p21(CDKN1A) (NM_000389) Human Untagged Clone

CAT#: SC119947

CDKN1A (untagged)-Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1

USD 196.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name p21(CDKN1A) (NM_000389) Human Untagged Clone
Symbol CDKN1A
Synonyms CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1
Vector pCMV6-XL5
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_000389 edited
ATGTCAGAACCGGCTGGGGATGTCCGTCAGAACCCATGCGGCAGCAAGGCCTGCCGCCGC
CTCTTCGGCCCAGTGGACAGCGAGCAGCTGAGCCGCGACTGTGATGCGCTAATGGCGGGC
TGCATCCAGGAGGCCCGTGAGCGATGGAACTTCGACTTTGTCACCGAGACACCACTGGAG
GGTGACTTCGCCTGGGAGCGTGTGCGGGGCCTTGGCCTGCCCAAGCTCTACCTTCCCACG
GGGCCCCGGCGAGGCCGGGATGAGTTGGGAGGAGGCAGGCGGCCTGGCACCTCACCTGCT
CTGCTGCAGGGGACAGCAGAGGAAGACCATGTGGACCTGTCACTGTCTTGTACCCTTGTG
CCTCGCTCAGGGGAGCAGGCTGAAGGGTCCCCAGGTGGACCTGGAGACTCTCAGGGTCGA
AAACGGCGGCAGACCAGCATGACAGATTTCTACCACTCCAAACGCCGGCTGATCTTCTCC
AAGAGGAAGCCCTAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000389 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCGAAGTCAGTTCC
TTGTGGAGCCGGAGCTGGGCGCGGATTCGCCGAGGCACCGAGGCACTCAGAGGAGGCGCC
ATGTCAGAACCGGCTGGGGATGTCCGTCAGAACCCATGCGGCAGCAAGGCCTGCCGCCGC
CTCTTCGGCCCAGTGGACAGCGAGCAGCTGAGCCGCGACTGTGATGCGCTAATGGCGGGC
TGCATCCAGGAGGCCCGTGAGCGATGGAACTTCGACTTTGTCACCGAGACACCACTGGAG
GGTGACTTCGCCTGGGAGCGTGTGCGGGGCCTTGGCCTGCCCAAGCTCTACCTTCCCACG
GGGCCCCGGCGAGGCCGGGATGAGTTGGGAGGAGGCAGGCGGCCTGGCACCTCACCTGCT
CTGCTGCAGGGGACAGCAGAGGAAGACCATGTGGACCTGTCACTGTCTTGTACCCTTGTG
CCTCGCTCAGGGGAGCAGGCTGAAGGGTCCCCAGGTGGACCTGGAGACTCTCAGGGTCGA
AAACGGCGGCAGACCAGCATGACAGATTTCTACCACTCCAAACGCCGGCTGATCTTCTCC
AAGAGGAAGCCCTAATCCGCCCACAGGAAGCCTGCAGTCCTGGAAGCGCGAGGGCCTCAA
AGGCCCGCTCTACATCTTCTGCCTTAGTCTCAGTTTGTGTGTCTTAATTATTATTTGTGT
TTTAATTTAAACACCTCCTCATGTACATACCCTGGCCGCCCCCTGCCCCCCAGCCTCTGG
CATTAGAATTATNTAAACAANAACTANGCGGTTGAATGAGAAGTTCCTAAGAGTGCNTGG
CATTNTTATTTTATGAAATACTATTTAAAGCCTNCTCANCCCGTGNTCTCCTTTCCTCTC
TCCCGGAGGTGGGGTGGGCCGGCTCATGCCAGCTACTTNCTCCTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000389 unedited
CGCGGCACGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTAAAGGCACTAAAAATCATTTATTGAGCACCTGCTGTATATTCAGCATTGGGGGAGGAG
CTGTGAAAGACACAAAACAGTACAGGGTGGGGTCCCTGCCCTCGAGAGGTTTACAGTCTA
GGGGGAGAAACGGGAACCAGGACACATGGGGAGCCGAGAGAAAACAGTCCAGGCCAGTAT
GTTACAGGAGCTGGAAGGTGTTTGGGGTCAAACCCCAATACTCCAAGTACACTAAGCACT
TCAGTGCCTCCAGGGGCTCAACGTTAGTGCCAGGAAAGACAACTACTCCCAGCCCCATAT
GAGCCCACGTGGCATGCCCTGTCCATAGCCTTTACTGCCACCATCTTAAAATGTCTGACT
CCTTGTTCCGCTGCTAATCAAAGTGCAATGAACTGGGGAGGGATGGGGTGGATGAAGAAG
GTCGCTGGACAATTTGAGGGGCCAGTGTCTCCCTCCTAAAAAGATCTACTCCCCCATCAT
ATACCCCTAACACAGAGATAACCCCACTCAAGGGGGCCTGTGCCACCACATGGGACCCTC
ACCCCCACAGTTAGAGGAGGGGGCGGCCGCTGCTTGAGCTGCCTGAGGTAAAACTAGGGT
GCCCTTCTTCTTGTGTGTCCCTTCCCCTTCAGTCCATTGAGCTGGGGGTGGGGGTGGGAC
AGGCACCTCAGAGCCATCTGGAGCTGATAGGGTCCTGAAGGGAAACGACCAGGGGGAGGA
ACAGCACCAAAGGGGATTTGCCNAGCCCATCTGGAAAATATGTTTACCTGGGCCTCCTCA
AAAGGTCAGGGGAGCCAAAAAGGAAATAGCTAACACTGAGACGCCTCNCCAGTGCAGGTA
AAAGGCCCATGAGGCAGCGGGGTGGTTTGTTCAGGACCCACCTTCCCCTGCCTTAAAGAA
CAAGGAG
Restriction Sites NotI-NotI     
Tag Tag Free
ACCN NM_000389
ORF Size 495 bp
Insert Size 2100
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reference Data
RefSeq NM_000389.2, NP_000380
RefSeq Size 2140
Locus ID 1026
Cytogenetics 6p21.2
Domains CDI
Protein Families Druggable Genome
Protein Pathways ErbB signaling pathway, Cell cycle, p53 signaling pathway, Pathways in cancer, Glioma, Prostate cancer, Melanoma, Bladder cancer, Chronic myeloid leukemia
Gene Summary This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015].
Transcript Variant: This variant (1) differs in the 5' UTR, lacks an in-frame portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 3. The resulting isoform (1) has a shorter N-terminus, compared to isoform 2. Variants 1, 2, 4 and 5 encode the same isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations