PDE6 beta (PDE6B) (NM_000283) Human Untagged Clone

SKU SC120018

PDE6B (untagged)-Human phosphodiesterase 6B, cGMP-specific, rod, beta (PDE6B), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PDE6 beta
• Synonyms: CSNB3; CSNBAD2; GMP-PDEbeta; PDEB; rd1; RP40
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120018 sequence for NM_000283 edited (data generated by NextGen Sequencing); ATGAGCCTCAGTGAGGAGCAGGCCCGGAGCTTTCTGGACCAGAACCCCGATTTTGCCCGC; CAGTACTTTGGGAAGAAACTGAGCCCTGAGAATGTGGCCGCGGCCTGCGAGGACGGGTGC; CCGCCGGACTGCGACAGCCTCCGGGACCTCTGCCAGGTGGAGGAGAGCACGGCGCTGCTG; GAGCTGGTGCAGGATATGCAGGAGAGCATCAACATGGAGCGCGTGGTCTTCAAGGTCCTG; CGGCGCCTCTGCACCCTCCTGCAGGCCGACCGCTGCAGCCTCTTCATGTACCGCCAGCGC; AACGGCGTGGCCGAGCTGGCCACCAGGCTTTTCAGCGTGCAGCCGGACAGCGTCCTGGAG; GACTGCCTGGTGCCCCCCGACTCCGAGATCGTCTTCCCACTGGACATCGGGGTCGTGGGC; CACGTGGCTCAGACCAAAAAGATGGTGAACGTCGAGGACGTGGCCGAGTGCCCTCACTTC; AGCTCATTTGCTGACGAGCTCACTGACTACAAGACAAAGAATATGCTGGCCACACCCATC; ATGAATGGCAAAGACGTCGTGGCGGTGATCATGGCAGTGAACAAGCTCAACGGCCCATTC; TTCACCAGCGAAGACGAAGATGTGTTCTTGAAGTACCTGAATTTTGCCACGTTGTACCTG; AAGATCTATCACCTGAGCTACCTCCACAACTGCGAGACGCGCCGCGGCCAGGTGCTGCTG; TGGTCGGCCAACAAGGTGTTTGAGGAGCTGACGGACATCGAGAGGCAGTTCCACAAGGCC; TTCTACACGGTGCGGGCCTACCTCAACTGCGAGCGGTACTCCGTGGGCCTCCTGGACATG; ACCAAGGAGAAGGAATTTTTTGACGTGTGGTCTGTGCTGATGGGAGAGTCCCAGCCGTAC; TCGGGCCCACGCACGCCTGATGGCCGGGAAATTGTCTTCTACAAAGTGATCGACTACATC; CTCCACGGCAAGGAGGAGATCAAGGTCATTCCCACACCCTCAGCCGATCACTGGGCCCTG; GCCAGCGGCCTTCCAAGCTACGTGGCAGAAAGCGGCTTTATTTGTAACATCATGAATGCT; TCCGCTGACGAAATGTTCAAATTTCAGGAAGGGGCCCTGGACGACTCCGGGTGGCTCATC; AAGAATGTGCTGTCCATGCCCATCGTCAACAAGAAGGAGGAGATTGTGGGAGTCGCCACA; TTTTACAACAGGAAAGACGGGAAGCCCTTTGACGAACAGGACGAGGTTCTCATGGAGTCC; CTGACACAGTTCCTGGGCTGGTCAGTGATGAACACCGACACCTACGACAAGATGAACAAG; CTGGAGAACCGCAAGGACATCGCACAGGACATGGTCCTTTACCACGTGAAGTGCGACAGG; GACGAGATCCAGCTCATCCTGCCAACCAGAGCGCGCCTGGGGAAGGAGCCTGCTGACTGC; GATGAGGACGAGCTGGGCGAAATCCTGAAGGAGGAGCTGCCAGGGCCCACCACATTTGAC; ATCTACGAATTCCACTTCTCTGACCTGGAGTGCACCGAACTGGACCTGGTCAAATGTGGC; ATCCAGATGTACTACGAGCTGGGCGTGGTCCGAAAGTTCCAGATCCCCCAGGAGGTCCTG; GTGCGGTTCCTGTTCTCCATCAGCAAAGGGTACCGGAGAATCACCTACCACAACTGGCGC; CACGGCTTCAACGTGGCCCAGACGATGTTCACGCTGCTCATGACCGGCAAACTGAAGAGC; TACTACACGGACCTGGAGGCCTTCGCCATGGTGACAGCCGGCCTGTGCCATGACATCGAC; CACCGCGGCACCAACAACCTGTACCAGATGAAGTCCCAGAACCCCTTGGCTAAGCTCCAC; GGCTCCTCGATTTTGGAGCGGCACCACCTGGAGTTTGGGAAGTTCCTGCTCTCGGAGGAG; ACCCTGAACATCTACCAGAACCTGAACCGGCGGCAGCACGATCACGTGATCCACCTGATG; GACATCGCCATCATCGCCACGGACCTGGCCCTGTACTTCAAGAAGAGAGCGATGTTTCAG; AAGATCGTGGATGAGTCCAAGAACTACCAGGACAAGAAGAGCTGGGTGGAGTACCTGTCC; CTGGAGACGACCCGGAAGGAGATCGTCATGGCCATGATGATGACAGCCTGCGACCTGTCT; GCCATCACCAAGCCCTGGGAAGTCCAGAGCAAGGTCGCACTTCTCGTGGCTGCTGAGTTC; TGGGAGCAAGGTGACTTGGAAAGGACAGTCTTGGATCAGCAGCCCATTCCTATGATGGAC; CGGAACAAGGCGGCCGAGCTCCCCAAGCTGCAAGTGGGCTTCATCGACTTCGTGTGCACA; TTCGTGTACAAGGAGTTCTCTCGTTTCCACGAAGAGATCCTGCCCATGTTCGACCGACTG; CAGAACAATAGGAAAGAGTGGAAGGCGCTGGCTGATGAGTATGAGGCCAAAGTGAAGGCT; CTGGAGGAGAAGGAGGAGGAGGAGAGGGTGGCAGCCAAGAAANNAGGCACAGAAATTTGC; AATGGCGGCCCAGCACCCAAGTCTTCAACCTGCTGTATCCTGTGA; ; Clone variation with respect to NM_000283.3; 1962 g=>t;2503 g=>n;2504 t=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000283 unedited; GCCGCNCCTTGGTTTNTTTAAACGATTCATATAGGCGGCGCGCGAATTCGCACGAGGTGC; GTGCCTGGCACAGCAGCGTCTCCAGGGCAGGCAGCCACCATGAGCCTCAGTGAGGAGCAG; GCCCGGAGCTTTCTGGACCAGAACCCCGATTTTGCCCGCCAGTACTTTGGGAAGAAACTG; AGCCCTGAGAATGTGGCCGCGGCCTGCGAGGACGGGTGCCCGCCGGACTGCGACAGCCTC; CGGGACCTCTGCCAGGTGGAGGAGAGCACGGCGCTGCTGGAGCTGGTGCAGGATATGCAG; GAGAGCATCAACATGGAGCGCGTGGTCTTCAAGGTCCTGCGGCGCCTCTGCACCCTCCTG; CAGGCCGACCGCTGCAGCCTCTTCATGTACCGCCAGCGCAACGGCGTGGCCGAGCTGGCC; ACCAGGCTTTTCAGCGTGCAGCCGGACAGCGTCCTGGAGGACTGCCTGGTGCCCCCCGAC; TCCGAGATCGTCTTCCCACTGGACATCGGGGTCGTGGGCCACGTGGCTCAGACCAAAAAG; ATGGTGAACGTCGAGGACGTGGCCGAGTGCCCTCACTTCAGCTCATTTGCTGACGAGCTC; ACTGACTACAAGACAAAGAATATGCTGGCCACACCCATCATGAATGGCAAAGACGTCGTG; GCGGTGATCATGGCAGTGAACAAGCTCAACGGCCCATTCTTCACCAGCGAAGACGAAGAT; GTGTTCTTGAAGTACCTGAATTTTGCCACGTTGTACCTGGAGATCTATCACCTGAGCTAC; CTNCACAACTGCGAGACGCGCCGCGGCCCAGTGCTGCTGTGGGTCGCCAACAAGGTGTTT; TGAGAGCTGACGGACATCGAGAGGCAGTTCCACAGGCCTTCTACACGGTG;
• Restriction Sites: Please inquire
• ACCN: NM_000283
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000283.1, NP_000274.1
• RefSeq Size: 3231 bp
• RefSeq ORF: 2565 bp
• Locus ID: 5158
• UniProt ID: P35913
• Cytogenetics: 4p16.3
• Domains: GAF, HDc, PDEase
• Protein Families: Druggable Genome
• Summary: Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).