RGS3 (NM_130795) Human Untagged Clone

SKU SC120479

RGS3 (untagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RGS3
• Synonyms: C2PA; RGP3
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_130795 edited; GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTATA; GAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGTGGGGGAGAG; CTGGGTTTTCATGGGGCGGCAGCCGAGGCAGGACCCGCAGCCATGAACCGCTTCAATGGG; CTCTGCAAGGTGTGCTCGGAGCGCCGCTACCGCCAGATCACCATCCCGAGGGGAAAGGAC; GGCTTTGGCTTCACCATCTGCTGCGACTCTCCAGTTCGAGTCCAGGCCGTGGATTCCGGG; GGTCCGGCGGAACGGGCAGGGCTGCAGCAGCTGGACACGGTGCTGCAGCTGAATGAGAGG; CCTGTGGAGCACTGGAAATGTGTGGAGCTGGCCCACGAGATCCGGAGCTGCCCCAGTGAG; ATCATCCTACTCGTGTGGCGCATGGTCCCCCAGGTCAAGCCAGGACCAGATGGCGGGGTC; CTGCGGCGGGCCTCCTGCAAGTCGACACATGACCTCCAGTCACCCCCCAACAAACGGGAG; AAGAACTGCACCCATGGGGTCCAGGCACGGCCTGAGCAGCGCCACAGCTGCCACCTGGTA; TGTGACAGCTCTGATGGGCTGCTGCTCGGCGGCTGGGAGCGCTACACCGAGGTGGCCAAG; CGCGGGGGCCAGCACACCCTGCCTGCACTGTCCCGTGCCACTGCCCCCACCGACCCCAAC; TACATCATCCTGGCCCCGCTGAATCCTGGGAGCCAGCTGCTCCGGCCTGTGTACCAGGAG; GATACCATCCCCGAAGAATCAGGGAGTCCCAGTAAAGGGAAGTCCTACACAGGCCTGGGG; AAGAAGTCCCGGCTGATGAAGACAGTGCAGACCATGAAGGGCCACGGGAACTACCAAAAC; TGCCCGGTTGTGAGGCCGCATGCCACGCACTCAAGCTATGGCACCTACGTCACCCTGGCC; CCCAAAGTCCTGGTGTTCCCTGTCTTTGTTCAGCCTCTAGATCTCTGTAATCCTGCCCGG; ACCCTCCTGCTGTCAGAGGAGCTGCTGCTGTATGAAGGGAGGAACAAGGCTGCCGAGGTG; ACACTGTTTGCCTATTCGGACCTGCTGCTCTTCACCAAGGAGGACGAGCCTGGCCGCTGC; GACGTCCTGAGGAACCCCCTCTACCTCCAGAGTGTGAAGCTGCAGGAAGGTTCTTCAGAA; GACCTGAAATTCTGCGTGCTCTATCTAGCAGAGAAGGCAGAGTGCTTATTCACTTTGGAA; GCGCACTCGCAGGAGCAGAAGAAGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAG; CAGCAACAGCTGGCAGCATCACCCCCGGACAGCAAGATGTTTGAGACGGAGGCAGATGAG; AAGAGGGAGATGGCCTTGGAGGAAGGGAAGGGGCCTGGTGCCGAGGATTCCCCACCCAGC; AAGGAGCCCTCTCCTGGCCAGGAGCTTCCTCCAGGACAAGACCTTCCACCCAACAAGGAC; TCCCCTTCTGGGCAGGAACCCGCTCCCAGCCAAGAACCACTGTCCAGCAAAGACTCAGCT; ACCTCTGAAGGATCCCCTCCAGGCCCAGATGCTCCGCCCAGCAAGGATGTGCCACCATGC; CAGGAACCCCCTCCAGCCCAAGACCTCTCACCCTGCCAGGACCTACCTGCTGGTCAAGAA; CCCCTGCCTCACCAGGACCCTCTACTCACCAAAGACCTCCCTGCCATCCAGGAATCCCCC; ACCCGGGACCTTCCACCCTGTCAAGATCTGCCTCCTAGCCAGGTCTCCCTGCCAGCCAAG; GCCCTTACTGAGGACACCATGAGCTCCGGGGACCTACTAGCAGCTACTGGGGACCCACCT; GCGGCCCCCAGGCCAGCCTTCGTGATCCCTGAGGTCCGGCTGGATAGCACCTACAGCCAG; AAGGCAGGGGCAGAGCAGGGCTGCTCGGGAGATGAGGAGGATGCAGAAGAGGCCGAGGAG; GTGGAGGAGGGGGAGGAAGGGGAGGAGGACGAGGATGAGGACACCAGCGATGACAACTAC; GGAGAGCGCAGTGAGGCCAAGCGCAGCAGCATGATCGAGACGGGCCAGGGGGCTGAGGGT; GGCCTCTCACTGCGTGTGCAGAACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTG; CTGCAGGAGCCCCGAGGGCCCTGCTTTGCCTCCGACACCACCTTGCACTGCTCAGACGGT; GAGGGCGCCGCCTCCACCTGGGGCATGCCTTCGCCCAGCACCCTCAAGAAAGAGCTGGGC; CGCAATGGTGGCTCCATGCACCACCTTTCCCTCTTCTTCACAGGACACAGGAAGATGAGC; GGGGCTGACACCGTTGGGGATGATGACGAAGCCTCCCGGAAGAGAAAGAGCAAAAACCTA; GCCAAGGACATGAAGAACAAGCTGGGGATCTTCAGACGGCGGAATGAGTCCCCTGGAGCC; CCTCCCGCGGGCAAGGCAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGCC; CTCAAGTGGGGCGAGTCCTTGGAGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTC; CAAGCCTTCCTTCGCACTGAGTTCAGTGAGGAGAATCTGGAGTTCTGGTTGGCTTGTGAG; GACTTCAAGAAGGTCAAGTCACAGTCCAAGATGGCATCCAAGGCCAAGAAGATCTTTGCT; GAATACATCGCGATCCAGGCATGCAAGGAGGTCAACCTGGACTCCTACACGCGGGAGCAC; ACCAAGGACAACCTGCAGAGCGTCACGCGGGGCTGCTTCGACCTGGCACAGAAGCGCATC; TTCGGGCTCATGGAAAAGGACTCGTACCCTCGCTTTCTCCGTTCTGACCTCTACCTGGAC; CTTATTAACCAGAAGAAGATGAGTCCCCCGCTTTAGGGGCCACTGGAGTCGAGCTCAGCG; TTCACACCAGGCGGGCTGGGTCCCCTGCCCACCTGCCTCCCTGCCCCCTGTGACGGAGGG; GGCAAGCAAGCCCCCAGAGGCCGTGTCTCTGGACAGACGGATAGACATACGGAAGCGAGG; CCTGGACCAAGAGAGGCCCAGGCTACTGGAGGAGTAGAAGGATGGGCCCCGTGGGGTCCC; CACTGCCCCGGTACGAGGGGGCCCAAGACCCTGGCAGGTCA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130795 unedited; CCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA; GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT; TCGGCACGAGGTGGGGGAGAGCTGGGTTTTCATGGGGCGGCAGCCGAGGCAGGACCCGCA; GCCATGAACCGCTTCAATGGGCTCTGCAAGGTGTGCTCGGAGCGCCGCTACCGCCAGATC; ACCATCCCGAGGGGAAAGGACGGCTTTGGCTTCACCATCTGCTGCGACTCTCCAGTTCGA; GTCCAGGCCGTGGATTCCGGGGGTCCGGCGGAACGGGCAGGGCTGCAGCAGCTGGACACG; GTGCTGCAGCTGAATGAGAGGCCTGTGGAGCACTGGAAATGTGTGGAGCTGGCCCACGAG; ATCCGGAGCTGCCCCAGTGAGATCATCCTACTCGTGTGGCGCATGGTCCCCCAGGTCAAG; CCAGGACCAGATGGCGGGGTCCTGCGGCGGGCCTCCTGCCAGTCGACACATGACCTCCAG; TCACCCCCCACAAACGGGAGAAGAACTGCACCCATGCTGTCCAGGCACGGNCTGACCAGC; CCCCACCTGCCCCCGGTATGTCACCCCTCTCGAGGGCTGTCCTCCGCTTCCTCCCCCTAT; CACCCCGGTCCTCCCCTCCCCCCCCCCCCCCCCTGCCCGCCCCCCCCCCCCATTCCCCCT; CTCCCCCCATTTCCTCCCTCACCCCTCAACCCCCCCCCCCCTGTCCCTTTTCCCCCCACC; CGCACCACCCCCCCCCCTCGCGCCCCCCAAAGCCCCCATCCCAAGCCTCCCCCCCCCCCG; TCTCCCCCGACCCACCCCCCACCTCCTCTCCCCCCCAACCCCCCCCCGCCCCCCCCCTAC; CCTATTCCTCCTCCCCCCTTCCCCCCCCTCCCCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130795 unedited; GATCGTAGGCTTAAAAANCNNCCTCGACTTGGNCTACTGAGTGCGCNCTGAGGGTGCCAT; GCTTTCCCAAAGCCCCCGAGGGACACTCCACCCGGGGGTGCCAGATCTCTGTGTGTAGCC; AGACTGCAGTGTCCCCCCAGGCTGCCTTATTCCAGAAGGCCAGCTGCCTGCCACCGGTGC; CCGGGACGAGCATATCCCCTGAAGCTGGAGAATGGTTTTTTCTTTGCATGGCAGCTTCTC; CCAAGGCATTGGCTAAAATATTGCACTTGTAAATCATGAAAACTGATGAGGCAAGGGTTT; CCTATTACAGGACGACGTGCCAAGCATCCAACTACTATTTAAATAAATAAAACTCAAAGT; GGGGGTCTGAGCTGCAAGCTCCAATGGCCCTCTAGGAGGGCCCGAGAGCCCAGGCCTGGA; CCTCCTGCCCCATCAGCCAGTTCTTTAAGGAACTTGGTCAACGCGAAGCCTCCGCGGTCT; CCGCAGCAGGGAGCAGCAGCTCCAGATCTGGCTTGGCCAGGGCCCCTGACCTGCCAGGGT; CTTGGGCCCCCTCGTTCCGGGGCAGTGGGGACCCCACGGGGCCCATCCTTCTACTCCTCC; AGTAGCCTGGGCCTCTCTTGGTCCATGCCTCGCTTCCGTCTGTCTGTCCGTCTGTCCCGA; GACTCGGCCTCTGGGGGCTTGCTTGCCCCCTCCGTCCAGGGGGCAGGGAGGCATGGTGGG; CAGGGACCCATTCCGTCTGGGGTGAACGCTTGACCTGGGTTCCTTTGGGCCCCTTAGGCG; GGGGGACTCCATCTTTCTTCTGGTTATTAACGGTCCGCGTTGGAGGTTCGGACCGGCGCA; AATCGTTGGGTCCCGGTCCCTCTTTTTTTGTTTCCTTTAAAATTCTCCTTTTGGCGCCAG; TGTTAAACACTCCCCAGTATGTAATACCATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_130795
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_130795.2, NP_570613.2
• RefSeq Size: 3741 bp
• RefSeq ORF: 2754 bp
• Locus ID: 5998
• UniProt ID: P49796
• Cytogenetics: 9q32
• Domains: PDZ, RGS
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Summary: This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]; Transcript Variant: This variant (1) lacks several 5' exons but includes an alternate 5' exon, and it thus differs in the 5' UTR and 5' coding region, compared to variant 6. The encoded isoform (1, also known as PDZ-RGS3) has a shorter and distinct N-terminus, compared to isoform 6.