG; AGACCAGAATTTGTTCGAGGATATAACCATCCCTGTGGTTGGTTTTGTGTTCCACTTAAA; GACAACCCAAACCAGAGTCTTTTGACAGGATATATTCAGACAGATCTGCGTGGGATGATT; CCTCAGTCTGCGGTAGATAAGCCATGGCAAGCACTNTAACCAACTTCTATGGTGATTTAC; GAAAAGCTNTATGAGAGGCAAAATACATTCAAACTTGTAGTACTACAGATCAACTCTCTC; AGCTACATGGNNCCTGTAAAATCATTGATTCCACTNTTCTGCATAGCCCGTAGAAAAATT; NGAATGTNNNTGGNTCACTAGTACAATGGNTTGGTTTTATTCCTAAAGGTAATAGCTATC; TAGAGAGGGCATTNTCACTNTTTTTTTTTTAAATTTTGAGAAGGCTCTTACTCTGNTGCC; ATGCTNGAGGGGCATGGGTTGATCACAGCTACTGCACCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_139164
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139164.1, NP_631903.1
• RefSeq Size: 2264 bp
• RefSeq ORF: 618 bp
• Locus ID: 134429
• UniProt ID: Q96DR4
• Cytogenetics: 5q22.1
• Summary: Cholesterol homeostasis is regulated, at least in part, by sterol regulatory element (SRE)-binding proteins (e.g., SREBP1; MIM 184756) and by liver X receptors (e.g., LXRA; MIM 602423). Upon sterol depletion, LXRs are inactive and SREBPs are cleaved, after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers, such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain, including STARD4 (Soccio et al., 2002 [PubMed 12011452]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (1) encodes the longest isoform (a). Both variants 1 and 2 encode isoform a.