TSH Receptor (TSHR) (NM_000369) Human Untagged Clone

SKU SC119936

TSHR (untagged)-Human thyroid stimulating hormone receptor (TSHR), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TSH Receptor
• Synonyms: CHNG1; hTSHR-I; LGR3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119936 sequence for NM_000369 edited (data generated by NextGen Sequencing); ATGAGGCCGGCGGACTTGCTGCAGCTGGTGCTGCTGCTCGACCTGCCCAGGGACCTGGGC; GGAATGGGGTGTTCGTCTCCACCCTGCGAGTGCCATCAGGAGGAGGACTTCAGAGTCACC; TGCAAGGATATTCAACGCATCCCCAGCTTACCGCCCAGTACGCAGACTCTGAAGCTTATT; GAGACTCACCTGAGAACTATTCCAAGTCATGCATTTTCTAATCTGCCCAATATTTCCAGA; ATCTACGTATCTATAGATGTGACTCTGCAGCAGCTGGAATCACACTCCTTCTACAATTTG; AGTAAAGTGACTCACATAGAAATTCGGAATACCAGGAACTTAACTTACATAGACCCTGAT; GCCCTCAAAGAGCTCCCCCTCCTAAAGTTCCTTGGCATTTTCAACACTGGACTTAAAATG; TTCCCTGACCTGACCAAAGTTTATTCCACTGATATATTCTTTATACTTGAAATTACAGAC; AACCCTTACATGACGTCAATCCCTGTGAATGCTTTTCAGGGACTATGCAATGAAACCTTG; ACACTGAAGCTGTACAACAATGGCTTTACTTCAGTCCAAGGATATGCTTTCAATGGGACA; AAGCTGGATGCTGTTTACCTAAACAAGAATAAATACCTGACAGTTATTGACAAAGATGCA; TTTGGAGGAGTATACAGTGGACCAAGCTTGCTGGACGTGTCTCAAACCAGTGTCACTGCC; CTTCCATCCAAAGGCCTGGAGCACCTGAAGGAACTGATAGCAAGAAACACCTGGACTCTT; AAGAAACTTCCACTTTCCTTGAGTTTCCTTCACCTCACACGGGCTGACCTTTCTTACCCA; AGCCACTGCTGTGCTTTTAAGAATCAGAAGAAAATCAGAGGAATCCTTGAGTCCTTGATG; TGTAATGAGAGCAGTATGCAGAGCTTGCGCCAGAGAAAATCTGTGAATGCCTTGAATAGC; CCCCTCCACCAGGAATATGAAGAGAATCTGGGTGACAGCATTGTTGGGTACAAGGAAAAG; TCCAAGTTCCAGGATACTCATAACAACGCTCATTATTACGTCTTCTTTGAAGAACAAGAG; GATGAGATCATTGGTTTTGGCCAGGAGCTCAAAAACCCCCAGGAAGAGACTCTACAAGCT; TTTGACAGCCATTATGACTACACCATATGTGGGGACAGTGAAGACATGGTGTGTACCCCC; AAGTCCGATGAGTTCAACCCGTGTGAAGACATAATGGGCTACAAGTTCCTGAGAATTGTG; GTGTGGTTCGTTAGTCTGCTGGCTCTCCTGGGCAATGTCTTTGTCCTGCTTATTCTCCTC; ACCAGCCACTACAAACTGAACGTCCCCCGCTTTCTCATGTGCAACCTGGCCTTTGCGGAT; TTCTGCATGGGGATGTACCTGCTCCTCATCGCCTCTGTAGACCTCTACACTCACTCTGAG; TACTACAACCATGCCATCGACTGGCAGACAGGCCCTGGGTGCAACACGGCTGGTTTCTTC; ACTGTCTTTGCAAGCGAGTTATCGGTGTATACGCTGACGGTCATCACCCTGGAGCGCTGG; TATGCCATCACCTTCGCCATGCGCCTGGACCGGAAGATCCGCCTCAGGCACGCATGTGCC; ATCATGGTTGGGGGCTGGGTTTGCTGCTTCCTTCTCGCCCTGCTTCCTTTGGTGGGAATA; AGTAGCTATGCCAAAGTCAGTATCTGCCTGCCCATGGACACCGAGACCCCTCTTGCTCTG; GCATATATTGTTTTTGTTCTGACGCTCAACATAGTTGCCTTCGTCATCGTCTGCTGCTGT; TATGTGAAGATCTACATCACAGTCCGAAATCCGCAGTACAACCCAGGGGACAAAGATACC; AAAATTGCCAAGAGGATGGCTGTGTTGATCTTCACCGACTTCATATGCATGGCCCCAATC; TCATTCTATGCTCTGTCAGCAATTCTGAACAAGCCTCTCATCACTGTTAGCAACTCCAAA; ATCTTGCTGGTACTCTTCTATCCACTTAACTCCTGTGCCAATCCATTCCTCTATGCTATT; TTCACCAAGGCCTTCCAGAGGGATGTGTTCATCCTACTCAGCAAGTTTGGCATCTGTAAA; CGCCAGGCTCAGGCATACCGGGGGCAGAGGGTTCCTCCAAAGAACAGCACTGATATTCAG; GTTCAAAAGGTTACCCACGACATGAGGCAGGGTCTCCACAACATGGAAGATGTCTATGAA; CTGATTGAAAACTCCCATCTAACCCCAAAGAAGCAAGGCCAAATCTCAGAAGAGTATATG; CAAACGGTTTTGTAA; ; Clone variation with respect to NM_000369.2; 2181 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000369 unedited; CCCCCAGTTCAGATTTTGTAATACAACTTACTATAGNNGGCGGCCGCGCAATTCGGCACG; AGGCAGGGGTGCAGGGCGACGGCTTCGGCCGCACCGCGGGCTAGCCAGGGCTGCGTGCCC; GCCTCTGACCCTCAGCAGAGGTGTCTCTGGCCAGGAGGAGCTGAAGTTCTGCAGGACATT; GGTCCGCCCGCGGACAGTCCACTCCGCGGGGACTTTCTCTGGATAAGGACCTCCTCCACA; GTGGTGAGGTCACAGCCCCTTGGAGCCCTCCCTCTTCCCACCCCTCCCGCTCCCGGGTCT; CCTTTGGCCTGGGGTAACCCGAGGTGCAGAGCTGAGAATGAGGCGATTTCGGAGGATGGA; GAAATAGCCCCGAGTCCCGTGGAAAATGAGGCCGGCGGACTTGCTGCAGCTGGTGCTGCT; GCTCGACCTGCCCAGGGACCTGGGCGGAATGGGGTGTTCGTCTCCACCCTGCGAGTGCCA; TCAGGAGGAGGACTTCAGAGTCACCTGCAAGGATATTCAACGCATCCCCAGCTTACCGCC; CAGTACGCAGACTCTGAAGCTTATTGAGACTCACCTGAGAACTATTCCAAGTCATGCATT; TTCTAATCTGCCCAATATTTCCAGAATCTACGTATCTATAGATGTGACTCTGCAGCAGCT; GGAATCACACTCCTTCTACAATTTGAGTAAAGTGACTCACATAGAAATTCGGAATACCAG; GAACTTAACTTACATAGACCCTGATGCCCTCAAAGAGCTCCCCCTCCTTAAGTTCCTTGG; CATTTTCAACACTGGACTTAACATGTTCCCTTGACCTGACCAAAGTTATTCCACTGATAT; ATNCTTTATACTTNGAAATACAGACGACCCTTACATGACGTCAATCCCTGTGCATGCTTT; TCAGGGACTATGCCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000369 unedited; TTTGGNAGNAATATTTTCTGTTGTGAGTGCAAATTTTAGTAACAATTCTGTTGAAGTCAC; TTTTCTAGGACTCTGTTTCCAAAGCAATTAGTTCAGCTATTCAAGATGAAACACATTATT; TATCAAGATGTGATATTTTGAAATGTCACAGGAGAGAAACACATGACAAAAGGCAACACA; TTTGTTTAATTTCATTAGTTACAAGCGAACTTCTAGGTAAGTCATGATTTTCGATGTTTA; TAATATTTCTATTAAGATGTGCAGGTCTCAATGATAGTGATGAGTGGGTATGGCTGTGGA; GAGATTCCAGGTGAGTCCAGCAGGGACCATCTGTGGGGACCCATTAACGACACTTCACAA; TCTTAAAACAACAAAGAGCTTTTATTTCATGGTGTGGTTTATGAGCATTCAGAAGTAATG; TCATCTCTGCTTGGAGACTTTTCTAGCAGGACATTAGGGGGCTATCATCACAGGTAACCT; GGAGAGTACATCTGTAGATAAAGATCCTGACTATTTTAAGTAAAGAAGAATTATTGGGGA; AAAGAAGCTTTATGTCCAGTGATCAACAGGGCTGGAAAGAGCTGGCAGTTTCAGAAAAGA; AGAAACCTCTCTTGACAGCTTTAGTATCATACCGTGGACTGGAAATGGCAAAAATCATCC; CTTAAGACAAGCGAAGTGTTTTTGAAGTGACTCTTCATAGTGAAGTAGCAACTCTAAATA; GCCAAAATTTGCAACCATCGTTTACTCTTTTATATAAACACGAATCTGTTTTTGGCCTGA; TNATTACAGCTGGGTAGATCTAGTAATAAATGAATGATTCAGATCCAGGCGAGTATGTTT; ACTCAATTGAATTTTTGGCTCAGACTCATTATCAAAAAGTGAACAAGGAAAAATTCCTGG; GTCATGTGTCTAGGCTCCAGGACTATGATCAGCACNCTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000369
• Insert Size: 2295 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000369.2, NP_000360.2
• RefSeq Size: 4410 bp
• RefSeq ORF: 2295 bp
• Locus ID: 7253
• UniProt ID: P16473
• Cytogenetics: 14q31.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Neuroactive ligand-receptor interaction
• Summary: The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]; Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).