MSH6 (NM_000179) Human Untagged Clone

CAT#: SC120035

MSH6 (untagged)-Human mutS homolog 6 (E. coli) (MSH6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSH6
• Synonyms: GTBP; GTMBP; HNPCC5; HSAP; MMRCS3; p160
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120035 sequence for NM_000179 edited (data generated by NextGen Sequencing)
  ATGTCGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCCGGCGCTGAGTGATGCC
  AACAAGGCCTCGGCCAGGGCCTCACGCGAAGGCGGCCGTGCCGCCGCTGCCCCCGGGGCC
  TCTCCTTCCCCAGGCGGGGATGCGGCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTG
  GCGCGCTCCGCGTCACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTA
  GCGCCTGCTGCCCCCACCAGTTGTGACTTCTCACCAGGAGATTTGGTTTGGGCCAAGATG
  GAGGGTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTTTGATGGAACATTCATC
  CGCGAGAAAGGGAAATCAGTCCGTGTTCATGTACAGTTTTTTGATGACAGCCCAACAAGG
  GGCTGGGTTAGCAAAAGGCTTTTAAAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAG
  AAGGGAGGTCATTTTTACAGTGCAAAGCCTGAAATACTGAGAGCAATGCAACGTGCAGAT
  GAAGCCTTAAATAAAGACAAGATTAAGAGGCTTGAATTGGCAGTTTGTGATGAGCCCTCA
  GAGCCAGAAGAGGAAGAAGAGATGGAGGTAGGCACAACTTACGTAACAGATAAGAGTGAA
  GAAGATAATGAAATTGAGAGTGAAGAGGAAGTACAGCCTAAGACACAAGGATCTAGGCGA
  AGTAGCCGCCAAATAAAAAAACGAAGGGTCATATCAGATTCTGAGAGTGACATTGGTGGC
  TCTGATGTGGAATTTAAGCCAGACACTAAGGAGGAAGGAAGCAGTGATGAAATAAGCAGT
  GGAGTGGGGGATAGTGAGAGTGAAGGCCTGAACAGCCCTGTCAAAGTTGCTCGAAAGCGG
  AAGAGAATGGTGACTGGAAATGGCTCTCTTAAAAGGAAAAGCTCTAGGAAGGAAACGCCC
  TCAGCCACCAAACAAGCAACTAGCATTTCATCAGAAACCAAGAATACTTTGAGAGCTTTC
  TCTGCCCCTCAAAATTCTGAATCCCAAGCCCACGTTAGTGGAGGTGGTGATGACAGTAGT
  CGCCCTACTGTTTGGTATCATGAAACTTTAGAATGGCTTAAGGAGGAAAAGAGAAGAGAT
  GAGCACAGGAGGAGGCCTGATCACCCCGATTTTGATGCATCTACACTCTATGTGCCTGAG
  GATTTCCTCAATTCTTGTACTCCTGGGATGAGGAAGTGGTGGCAGATTAAGTCTCAGAAC
  TTTGATCTTGTCATCTGTTACAAGGTGGGGAAATTTTATGAGCTGTACCACATGGATGCT
  CTTATTGGAGTCAGTGAACTGGGGCTGGTATTCATGAAAGGCAACTGGGCCCATTCTGGC
  TTTCCTGAAATTGCATTTGGCCGTTATTCAGATTCCCTGGTGCAGAAGGGCTATAAAGTA
  GCACGAGTGGAACAGACTGAGACTCCAGAAATGATGGAGGCACGATGTAGAAAGATGGCA
  CATATATCCAAGTATGATAGAGTGGTGAGGAGGGAGATCTGTAGGATCATTACCAAGGGT
  ACACAGACTTACAGTGTGCTGGAAGGTGATCCCTCTGAGAACTACAGTAAGTATCTTCTT
  AGCCTCAAAGAAAAAGAGGAAGATTCTTCTGGCCATACTCGTGCATATGGTGTGTGCTTT
  GTTGATACTTCACTGGGAAAGTTTTTCATAGGTCAGTTTTCAGATGATCGCCATTGTTCG
  AGATTTAGGACTCTAGTGGCACACTATCCCCCAGTACAAGTTTTATTTGAAAAAGGAAAT
  CTCTCAAAGGAAACTAAAACAATTCTAAAGAGTTCATTGTCCTGTTCTCTTCAGGAAGGT
  CTGATACCCGGCTCCCAGTTTTGGGATGCATCCAAAACTTTGAGAACTCTCCTTGAGGAA
  GAATATTTTAGGGAAAAGCTAAGTGATGGCATTGGGGTGATGTTACCCCAGGTGCTTAAA
  GGTATGACTTCAGAGTCTGATTCCATTGGGTTGACACCAGGAGAGAAAAGTGAATTGGCC
  CTCTCTGCTCTAGGTGGTTGTGTCTTCTACCTCAAAAAATGCCTTATTGATCAGGAGCTT
  TTATCAATGGCTAATTTTGAAGAATATATTCCCTTGGATTCTGACACAGTCAGCACTACA
  AGATCTGGTGCTATCTTCACCAAAGCCTATCAACGAATGGTGCTAGATGCAGTGACATTA
  AACAACTTGGAGATTTTTCTGAATGGAACAAATGGTTCTACTGAAGGAACCCTACTAGAG
  AGGGTTGATACTTGCCATACTCCTTTTGGTAAGCGGCTCCTAAAGCAATGGCTTTGTGCC
  CCACTCTGTAACCATTATGCTATTAATGATCGTCTAGATGCCATAGAAGACCTCATGGTT
  GTGCCTGACAAAATCTCCGAAGTTGTAGAGCTTCTAAAGAAGCTTCCAGATCTTGAGAGG
  CTACTCAGTAAAATTCATAATGTTGGGTCTCCCCTGAAGAGTCAGAACCACCCAGACAGC
  AGGGCTATAATGTATGAAGAAACTACATACAGCAAGAAGAAGATTATTGATTTTCTTTCT
  GCTCTGGAAGGATTCAAAGTAATGTGTAAAATTATAGGGATCATGGAAGAAGTTGCTGAT
  GGTTTTAAGTCTAAAATCCTTAAGCAGGTCATCTCTCTGCAGACAAAAAATCCTGAAGGT
  CGTTTTCCTGATTTGACTGTAGAATTGAACCGATGGGATACAGCCTTTGACCATGAAAAG
  GCTCGAAAGACTGGACTTATTACTCCCAAAGCAGGCTTTGACTCTGATTATGACCAAGCT
  CTTGCTGACATAAGAGAAAATGAACAGAGCCTCCTGGAATACCTAGAGAAACAGCGCAAC
  AGAATTGGCTGTAGGACCATAGTCTATTGGGGGATTGGTAGGAACCGTTACCAGCTGGAA
  ATTCCTGAGAATTTCACCACTCGCAATTTGCCAGAAGAATACGAGTTGAAATCTACCAAG
  AAGGGCTGTAAACGATACTGGACCAAAACTATTGAAAAGAAGTTGGCTAATCTCATAAAT
  GCTGAAGAACGGAGGGATGTATCATTGAAGGACTGCATGCGGCGACTGTTCTATAACTTT
  GATAAAAATTACAAGGACTGGCAGTCTGCTGTAGAGTGTATCGCAGTGTTGGATGTTTTA
  CTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGGTCCTATGTGTCGCCCAGTAATTCTG
  TTGCCGGAAGATACCCCCCCCTTCTTAGAGCTTAAAGGATCACGCCATCCTTGCATTACG
  AAGACTTTTTTTGGAGATGATTTTATTCCTAATGACATTCTAATAGGCTGTGAGGAAGAG
  GAGCAGGAAAATGGCAAAGCCTATTGTGTGCTTGTTACTGGACCAAATATGGGGGGCAAG
  TCTACGCTTATGAGACAGGCTGGCTTATTAGCTGTAATGGCCCAGATGGGTTGTTACGTC
  CCTGCTGAAGTGTGCAGGCTCACACCAATTGATAGAGTGTTTACTAGACTTGGTGCCTCA
  GACAGAATAATGTCAGGTGAAAGTACATTTTTTGTTGAATTAAGTGAAACTGCCAGCATA
  CTCATGCATGCAACAGCACATTCTCTGGTGCTTGTGGATGAATTAGGAAGAGGTACTGCA
  ACATTTGATGGGACGGCAATAGCAAATGCAGTTGTTAAAGAACTTGCTGAGACTATAAAA
  TGTCGTACATTATTTTCAACTCACTACCATTCATTAGTAGAAGATTATTCTCAAAATGTT
  GCTGTGCGCCTAGGACATATGGCATGCATGGTAGAAAATGAATGTGAAGACCCCAGCCAG
  GAGACTATTACGTTCCTCTATAAATTCATTAAGGGAGCTTGTCCTAAAAGCTATGGCTTT
  AATGCAGCAAGGCTTGCTAATCTCCCAGAGGAAGTTATTCAAAAGGGACATAGAAAAGCA
  AGAGAATTTGAGAAGATGAATCAGTCACTACGATTATTTCGGGAAGTTTGCCTGGCTAGT
  GAAAGGTCAACTGTAGATGCTGAAGCTGTCCATAAATTGCTGACTTTGATTAAGGAATTA
  TAG
  
  Clone variation with respect to NM_000179.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000179 unedited
  TTGGATTTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGCGCGGTAGAT
  GCGGTGCTTTTAGGAGCTCCGTCCGACAGAACGGTTGGGCCTTGCCGGCTGTCGGTATGT
  CGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCCGGCGCTGAGTGATGCCAACA
  AGGCCTCGGCCAGGGCCTCACGCGAAGGCGGCCGTGCCGCCGCTGCCCCCGGGGCCTCTC
  CTTCCCCAGGCGGGGATGCGGCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTGGCGC
  GCTCCGCGTCACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTAGCGC
  CTGCTGCCCCCACCAGTTGTGACTTCTCACCAGGAGATTTGGTTTGGGCCAAGATGGAGG
  GTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTTTGATGGAACATTCATCCGCG
  AGAAAGGGAAATCAGTCCGTGTTCATGTACAGTTTTTTGATGACAGCCCAACAAGGGGCT
  GGGTTAGCAAAAGGCTTTTAAAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAGAAGG
  GAGGTCATTTTTACAGTGCANAGCCTGANATACTGAGAGCAATGCAACGTGCAGATGAAG
  CCTTAATAAAGACAGATTAAGAGGCTTNGATTGGCAGTTTGTGATGAGCCCTCAGAGCCA
  GAAGAGGAAGAAGAGATGGANGTAGGGCACACTTTACGTACAGATAAGAGTGAAGAAAGA
  TATGAAATTGAGAGTGAAGAGGAAGTACAGCCTAAGACACAGGGATCTAGCGAAGTAGCC
  GCCAAATAAAAAAACGAAGGGTCATATCAGATTCTGAGAGTGACATGGNTGGCTCTGATG
  TGGAATTTAAGCCCAGAACCTAAGGAGGAAAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000179 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTTTAAAAAATAAAG
  TTTATTAGATCATAATGTTGTCTGAATTTACCACCTTTGTCAGAAGTCAACTCAAAGCTT
  CCAATGTAGTCAGTCTATAATTCCTTAATCAAAGTCAGCAATTTATGGACAGCTTCAGCA
  TCTACAGTTGACCTTTCACTAGCCAGGCAAACTTCCCGAAATAATCGTAGTGACTGATTC
  ATCTTCTCAAATTCTCTTGCTTTTCTATGTCCCTTTTGAATAACTTCCTCTGGGAGATTA
  GCAAGCCTTGCTGCATTAAAGCCATAGCTTTTAGGACAAGCTCCCTTAATGAATTTATAG
  AGGAACGTAATAGTCTCCTGGCTGGGGTCTTCACATTCATTTTCTACCATGCATGCCATA
  TGTCCTAGGCGCACAGCAACATTTTGAGAATAATCTTCTACTAATGAATGGTAGTGAGTT
  GAAAATAATGTACGACATTTTATAGTCTCAGCAAGTTCTTTAACAACTGCATTTGCTATT
  GCCGTCCCATCAAATGTTGCAGTACCTCTTCCTAATTCATCCACAAGCACCAGAGAATGT
  GCTGTTGCATGCATGAGTATGCTGGCAGTTTCACTTAATTCAACAAAAAATGTACTTTCA
  CCTGACATTATTCTGTCTGAGGCACCAAGTCTAGTAAACACTCTATCAATTGGTGTGAGC
  CTGCACACTTCAGCAGGGACGTAACAACCCATCTGGGCCATTACAGCTAATAAGCCAGCC
  TGTCTCATAAGCGTAGACTTGCCCCNCATATTTGGTCCAGTAACAAGCACACATAGGGCT
  TGCCAATTTCCTGCTCCTCTTCTCACAGNCTATTAGAATGTCATTAGGATAAAATCATCT
  CCAAAAAAGTCTTGTATGCAGGATGCGGGATCTTTAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000179
• Insert Size: 4310 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000179.1, NP_000170.1
• RefSeq Size: 4264 bp
• RefSeq ORF: 4083 bp
• Locus ID: 2956
• UniProt ID: P52701
• Cytogenetics: 2p16.3
• Domains: PWWP, MutS_V, MutS_I, MutS_III, MutS_II, MutS_IV
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Colorectal cancer, Mismatch repair, Pathways in cancer
• Gene Summary: This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1).