Hemoglobin subunit gamma 2 (HBG2) (NM_000184) Human Untagged Clone

SKU SC120038

HBG2 (untagged)-Human hemoglobin, gamma G (HBG2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Hemoglobin subunit gamma 2
• Synonyms: HBG-T1; TNCY
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120038 sequence for NM_000184 edited (data generated by NextGen Sequencing); ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT; GTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAG; AGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAA; GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGAT; GATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGAT; CCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGC; AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTGGCCAGT; GCCCTGTCCTCCAGATACCACTGA; ; Clone variation with respect to NM_000184.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000184 unedited; GAAGCCTGGGTTCTTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGG; TGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAAAGGCTCCTGGTTGTCTACCCATGG; ACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAAC; CCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCAC; CTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCAT; GTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCAT; TTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTG; GCCAGTGCCCTGTCCTCCAGATACCACTGAGCTCACTGCCCATGATGCAGAGCTTTCAAG; GATAGGCTTTATTCTGCAAGCAATCAAATAATAAATCTATTCTGCTAAGAGATCACAAAA; AAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACA; GATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCC; ACTCCAGTGCCCACCAGCCTTGTCCTATAAAATTAAGTTGCATCATTTTGTCTGACTAGG; TGTCCTTCTTATATATGGGTGAGGGGGGGNGNGGNGNNTNNNNANNN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000184 unedited; NTTAGCTCTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTGGG; ATCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAA; GCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACTCCA; GTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATGGATT; GCCAAAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGC; TTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTT; ATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCC; ATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGG; TAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCC; CACAGGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATGGCGTCTGGACTA; GGAGCTTATTGATAACCTCAGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTA; TTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACAC; GCCTACCGNCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTNCCGTT; GATTTTGGTGCCAAACAAACTTCCATTGACGTCATGGGGGTGGGAGACTTGGAAATCCCC; GTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGGCCAAACCGCATCACATGGTATT; AGCGAGACTAATCCCTANATGTACTGCAGTNAGGAAGTCCCGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000184
• Insert Size: 620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000184.2, NP_000175.1
• RefSeq Size: 583 bp
• RefSeq ORF: 444 bp
• Locus ID: 3048
• UniProt ID: P69892
• Cytogenetics: 11p15.4
• Domains: globin
• Summary: The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]