FENS1 (WDFY1) (NM_020830) Human Untagged Clone

SKU SC120411

WDFY1 (untagged)-Human WD repeat and FYVE domain containing 1 (WDFY1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FENS1
• Synonyms: FENS-1; FENS1; WDF1; ZFYVE17
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020830, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCCGAAATCCACTCCAGGCCGCAGAGCAGCCGCCCGGTGCTGCTGAGCAAGATCGAGGGGCACC; AGGACGCCGTCACGGCCGCGCTGCTCATCCCCAAGGAGGACGGCGTGATCACGGCCAGCGAGGACAGAAC; CATCCGGGTATGGCTGAAAAGAGACAGTGGTCAATACTGGCCCAGCATTTACCACACAATGGCCTCTCCT; TGCTCTGCTATGGCTTACCATCATGACAGCAGACGGATATTTGTGGGCCAGGATAATGGAGCTGTAATGG; AATTTCACGTTTCTGAAGATTTTAATAAAATGAACTTTATCAAGACCTACCCAGCTCATCAGAACCGGGT; GTCTGCGATTATCTTCAGCTTGGCCACAGAGTGGGTGATCAGTACCGGCCACGACAAGTGTGTGAGCTGG; ATGTGCACGCGGAGCGGGAACATGCTCGGGAGGCACTTCTTCACGTCCTGGGCTTCGTGTCTGCAATATG; ACTTTGACACTCAGTATGCTTTCGTTGGTGATTATTCTGGGCAGATCACCCTGCTGAAGCTTGAACAGAA; CACGTGTTCAGTCATCACAACCCTCAAAGGACATGAAGGTAGTGTCGCCTGCCTCTGGTGGGACCCTATT; CAGCGGTTACTCTTCTCAGGAGCATCTGACAACAGCATCATCATGTGGGACATCGGAGGAAGGAAAGGCC; GGACGCTGTTACTTCAGGGCCATCATGACAAGGTGCAGTCGCTGTGCTACCTTCAGCTCACCAGGCAGCT; CGTCTCCTGTTCCTCGGACGGCGGAATTGCAGTGTGGAACATGGATGTTAGCAGAGAAGAGGCTCCTCAG; TGGTTGGAAAGTGATTCTTGTCAGAAATGTGAGCAGCCATTTTTCTGGAACATAAAGCAGATGTGGGACA; CCAAGACGCTGGGGCTAAGACAACATCACTGCAGGAAATGCGGGCAGGCTGTCTGCGGGAAGTGCAGCAG; CAAGCGCTCAAGTTACCCAGTCATGGGCTTCGAGTTCCAAGTCCGGGTTTGTGATTCTTGTTACGACTCC; ATCAAAGATGAAGATCGGACTTCTCTAGCGACCTTTCATGAAGGAAAACATAACATTTCCCACATGTCCA; TGGACATTGCCAGGGGACTGATGGTGACCTGTGGGACCGACCGCATTGTAAAGATCTGGGACATGACACC; TGTGGTGGGCTGCAGTCTGGCGACTGGGTTTTCTCCGCACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020830 unedited; TCAGCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGCGAACATGG; CGGCCGAAATCCACTCCAGGCCGCAGAGCAGCCGCCCGGTGCTGCTGAGCAAGATCGAGG; GGCACCAGGACGCCGTCACGGCCGCGCTGCTCATCCCCAAGGAGGACGGCGTGATCACGG; CCAGCGAGGACAGAACCATCCGGGTATGGCTGAAAAGAGACAGTGGTCAATACTGGCCCA; GCATTTACCACACAATGGCCTCTCCTTGCTCTGCTATGGCTTACCATCATGACAGCAGAC; GGATATTTGTGGGCCAGGATAATGGAGCTGTAATGGAATTTCACGTTTCTGAAGATTTTA; ATAAAATGAACTTTATCAAGACCTACCCAGCTCATCAGAACCGGGTGTCTGCGATTATCT; TCAGCTTGGCCACAGAGTGGGTGATCAGTACCGGCCACGACAAGTGTGTGAGCTGGATGT; GCACACGGAGCGGGAACATGCTCGGGAGGCACTTCTTCACGTCCTGGGCTTCGTGTCTGC; AATATGACTTTGACACTCAGTATGCTTTCGTTGGTGATTATTCTGGGCAGATCACCCTGC; TGAAGCTTGAACAGAACACGTGTTCAGTCATCACAACCCTCAAAGGACATGAAGGTAGTG; TCGCCTGCCTCTGGTGGGACCCTATTCAGCGGTTACTCTTCTCAGGAGCATCTGACAACA; GCATCATCATGTGGGACATCGGAGGAAGGAAAGGCCGNACGCTGTTACTTCAGGGCCATC; ATGACAAGGTGCAGTCGCTGTGCTACCTTCAGCTCACCAGGCAGCTCGTCTCCTGTTCCT; CGNACGGCGGNATNGCAGTGTGGAACATGGATGTTAGCANAGAAGAGCTNCTCAGTGTTT; GGAAAGGATTCTGTCAAAATGGAGCAGCCATTTTCTGGACATAAGCAGAGTGGGACACCA; GACGCTGGGCTAGAACACTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020830 unedited; CCCCACACAACCNCTCCCCACACCCCCCCCCCCCCCCTTTGACTTGGNACCGCGGCCGCA; TNCTACGATCGAGTTTTTTTTTTTTTTTTTTTAACAAGCAAGGAAGTTTTATTGTGTACA; TTTTCTCAAAAAAAAAAAAACCCACAAAAACAGAACCCACAAATAGGAGCAAAGAACAGT; TTTCTAGCATCTCTGGGATACACAGGGCACCATATCTTATAGAAACATAGCTAGTACAAG; AACAAAGTGTACTAATTAACATTACCCCTTGCTTCCCCAGTCAAAAAAGGCTATAGAGAA; AAACACTTTAAATTGTACTCTAATACAAATTTGTTGCACAATTAAACTTCAACTTACTCA; AAGAGTTATTGTATTGTAAACTGCAGAAAATAGAATAGGCATTCAATAAATCTGCTCACA; ATTAACAATTAAGCAATAGACACTTGAATAAATATAGCATGTATTACTTATTTGTTAAGC; CTTACACTTACGATGAGCAGATGGTTTATCACTATATAGTAACAGATTTTTTTTACACTT; TAGGAGATACCATATCCAATAACGGAAGTAGAAGTACTTTAACGAAAACTGATTTTAGAA; AAATATCTCCTTGGGTCTGAGCTACTATGCTATTTATTACATATTTGGGTCAGCTTCACC; AGTTTAAAAATATAGGTACTAACAAACTGAATTCTTTCATGGGAGAACAAAGTAATACAT; TCTACAGCCATAGTATTTCTTTATATCCTTTAGGCACCAGGACCGAACTTGGACATAGTA; AAGAGAATTACGACGCAACGAAAAATTTGGTTCTACCTAATGCTATAGGGGTATTGCTAT; CATTTGAACTAAAAAAGAAACATTTTAAAACCTTGTTTATAATGAAAAAACCGGAATTCC; CTTACCCCATATTATCCTACAAGGCAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_020830
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020830.3, NP_065881.1
• RefSeq Size: 4607 bp
• RefSeq ORF: 1233 bp
• Locus ID: 57590
• UniProt ID: Q8IWB7
• Cytogenetics: 2q36.1
• Domains: FYVE, WD40
• Summary: The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain. [provided by RefSeq, Jan 2015]