NOSTRIN (NM_052946) Human Untagged Clone

SKU SC120170

NOSTRIN (untagged)-Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NOSTRIN
• Synonyms: DaIP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120170 sequence for NM_052946 edited (data generated by NextGen Sequencing); ATGAAATCCACAGCGGACCTGCATCAAAAACTTGGCAAAGCAATTGAATTGGAAGCAATA; AAACCGACTTATCAAGTCCTAAATGTACAAGAGAAGAAGAGAAAATCACTTGACAATGAA; GTTGAAAAGACAGCAAATCTTGTCATTAGCAACTGGAATCAGCAAATTAAGGCCAAGAAG; AAATTAATGGTTAGTACCAAGAAACATGAAGCACTTTTCCAGCTTGTAGAAAGCTCCAAG; CAATCTATGACTGAGAAGGAGAAGCGGAAGCTCCTCAATAAACTGACAAAATCAACTGAA; AAGTTGGAAAAGGAAGATGAAAATTACTACCAAAAAAACATGGCGGGTTATTCTACCAGA; CTGAAATGGGAAAACACACTAGAGAACTGCTACCAGAGCATTCTGGAGCTGGAGAAGGAA; AGAATTCAACTTTTATGCAATAACTTAAACCAGTACAGCCAACATATTTCTCTTTTTGGC; CAAACCCTGACCACATGCCACACGCAGATTCACTGTGCCATCAGCAAGATTGACATTGAA; AAAGATATCCAGGCTGTAATGGAAGAAACTGCAATTTTATCTACAGAAAACAAATCTGAG; TTCCTGTTAACGGATTACTTTGAAGAAGATCCTAACAGTGCAATGGATAAAGAGAGACGA; AAGTCTTTACTAAAACCAAAATTATTGAGACTGCAGAGAGACATTGAAAAAGCCTCAAAA; GACAAGGAAGGCCTGGAACGAATGCTTAAAACGTACTCCAGCACCTCCTCCTTCTCTGAT; GCAAAGAGCCAGAAAGACACAGCAGCGTTAATGGATGAGAACAATTTGAAACTAGACCTT; TTGGAAGCGAACTCCTACAAACTGTCATCAATGTTAGCAGAACTTGAGCAAAGACCTCAA; CCCAGCCATCCTTGTAGTAATTCCATCTTCAGGTGGAGGGAAAAGGAGCATACTCATAGC; TATGTGAAAATATCTCGGCCTTTTTTAATGAAGAGATTAGAGAATATTGTGAGCAAGGCA; TCTTCTGGTGGGCAGAGCAATCCAGGTTCTTCAACTCCAGCCCCTGGTGCAGCCCAGCTC; AGCAGCAGACTTTGCAAGGCCTTGTATTCTTTTCAAGCCAGGCAAGATGATGAGTTGAAT; TTGGAAAAGGGTGACATTGTGATTATACACGAGAAAAAAGAAGAAGGATGGTGGTTTGGA; TCTTTGAATGGGAAAAAAGGCCATTTTCCTGCCGCTTATGTGGAGGAGTTACCTTCAAAT; GCTGGCAACACAGCTACAAAGGCATAA; ; Clone variation with respect to NM_052946.3; 1184 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052946 unedited; NNNTTGTTCACATTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACCAGGTATAC; AAGAACCTAAAGAGTTTTCTCAAAATGGAGAGAATTTCTGCAAACAGGTCACATCTGTTC; TTCAGCAAAGGGCAAACCTGGAAATTAGCTATGCCAAAGGACTTCAGAAACTGGCAAGCA; AGCTGAGCAAAGCATTACAGAACACGAGAAAAAGTTGTGTTAGCAGTGCCTGGGCCTGGG; CCTCAGAGGGAATGAAATCCACAGCGGACCTGCATCAAAAACTTGGCAAAGCAATTGAAT; TGGAAGCAATAAAACCGACTTATCAAGTCCTAAATGTACAAGAGAAGAAGAGAAAATCAC; TTGACAATGAAGTTGAAAAGACAGCAAATCTTGTCATTAGCAACTGGAATCAGCAAATTA; AGGCCAAGAAGAAATTAATGGTTAGTACCAAGAAACATGAAGCACTTTTCCAGCTTGTAG; AAAGCTCCAAGCAATCTATGACTGAGAAGGAGAAGCGGAAGCTCCTCAATAAACTGACAA; AATCAACTGAAAAGTTGGAAAAGGAAGATGAAAATTACTACCAAAAAAACATGGCGGGTT; ATTCTACCAGACTGAAATGGGAAAACACACTAGAGAACTGCTACCAGAGCATTCTGGAGC; TGGAGAAGGAAAGAATTCAACTTTTATGCAATAACTTANACCAGTACAGCCAACATATTT; CTCTTTTTGGCCAAACCCTGACCACATGCCACACGCAGATTCACTGTGCCATCAGCAAGA; TTGACATTGAAAAAGAATCCNAGCTGTAATGGAAGAACTGNCATTTATCTACAGAAAACA; ATCTGAGTTCCTGTTAAACGGATACTTTGAAGAAGATCCCTACAGTGCATGGGATAAAGA; AGACGAAAAGTCTTACTAAACAAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_052946 unedited; TANGATCAGTTTTATTTTTTTTTTTTTTAAAGGTAAGAGCACTTTATTCTCTATTTGAAC; CACACTGTATTGTTGATTACCGAGTGTGAAGGTAGTATGTTCAGAGTCTTGTTTTATGCC; TTTGTAGCTGTGTTGCCAGCATTTGAAGGTAACTCCTCCACATAAGCGGCAGGAAAATGG; CCTTTTTTCCCATTCAAAGATCCAAACCACCATCCTTCTTCTTTTTTCTCGTGTATAATC; ACAATGTCACCCTTTTCCAAATTCAACTCATCATCTTGCCTGGCTTGAAAAGAATACAAG; GCCTTGCAAAGTCTGCTGCTGAGCTGGGCTGCACCAGGGGCTGGAGTTGAAGAACCTGGA; TTGCTCTGCCCACCAGAAGATGCCTTGCTCACAATATTCTCTAATCTCTTCATTAAAAAA; GGCCGAGATATTTTCACATAGCTATGAGTATGCTCCTTTTCCCTCCACCTGAAGATGGAA; TTACTACAAGGATGGCTGGGTTGAGGTCTTTGCTCAAGTTCTGCTAACATTGATGACAGT; TTGTAGGAGTTCGCTTCCAAAAGGTCTAGTTTCAAATTGTTCTCATCCATTAACGCTGCT; GTGTCTTTCTGGCTCTTTGCATCAAAGAAGGAGGAGGTGCTGGAGTACGTTTTAAGCATT; CGTTCCAGGCCTTCCTTGTCTTTTGAGGCTTTTTCAATGTCTCTCTGCAGTCTCAATAAT; TTTGGTTTTAGTAAAGACTTTCGTCTCTCTTTACCATTGCACTGGTTAGATCTTCTTCAA; AGTAATCCCGTAACAGGGACTCAGATTTGTTTTCTGTAGATAAAATTGCAGTTTCTTCCA; TAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_052946
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_052946.2, NP_443178.1
• RefSeq Size: 1588 bp
• RefSeq ORF: 1287 bp
• Locus ID: 115677
• UniProt ID: Q8IVI9
• Cytogenetics: 2q24.3
• Domains: SH3
• Summary: Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission, inflammatory response, and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production, endothelial NO synthase (ENOS; MIM 163729), and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures, thereby attenuating ENOS-dependent NO production.[supplied by OMIM, Apr 2004]; Transcript Variant: This variant (1) lacks an internal exon in the 5' region, resulting in a downstream AUG start codon, as compared to isoform 2. The resulting isoform (1) has a shorter N-terminus, as compared to isoform 2.