Phospholipase A2 IIA (PLA2G2A) (NM_000300) Human Untagged Clone

SKU SC119954

PLA2G2A (untagged)-Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Phospholipase A2 IIA
• Synonyms: MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000300 edited; ATGAAGACCCTCCTACTGTTGGCAGTGATCATGATCTTTGGCCTACTGCAGGCCCATGGG; AATTTGGTGAATTTCCACAGAATGATCAAGTTGACGACAGGAAAGGAAGCCGCACTCAGT; TATGGCTTCTACGGCTGCCACTGTGGCGTGGGTGGCAGAGGATCCCCCAAGGATGCAACG; GATCGCTGCTGTGTCACTCATGACTGTTGCTACAAACGTCTGGAGAAACGTGGATGTGGC; ACCAAATTTCTGAGCTACAAGTTTAGCAACTCGGGGAGCAGAATCACCTGTGCAAAACAG; GACTCCTGCAGAAGTCAACTGTGTGAGTGTGATAAGGCTGCTGCCACCTGTTTTGCTAGA; AACAAGACGACCTACAATAAAAAGTACCAGTACTATTCCAATAAACACTGCAGAGGGAGC; ACCCCTCGTTGCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000300 unedited; AGACGACTAGGTTTGNATACNACTTCTATGGGCGGCCGAGCACTTCGCACCAGGGGGATA; CAACTTTGGAGTCCTCTGAGAGAGCCACCAAGNAGGAGCAGGGGAGCGACGGCCGGGGCA; GAAGTTGAGACCACCCAGCAGAGGAGCTAGGCCAGTCCATCTGCATTTGTCACCCAAGAA; CTCTTACCATGAAGACCCTCCTACTGTTGGCAGTGATCTTGATCTTTGGCCTACTGCAGG; CCCATGGGAATTTGGTGAATTTCCACAGAATGATCAAGTTGACGACAGGAAAGGAAGCCG; CACTCAGTTATGGCTTCTACGGCTGCCACTGTGGCGTGGGTGGCAGAGGATCCCCCAAGG; ATGCAACGGATCGCTGCTGTGTCACTCATGACTGTTGCTACAAACGTCTGGAGAAACGTG; GATGTGGCACCAAATTTCTGAGCTACAAGTTTAGCAACTCGGGGAGCAGAATCACCTGTG; CAAAACAGGACTCCTGCAGAAGTCAACTGTGTGAGTGTGATAAGGCTGCTGCCACCTGTT; TTGCTAGAAACAAGACGACCTACAATAAAAAGTACCAGTACTATTCCAATAAACACTGCA; GAGGGAGCACCCCTCGTTGCTGAGTCCCCTCTTCCCTGGAAACCTTCCACCCAGTGCTGA; ATTTCCCTCTCTCATACCCTCCCTCCCTACCCTAACCAAGTTCCTTGGCCATGCAGAAAG; CATCCCTCACCCATCCTAGAGGCCAGGCAGGAGCCCTTCTATACCCACCCAGAATGAGAC; ATCCAGCAGATTTCCAGCCTTCTACTGCTCTCCTCCACCTCAACTCCGTGCTTAACCAAA; GAAGCTGTACTCCGGGGGGTCTCTTCTGAATAAGCATTAGCAATCAAAAAAAAAAAAAAA; ACTCGCTCTAGATTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000300 unedited; GACCGCGCCGCAATCTANAGTCGAGTTTTTTTTTTTNTTTTTTGTTTGCTAATTGCTTTA; TTAAAAAGACCCCCCGGATACAGCTTCTTTGGTTAAGCACGGAGTTGAGGTGGAGGAGAG; CAGTAGAAGGCTGGAAATCTGCTGGATGTCTCATTCTGGGTGGGTATAGAAGGGCTCCTG; CCTGGCCTCTAGGATGGGTGAGGGATGCTTTCTGCATGGCCAAGGAACTTGGTTAGGGTA; GGGAGGGAGGGTATGAGAGAGGGAAATTCATCACTGGGTGGAAGGTTTCCAGGGAAGAGG; GGACTCACCACCTACGGGTGCTCCCTCTGCACCGCCCATCGGAAACTTCCTGGTACTTTT; TTTTGTACGTCTTTTTGTTACTTACACAACCCCTCCCCTCATCCCTCTTAAACCCCCCTT; CGTTGACCTTCCCCCGTACCCTTTTCTTCCTACGTCATTCATGCTCCCCCCCTTGTTCAC; CTTGAATCCCCCCAATACTCCCCCTCTTCCCCCCTTTTCCCCCCCTCTTCCCCTTCCCCC; CACTCCTTTCCCTCCCTCCCCCCTCCCTTCTCTTTTCTCCTCTTCCCCCCCTCTCTACCA; CTTCCCTCCCCCCACCCTCCCCNCTTTTCTTCCCTCTTTTCCTCCCCCCTCTCTTTTCTC; CTTCTTTCCCCCCCCCCTCTCCCCCCCCCCTCCTTTTCTTCTCCCCCCCCTCTCCCTTCC; CCTTTCCTTTTTTCCTTCCCCCTTTTCCNCTTCCCTCCCCCCCNNTTCCCTTTCTCCCCT; TCCCCTCCCCCCCCCCCTCCTTCTTTCCCCCCCCCCCTCCCTTTCCCCATTCTCCATCTC; CCCGTTCTTCTTCCCCCCTTCTCCCGCCCTCTTTCTCCCTTCAGACCCTTTCCTCTCCTC; CCCCTTTTCGTACGCTCCCCTCCCTTTTCCTCCCCCGCTATCCCTCCCCTTTTCTCTACT; TTCTCCCCCCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000300
• Insert Size: 910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000300.2, NP_000291.1
• RefSeq Size: 997 bp
• RefSeq ORF: 435 bp
• Locus ID: 5320
• UniProt ID: P14555
• Cytogenetics: 1p36.13
• Domains: PA2c
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Summary: The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]; Transcript Variant: This variant (1) represents the predominant and longest transcript. Variants 1-4 encode the same protein.