Calcium Sensing Receptor (CASR) (NM_000388) Human Untagged Clone

SKU SC119946

CASR (untagged)-Human calcium-sensing receptor (CASR), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Calcium Sensing Receptor
• Synonyms: CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC; HHC1; HYPOC1; NSHPT; PCAR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119946 sequence for NM_000388 edited (data generated by NextGen Sequencing); ATGGCATTTTATAGCTGCTGCTGGGTCCTCTTGGCACTCACCTGGCACACCTCTGCCTAC; GGGCCAGACCAGCGAGCCCAAAAGAAGGGGGACATTATCCTTGGGGGGCTCTTTCCTATT; CATTTTGGAGTAGCAGCTAAAGATCAAGATCTCAAATCAAGGCCGGAGTCTGTGGAATGT; ATCAGGTATAATTTCCGTGGGTTTCGCTGGTTACAGGCTATGATATTTGCCATAGAGGAG; ATAAACAGCAGCCCAGCCCTTCTTCCCAACTTGACGCTGGGATACAGGATATTTGACACT; TGCAACACCGTTTCTAAGGCCTTGGAAGCCACCCTGAGTTTTGTTGCTCAAAACAAAATT; GATTCTTTGAACCTTGATGAGTTCTGCAACTGCTCAGAGCACATTCCCTCTACGATTGCT; GTGGTGGGAGCAACTGGCTCAGGCGTCTCCACGGCAGTGGCAAATCTGCTGGGGCTCTTC; TACATTCCCCAGGTCAGTTATGCCTCCTCCAGCAGACTCCTCAGCAACAAGAATCAATTC; AAGTCTTTCCTCCGAACCATCCCCAATGATGAGCACCAGGCCACTGCCATGGCAGACATC; ATCGAGTATTTCCGCTGGAACTGGGTGGGCACAATTGCAGCTGATGACGACTATGGGCGG; CCGGGGATTGAGAAATTCCGAGAGGAAGCTGAGGAAAGGGATATCTGCATCGACTTCAGT; GAACTCATCTCCCAGTACTCTGATGAGGAAGAGATCCAGCATGTGGTAGAGGTGATTCAA; AATTCCACGGCCAAAGTCATCGTGGTTTTCTCCAGTGGCCCAGATCTTGAGCCCCTCATC; AAGGAGATTGTCCGGCGCAATATCACGGGCAAGATCTGGCTGGCCAGCGAGGCCTGGGCC; AGCTCCTCCCTGATCGCCATGCCTCAGTACTTCCACGTGGTTGGCGGCACCATTGGATTC; GCTCTGAAGGCTGGGCAGATCCCAGGCTTCCGGGAATTCCTGAAGAAGGTCCATCCCAGG; AAGTCTGTCCACAATGGTTTTGCCAAGGAGTTTTGGGAAGAAACATTTAACTGCCACCTC; CAAGAAGGTGCAAAAGGACCTTTACCTGTGGACACCTTTCTGAGAGGTCACGAAGAAAGT; GGCGACAGGTTTAGCAACAGCTCGACAGCCTTCCGACCCCTCTGTACAGGGGATGAGAAC; ATCAGCAGTGTCGAGACCCCTTACATAGATTACACGCATTTACGGATATCCTACAATGTG; TACTTAGCAGTCTACTCCATTGCCCACGCCTTGCAAGATATATATACCTGCTTACCTGGG; AGAGGGCTCTTCACCAATGGCTCCTGTGCAGACATCAAGAAAGTTGAGGCGTGGCAGGTC; CTGAAGCACCTACGGCATCTAAACTTTACAAACAATATGGGGGAGCAGGTGACCTTTGAT; GAGTGTGGTGACCTGGTGGGGAACTATTCCATCATCAACTGGCACCTCTCCCCAGAGGAT; GGCTCCATCGTGTTTAAGGAAGTCGGGTATTACAACGTCTATGCCAAGAAGGGAGAAAGA; CTCTTCATCAACGAGGAGAAAATCCTGTGGAGTGGGTTCTCCAGGGAGGTGCCCTTCTCC; AACTGCAGCCGAGACTGCCTGGCAGGGACCAGGAAAGGGATCATTGAGGGGGAGCCCACC; TGCTGCTTTGAGTGTGTGGAGTGTCCTGATGGGGAGTATAGTGATGAGACAGATGCCAGT; GCCTGTAACAAGTGCCCAGATGACTTCTGGTCCAATGAGAACCACACCTCCTGCATTGCC; AAGGAGATCGAGTTTCTGTCGTGGACGGAGCCCTTTGGGATCGCACTCACCCTCTTTGCC; GTGCTGGGCATTTTCCTGACAGCCTTTGTGCTGGGTGTGTTTATCAAGTTCCGCAACACA; CCCATTGTCAAGGCCACCAACCGAGAGCTCTCCTACCTCCTCCTCTTCTCCCTGCTCTGC; TGCTTCTCCAGCTCCCTGTTCTTCATCGGGGAGCCCCAGGACTGGACGTGCCGCCTGCGC; CAGCCGGCCTTTGGCATCAGCTTCGTGCTCTGCATCTCATGCATCCTGGTGAAAACCAAC; CGTGTCCTCCTGGTGTTTGAGGCCAAGATCCCCACCAGCTTCCACCGCAAGTGGTGGGGG; CTCAACCTGCAGTTCCTGCTGGTTTTCCTCTGCACCTTCATGCAGATTGTCATCTGTGTG; ATCTGGCTCTACACCGCGCCCCCCTCAAGCTACCGCAACCAGGAGCTGGAGGATGAGATC; ATCTTCATCACGTGCCACGAGGGCTCCCTCATGGCCCTGGGCTTCCTGATCGGCTACACC; TGCCTGCTGGCTGCCATCTGCTTCTTCTTTGCCTTCAAGTCCCGGAAGCTGCCGGAGAAC; TTCAATGAAGCCAAGTTCATCACCTTCAGCATGCTCATCTTCTTCATCGTCTGGATCTCC; TTCATTCCAGCCTATGCCAGCACCTATGGCAAGTTTGTCTCTGCCGTAGAGGTGATTGCC; ATCCTGGCAGCCAGCTTTGGCTTGCTGGCGTGCATCTTCTTCAACAAGATCTACATCATT; CTCTTCAAGCCATCCCGCAACACCATCGAGGAGGTGCGTTGCAGCACCGCAGCTCACGCT; TTCAAGGTGGCTGCCCGGGCCACGCTGCGCCGCAGCAACGTCTCCCGCAAGCGGTCCAGC; AGCCTTGGAGGCTCCACGGGATCCACCCCCTCCTCCTCCATCAGCAGCAAGAGCAACAGC; GAAGACCCATTCCCACAGCCCGAGAGGCAGAAGCAGCAGCAGCCGCTGGCCCTAACCCAG; CAAGAGCAGCAGCAGCAGCCCCTGACCCTCCCACAGCAGCAACGATCTCAGCAGCAGCCC; AGATGCAAGCAGAAGGTCATCTTTGGCAGCGGCACGGTCACCTTCTCACTGAGCTTTGAT; GAGCCTCAGAAGAACGCCATGGCCCACAGGAATTCTACGCACCAGAACTCCCTGGAGGCC; CAGAAAAGCAGCGATACGCTGACCCGACACCAGCCATTACTCCCGCTGCAGTGCGGGGAA; ACGGACTTAGATCTGACCGTCCAGGAAACAGGTCTGCAAGGACCTGTGGGTGGAGACCAG; CGGCCAGAGGTGGAGGACCCTGAAGAGTTGTCCCCAGCACTTGTAGTGTCCAGTTCACAG; AGCTTTGTCATCAGTGGTGGAGGCAGCACTGTTACAGAAAACGTAGTGAATTCATAA; ; Clone variation with respect to NM_000388.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000388 unedited; CCATTCAGATTTGTATACGATTCATATAGGCGGCCTGCGAATTCGCACCAGCTTTCGCGC; TAGAGAGTGGAAGGAGGGAGCTGTTTGCCAGCACCGAGGTCTTGCGGCACAGGCAACGCT; TGACCTGAGTCTTGCAGAATGAAAGGCATCACAGGAGGCCTCTGCATGATGTGGCTTCCA; AAGACTCAAGGACCACCCACATTACAAGTCTGGATTGAGGAAGGCAGAAATGGAGATTCA; AACACCACGTCTTCTATTATTTTATTAATCAATCTGTAGACATGTGTCCCCACTGCAGGG; AGTGAACTGCTCCAAGGGAGAAACTTCTGGGAGCCTCCAAACTCCTAGCTGTCTCATCCC; TTGCCCTGGAGAGACGGCAGAACCATGGCATTTTATAGCTGCTGCTGGGTCCTCTTGGCA; CTCACCTGGCACACCTCTGCCTACGGGCCAGACCAGCGAGCCCAAAAGAAGGGGGACATT; ATCCTTGGGGGGCTCTTTCCTATTCATTTTGGAGTAGCAGCTAAAGATCAAGATCTCAAA; TCAAGGCCGGAGTCTGTGGAATGTATCAGGTATAATTTCCGTGGGTTTCGCTGGTTACAG; GCTATGATATTTGCCATAGAGGAGATAAACAGCAGCCCAGCCCTTCTTCCACACTTGACG; CTGGGATACAGGATATTTGACACTTGCAACACCGTTTCTAAGGCCTTGGAAGCCACCCTG; AGTTTTGTTGCTCAGAACAAAATTGATTCTTTGAACCTTGATGAGTTCTGCAACTGCTCA; GAGCACATTCCCTCTACGATTGCTGTGGTGGGAGCAACTGGCTCAGGCGTCTCCCGGCAG; TGGCACTCTGCTGGNGCTCTTCTACATCCCCNAGTCAGACTTGCTNCTNCAGCAGATNCT; CAGCACTAGATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000388 unedited; AAATACGTTGAACCGCGGCCCGCTATCTACGAGCGNGGNATTTTTTTTTTTTTTTTTTGC; TCTTCTTTTTTTTTAATTTTAAAGGGAACATGGGTCAATTCACTGTCATTTAAGATGGTG; TGACTAAATTCGGGGCATTTGATGTGTCTATTATCCCTTCTTCCTCAGAGGAAAGGAGTC; TGGGGCGATTCCTCATCCCTGGGACCCCAAGAAACCTCTCTGCATTCTCCCTAGCCCAGG; CTTCTCCTTCCATTTTATGAATTCACTACGTTTTCTGTAACAGTGCTGCCTCCACCACTG; ATGACAAAGCTCTGTGAACTGGACACTACAAGTGCTGGGGACAACTCTTCAGGGTCCTCC; ACCTCTGGCCGCTGGTCTCCACCCACAGGTCCTTGCAGACCTGTTTCCTGGACGGTCAGA; TCTAAGTCCGTTTCCCCGCACTGCAGCGGGAGTAATGGCTGGTGTCGGGTCAGCGTATCG; CTGCTTTTCTGGGCCTCCAGGGAGTTCTGGTGCGTAAAATTCCTGTGGGCCATGGCGTTC; TTCTGAGGCTCATCAAAGCTCAGTGAGAAGGTGACCGTGCCGCTGCCAAAGATGACCTTC; TGCTTGCATCTGGGCTGCTGCTGAGATCGTTGCTGCTGTGGGAGGGTCAGGGGCTGCTGC; TGCTGCTCTTGCTGGGATAGGGCCAACGGCTGCTGCTGCTTCTGCCTCTCGGGCTGTGGG; AATGGGTCTTCGCTGTTGCTCTTGCTGCTGATGGAGGAGAGGGGGGTTGGATCCTCGTGG; AGCCTCCTAGGCTGCTGNACCGCTTGCTGGAAGACGTTGCTGCNGCGCCACCGTGCCCTG; GCAGCCACTTTAAAGCGTGAGCTGCGGTGCTGCACGCACTCCCCCAGGTGTTTCCGGAAT; GGCTTAAAAGAGGAAGTNCATTTGTTGAAAAAC;
• Restriction Sites: Please inquire
• ACCN: NM_000388
• Insert Size: 3237 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000388.2, NP_000379.2
• RefSeq Size: 4913 bp
• RefSeq ORF: 3237 bp
• Locus ID: 846
• UniProt ID: P41180
• Cytogenetics: 3q13.33-q21.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Summary: The protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]; Transcript Variant: This variant (2) has an alternate 5' UTR exon and lacks an in-frame segment in the CDS, as compared to variant 1. The resulting isoform (2) is shorter and lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.