IGSF8 (NM_052868) Human Untagged Clone

SKU SC120151

IGSF8 (untagged)-Human immunoglobulin superfamily, member 8 (IGSF8), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: IGSF8
• Synonyms: CD81P3; CD316; EWI-2; EWI2; KCT-4; LIR-D1; PGRL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120151 sequence for NM_052868 edited (data generated by NextGen Sequencing); ATGGGCGCCCTCAGGCCCACGCTGCTGCCGCCTTCGCTGCCGCTGCTGCTGCTGCTAATG; CTAGGAATGGGATGCTGGGCCCGGGAGGTGCTGGTCCCCGAGGGGCCCTTGTACCGCGTG; GCTGGCACAGCTGTCTCCATCTCCTGCAATGTGACCGGCTATGAGGGCCCTGCCCAGCAG; AACTTCGAGTGGTTCCTGTATAGGCCCGAGGCCCCAGATACTGCACTGGGCATTGTCAGT; ACCAAGGATACCCAGTTCTCCTATGCTGTCTTCAAGTCCCGAGTGGTGGCGGGTGAGGTG; CAGGTGCAGCGCCTACAAGGTGATGCCGTGGTGCTCAAGATTGCCCGCCTGCAGGCCCAG; GATGCCGGCATTTATGAGTGCCACACCCCCTCCACTGATACCCGCTACCTGGGCAGCTAC; AGCGGCAAGGTGGAGCTGAGAGTTCTTCCAGATGTCCTCCAGGTGTCTGCTGCCCCCCCA; GGGCCCCGAGGCCGCCAGGCCCCAACCTCACCCCCACGCATGACGGTGCATGAGGGGCAG; GAGCTGGCACTGGGCTGCCTGGCGAGGACAAGCACACAGAAGCACACACACCTGGCAGTG; TCCTTTGGGCGATCTGTGCCCGAGGCACCAGTTGGGCGGTCAACTCTGCAGGAAGTGGTG; GGAATCCGGTCAGACTTGGCCGTGGAGGCTGGAGCTCCCTATGCTGAGCGATTGGCTGCA; GGGGAGCTTCGTCTGGGCAAGGAAGGGACCGATCGGTACCGCATGGTAGTAGGGGGTGCC; CAGGCAGGGGACGCAGGCACCTACCACTGCACTGCCGCTGAGTGGATTCAGGATCCTGAT; GGCAGCTGGGCCCAGATTGCAGAGAAAAGGGCCGTCCTGGCCCACGTGGATGTGCAGACG; CTGTCCAGCCAGCTGGCAGTGACAGTGGGGCCTGGTGAACGTCGGATCGGCCCAGGGGAG; CCCTTGGAACTGCTGTGCAATGTGTCAGGGGCACTTCCCCCAGCAGGCCGTCATGCTGCA; TACTCTGTAGGTTGGGAGATGGCACCTGCGGGGGCACCTGGGCCCGGCCGCCTGGTAGCC; CAGCTGGACACAGAGGGTGTGGGCAGCCTGGGCCCTGGCTATGAGGGCCGACACATTGCC; ATGGAGAAGGTGGCATCCAGAACATACCGGCTACGGCTAGAGGCTGCCAGGCCTGGTGAT; GCGGGCACCTACCGCTGCCTCGCCAAAGCCTATGTTCGAGGGTCTGGGACCCGGCTTCGT; GAAGCAGCCAGTGCCCGTTCCCGGCCTCTCCCTGTACATGTGCGGGAGGAAGGTGTGGTG; CTGGAGGCTGTGGCATGGCTAGCAGGAGGCACAGTGTACCGCGGGGAGACTGCCTCCCTG; CTGTGCAACATCTCTGTGCGGGGTGGCCCCCCAGGACTGCGGCTGGCCGCCAGCTGGTGG; GTGGAGCGACCAGAGGACGGAGAGCTCAGCTCTGTCCCTGCCCAGCTGGTGGGTGGCGTA; GGCCAGGATGGTGTGGCAGAGCTGGGAGTCCGGCCTGGAGGAGGCCCTGTCAGCGTAGAG; CTGGTGGGGCCCCGAAGCCATCGGCTGAGACTACACAGCTTGGGGCCCGAGGATGAAGGC; GTGTACCACTGTGCCCCCAGCGCCTGGGTGCAGCATGCCGACTACAGCTGGTACCAGGCG; GGCAGTGCCCGCTCAGGGCCTGTTACAGTCTACCCCTACATGCATGCCCTGGACACCCTA; TTTGTGCCTCTGCTGGTGGGTACAGGGGTGGCCCTAGTCACTGGTGCCACTGTCCTTGGT; ACCATCACTTGCTGCTTCATGAAGAGGCTTCGAAAACGGTGA; ; Clone variation with respect to NM_052868.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052868 unedited; GGGGTTTCACATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGC; TGCCCCGCTTCCTGCCCTCAACCTGGGCATGCGCCCCCCACCCTTCCGGCCCCCCAGAAC; CCGCGCCATCCCCCGGAGCCTCCCCAGAGCTGGCCGCGCAGGATGGGCGCCCTCAGGCCC; ACGCTGCTGCCGCCTTCGCTGCCGCTGCTGCTGCTGCTAATGCTAGGAATGGGATGCTGG; GCCCGGGAGGTGCTGGTCCCCGAGGGGCCCTTGTACCGCGTGGCTGGCACAGCTGTCTCC; ATCTCCTGCAATGTGACCGGCTATGAGGGCCCTGCCCAGCAGAACTTCGAGTGGTTCCTG; TATAGGCCCGAGGCCCCAGATACTGCACTGGGCATTGTCAGTACCAAGGATACCCAGTTC; TCCTATGCTGTCTTCAAGTCCCGAGTGGTGGCGGGTGAGGTGCAGGTGCAGCGCCTACAA; GGTGATGCCGTGGTGCTCAAGATTGCCCGCCTGCAGGCCCAGGATGCCGGCATTTATGAG; TGCCACACCCCCTCCACTGATACCCGCTACCTGNGCAGCTACAGCGGCAAGGTGGAGCTG; AGAGTTCTTCCAGATGTCCTCCAGGTGTCTGCTGCCCCCCCAGGGCCCCGAGGCCGCCAG; GCCCCAACCTCACCCCCACGCATGACGGTGCATGAGGGGCAGGAGCTGGCACTGGGCTGC; CTGGCGAGGACAAGCACACAGAAGCACACACACCTGGCAGTGTCCTTTGGGCGATCTGTG; CCCCGAGGCACCAGTTGGGCGGTCAACTCTGCAGGAAGTGGTGGGAATCCGGTCAGACTT; GGCCGTGGNAGCTGGAGCTCCCTATGCTNGAGCGATGGCTGCAGGGGAGCTTCGTCTGGG; CAAGGAAGGGACCGATCGGTACCGCATGGTANTAGGGGGGTGCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_052868 unedited; AAACTACGTATGACCGCGGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTTATAAA; ACAAGTTTATTCACATTTTAGAAAAACTAATTCCAGGACAGGAAATGGCCTCCCTATAGG; ATCCCTAAGAGATCAAGAACAGAAGGCCAGAGGGAGGGGCTTGGGAGGGAAGGAGTGGGG; AAGGGGAGGCACGTCTCCCATTCTGGGTAGTGGGAGGTCAAATAAATTAAAGGAAGAGTG; GACAGAGGGAGAGGGTGTCCAGGCAACCAGAGGAGGGCTTGGAGCTGGGCCGGAAGACAG; TCGACACCTGCAAGACCTGGGGAGTAAGGGATCACCGTTTTCGAAGCCTCTTCATGAAGC; AGCAAGTGATGGTACCAAGGACAGTGGCACCAGTGACTAGGGCCACCCCTGTACCCACCA; GCAGAGGCACAAATAGGGTGTCCAGGGCATGCATGTAGGGGTAGACTGTAACAGGCCCTG; AGCGGGCACTGCCCGCCTGGTACCAGCTGTAGTCGGCATGCTGCACCCAGGCGCTGGGGG; CACAGTGGTACACGCCTTCATCCTCGGGCCCCAAGCTGTGTAGTCTCAGCCGATGGCTTC; GGGGCCCCACCAGCTCTACGCTGACAGGGCCTCCTCCAGGCCGGACTCCCAGCTCTGCCA; CACCATCCTGGCCTACGCCACCCACCAGCTGGGCAGGGACAGAGCTGAGCTCTCCGTCCT; CTGGTCGCTCCACCCACAGCTGGCGGCAGCCGCAGTCCTGGGGGGCCACCCGCCAGAGAT; GTTGCACAGCAGGGAGGCAGTCTCCCCGCGTACACTGTGCCTCCTGCTAGCCAGCCACAG; CCTTCAGCACACACCTTCTTCCGCACATGTCAGGGAAAGCCCGGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_052868
• Insert Size: 2350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_052868.1, NP_443100.1
• RefSeq Size: 2297 bp
• RefSeq ORF: 1842 bp
• Locus ID: 93185
• UniProt ID: Q969P0
• Cytogenetics: 1q23.2
• Domains: ig, IG
• Protein Families: Transmembrane
• Summary: This gene encodes a member the EWI subfamily of the immunoglobulin protein superfamily. Members of this family contain a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a variable number of immunoglobulin domains. This protein interacts with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. The encoded protein may also function as a tumor suppressor by inhibiting the proliferation of certain cancers. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.