ornithine aminotransferase (OAT) (NM_000274) Human Untagged Clone

SKU SC120012

OAT (untagged)-Human ornithine aminotransferase (OAT), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ornithine aminotransferase
• Synonyms: GACR; HOGA; OATASE; OKT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120012 sequence for NM_000274 edited (data generated by NextGen Sequencing); ATGTTTTCCAAACTAGCACATTTGCAGAGGTTTGCTGTACTTAGTCGCGGAGTTCATTCT; TCAGTGGCTTCTGCTACATCTGTTGCAACTAAAAAAACAGTCCAAGGCCCTCCAACCTCT; GATGACATTTTTGAAAGGGAATATAAGTATGGTGCACACAACTACCATCCTTTACCTGTA; GCCCTGGAGAGAGGAAAAGGTATTTACTTATGGGATGTAGAAGGCAGAAAATATTTTGAC; TTCCTGAGTTCTTACAGTGCTGTCAACCAAGGGCATTGTCACCCCAAGATTGTGAATGCT; CTGAAGAGTCAAGTGGACAAATTGACCTTAACATCTAGAGCTTTCTATAATAACGTACTT; GGTGAATATGAGGAGTATATTACTAAACTTTTCAACTACCACAAAGTTCTTCCTATGAAT; ACAGGAGTGGAGGCTGGAGAGACTGCCTGTAAACTAGCTCGTAAGTGGGGCTATACCGTG; AAGGGCATTCAGAAATACAAAGCAAAGATTGTTTTTGCAGCTGGGAACTTCTGGGGTAGG; ACGTTGTCTGCTATCTCCAGTTCCACAGACCCAACCAGTTACGATGGTTTTGGACCATTT; ATGCCGGGATTCGACATCATTCCCTATAATGATCTGCCCGCACTGGAGCGTGCTCTTCAG; GATCCAAATGTGGCTGCGTTCATGGTAGAACCAATTCAGGGTGAAGCAGGCGTTGTTGTT; CCGGATCCAGGTTACCTAATGGGAGTGCGAGAGCTCTGCACCAGGCACCAGGTTCTCTTT; ATTGCTGATGAAATACAGACAGGATTGGCCAGAACTGGTAGATGGCTGGCTGTTGATTAT; GAAAATGTCAGACCTGATATAGTCCTCCTTGGAAAGGCCCTTTCTGGGGGCTTATACCCT; GTGTCTGCAGTGCTGTGTGATGATGACATCATGCTGACCATTAAGCCAGGGGAGCATGGG; TCCACATACGGTGGCAATCCACTAGGCTGCCGAGTGGCCATCGCAGCCCTTGAGGTTTTA; GAAGAAGAAAACCTTGCTGAAAATGCAGACAAATTGGGCATTATCTTGAGAAATGAACTC; ATGAAGCTACCTTCTGATGTTGTAACTGCCGTAAGAGGAAAAGGATTATTAAATGCTATT; GTCATTAAAGAAACCAAAGATTGGGATGCTTGGAAGGTGTGTCTACGACTTCGAGATAAT; GGACTTCTGGCCAAGCCAACCCATGGCGACATTATCAGGTTTGCGCCTCCGCTGGTGATC; AAGGAGGATGAGCTTCGAGAGTCCATTGAAATTATTAACAAGACCATCTTGTCTTTCTGA; ; Clone variation with respect to NM_000274.3; 1134 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000274 unedited; ACTAATTTGTAATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCCGGTTGT; CCTCAGGCGCTGTCAGATCTGTGGTTTTTCTACTTGAAGGACACAATGTTTTCCAAACTA; GCACATTTGCAGAGGTTTGCTGTACTTAGTCGCGGAGTTCATTCTTCAGTGGCTTCTGCT; ACATCTGTTGCAACTAAAAAACAGTCCAAGGCCCTCCAACCTCTGATGACATTTTTGAAA; GGGAATATAAGTATGGTGCACACAACTACCATCCTTTACCTGTAGCCCTGGAGAGAGGAA; AAGGTATTTACTTATGGGATGTAGAAGGCAGAAAATATTTTGACTTCCTGAGTTCTTACA; GTGCTGTCAACCAAGGGCATTGTCACCCCAAGATTGTGAATGCTCTGAAGAGTCAAGTGG; ACAAATTGACCTTAACATCTAGAGCTTTCTATAATAACGTACTTGGTGAATATGAGGAGT; ATATTACTAAACTTTTCAACTACCACAAAGTTCTTCCTATGAATACAGGAGTGGAGGCTG; GAGAGACTGCCTGTAAACTAGCTCGTAAGTGGGGCTATACCGTGAAGGGCATTCAGAAAT; ACAAAGCACAGATTGTTTTTGCAGCTGGGAACTTCTGGGGTAGGACGTTGTCTGCTATCT; CCAGTTCCACAGACCCAACCAGTTACGATGGTTTTGGACCATTTATGCCCGGATTCGACA; TCATTCCCTCATATGATCTTGCCGCACCTGGACGTGCTCTCTACGATCCAAAAGTGGCTG; CGTTCATGGTAGAACCATTCAGGGTGAAACAGGCGTTGTTGCTCCGGACCAGTTACCTAA; TGGGGTGCGAGAGCTCTGCCCAGGCACCAGGTCTCTTATGCTGCGAATACAACAGAATGC; CAAACCGGGAAAGCTGCTGCGATCATAAAGGTCCACTGAATACCCTCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000274 unedited; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGACATTTGAAATATAAGCT; TTATTTTAAATTTAAAGAAGTATTGAAAATAAACATTTTTTACAAATTATAATCAAGCAC; TCAAAACAATTTAGGAATGTTAAACACTAATTCTTAATTCAAAATAATGACATCCATAGA; ATACACTCCTGGTGTTGGCCAATATGAAGTTTACTTAATATTAGTATTTTATATACACTT; AACCATTAATCCTTCCTAAAATTCAATAACAATGATTTCACTTTATAAGATGAAGCCTTT; TATGCAATACCCAGAGATAACTTTTTCAAATATGAAACACTTATACCAGTGAGGAAATTA; TAAAAACATATATCAATTATACTGAAGGACTTGATTTAGAGGCTGTCTGTATATAGATGC; ATTTCACCTTAGGAAGTACACATGCACATCAAAACACTTCAACTGAATATAGATGCCATT; ACATTATTTAGTTACGTTACAAAGCAAACGGCAGGTTCATAAACGTTGTTCTATTATGTA; TCAACTGAAAAAATATATTCAAAAAAAAAGTTTTTGAAGACTCATGGGAGTGGAATGTGC; CCACATTANGAATAAAGCTTTTACAGGACCACCTGTCTCCAGCTGGCTCCAGGGACCACT; GAAAACAGCTGGCTACCCTCAGAAAGACAGATGGCCTTGTTAATAATTTCATGGACTCTC; GAAGCTCATCCTCCTTGATCACCAACGGAGGCGCAACCTGATTATGTCGNCATGGGTTGG; TTTGCCAGAAGTCCATATTCTCGAGTCGAAAAACACCTTCCAGCATCCCATTTTTGTTTT; CTTATGACAAACCATTAAAAACCTTTTCTTTTACGGAATTACAACTCGGAGGTACCTCCT; GAGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000274
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000274.1, NP_000265.1
• RefSeq Size: 2013 bp
• RefSeq ORF: 1320 bp
• Locus ID: 4942
• UniProt ID: P04181
• Cytogenetics: 10q26.13
• Domains: aminotran_3
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Summary: This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).