GLB1 (NM_000404) Human Untagged Clone

SKU SC119908

GLB1 (untagged)-Human galactosidase, beta 1 (GLB1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GLB1
• Synonyms: EBP; ELNR1; MPS4B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119908 sequence for NM_000404 edited (data generated by NextGen Sequencing); ATGCCGGGGTTCCTGGTTCGCATCCTCCCTCTGCTGCTGGTTCTGCTGCTTCTGGGCCCT; ACGCGCGGCTTGCGCAATGCCACCCAGAGGATGTTTGAAATTGACTATAGCCGGGACTCC; TTCCTCAAGGATGGCCAGCCATTTCGCTACATCTCAGGAAGCATTCACTACTCCCGTGTG; CCCCGCTTCTACTGGAAGGACCGGCTGCTGAAGATGAAGATGGCTGGGCTGAACGCCATC; CAGACGTATGTGCCCTGGAACTTTCATGAGCCCTGGCCAGGACAGTACCAGTTTTCTGAG; GACCATGATGTGGAATATTTTCTTCGGCTGGCTCATGAGCTGGGACTGCTGGTTATCCTG; AGGCCCGGGCCCTACATCTGTGCAGAGTGGGAAATGGGAGGATTACCTGCTTGGCTGCTA; GAGAAAGAGTCTATTCTTCTCCGCTCCTCCGACCCAGATTACCTGGCAGCTGTGGACAAG; TGGTTGGGAGTCCTTCTGCCCAAGATGAAGCCTCTCCTCTATCAGAATGGAGGGCCAGTT; ATAACAGTGCAGGTTGAAAATGAATATGGCAGCTACTTTGCCTGTGATTTTGACTACCTG; CGCTTCCTGCAGAAGCGCTTTCGCCACCATCTGGGGGATGATGTGGTTCTGTTTACCACT; GATGGAGCACATAAAACATTCCTGAAATGTGGGGCCCTGCAGGGCCTCTACACCACGGTG; GACTTTGGAACAGGCAGCAACATCACAGATGCTTTCCTAAGCCAGAGGAAGTGTGAGCCC; AAAGGACCCTTGATCAATTCTGAATTCTATACTGGCTGGCTAGATCACTGGGGCCAACCT; CACTCCACAATCAAGACCGAAGCAGTGGCTTCCTCCCTCTATGATATACTTGCCCGTGGG; GCGAGTGTGAACTTGTACATGTTTATAGGTGGGACCAATTTTGCCTATTGGAATGGGGCC; AACTCACCCTATGCAGCACAGCCCACCAGCTACGACTATGATGCCCCACTGAGTGAGGCT; GGGGACCTCACTGAGAAGTATTTTGCTCTGCGAAACATCATCCAGAAGTTTGAAAAAGTA; CCAGAAGGTCCTATCCCTCCATCTACACCAAAGTTTGCATATGGAAAGGTCACTTTGGAA; AAGTTAAAGACAGTGGGAGCAGCTCTGGACATTCTGTGTCCCTCTGGGCCCATCAAAAGC; CTTTATCCCTTGACATTTATCCAGGTGAAACAGCATTATGGGTTTGTGCTGTACCGGACA; ACACTTCCTCAAGATTGCAGCAACCCAGCACCTCTCTCTTCACCCCTCAATGGAGTCCAC; GATCGAGCATATGTTGCTGTGGATGGGATCCCCCAGGGAGTCCTTGAGCGAAACAATGTG; ATCACTCTGAACATAACAGGGAAAGCTGGAGCCACTCTGGACCTTCTGGTAGAGAACATG; GGACGTGTGAACTATGGTGCATATATCAACGATTTTAAGGGTTTGGTTTCTAACCTGACT; CTCAGTTCCAATATCCTCACGGACTGGACGATCTTTCCACTGGACACTGAGGATGCAGTG; CGCAGCCACCTGGGGGGCTGGGGACACCGTGACAGTGGCCACCATGATGAAGCCTGGGCC; CACAACTCATCCAACTACACGCTCCCGGCCTTTTATATGGGGAACTTCTCCATTCCCAGT; GGGATCCCAGACTTGCCCCAGGACACCTTTATCCAGTTTCCTGGATGGACCAAGGGCCAG; GTCTGGATTAATGGCTTTAACCTTGGCCGCTATTGGCCAGCCCGGGGCCCTCAGTTGACC; TTGTTTGTGCCCCAGCACATCCTGATGACCTCGGCCCCAAACACCATCACCGTGCTGGAA; CTGGAGTGGGCACCCTGCAGCAGTGATGATCCAGAACTATGTGCTGTGACGTTCGTGGAC; AGGCCAGTTATTGGCTCATCTGTGACCTACGATCATCCCTCCAAACCTGTTGAAAAAAGA; CTCATGCCCCCACCCCCGCAAAAAAACAAAGATTCATGGCTGGACCATGTATGA; ; Clone variation with respect to NM_000404.2; 34 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000404 unedited; ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCAC; GAGGGTCAAGTGACGCGAAGCGGCCGGCCTGGGCGCCGACTGCAGAGCCGGGAGGCTGGT; GGTCATGCCGGGGTTCCTGGTTCGCATCCTCCCTCTGCTGCTGGTTCTGCTGCTTCTGGG; CCCTACGCGCGGCTTGCGCAATGCCACCCAGAGGATGTTTGAAATTGACTATAGCCGGGA; CTCCTTCCTCAAGGATGGCCAGCCATTTCGCTACATCTCAGGAAGCATTCACTACTCCCG; TGTGCCCCGCTTCTACTGGAAGGACCGGCTGCTGAAGATGAAGATGGCTGGGCTGAACGC; CATCCAGACGTATGTGCCCTGGAACTTTCATGAGCCCTGGCCAGGACAGTACCAGTTTTC; TGAGGACCATGATGTGGAATATTTTCTTCGGCTGGCTCATGAGCTGGGACTGCTGGTTAT; CCTGAGGCCCGGGCCCTACATCTGTGCAGAGTGGGAAATGGGAGGATTACCTGCTTGGCT; GCTAGAGAAAGAGTCTATTCTTCTCCGCTCCTCCGACCCAGATTACCTGNCAGCTGTGGA; CAAGTGGTTGGGAGTCCTTCTGCCCAGATGAAGCCTCTCCTCTATCAGAATGGAGGNCCA; GTTATAACAGTGCAGGTTGAAAATGAATATGGCAGCTACTTTGCCTGTGANTTTGACTAC; CTGCGCTTCCTGCAGAAGCGCTTTCGNCACCATCTGGNGGATGGATGTGGGTCTGTTTAC; CACTGATGGAGCACATAAAACATTCTGAATGTGGGGCCCTGCAGGGCCTCTACACCACGT; GGACTTTTGAACCAGCAGCAACATACAGATGCTTTCCTAGGCAGAGAAGTGGGAGCCCAA; GCACCTGGAACATTCTGATCTATACTGCTGCTAGACATTGGGGCANCTTACTCCAATCAG; ACCAAGAAGGTTCCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000404 unedited; GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTACAAATTTTATTTAACAAAAAG; GTAACATGATTCTTCCAAAATAAAAATCACTACCACCTTTAAAGCTTCCATTCCAGCCCT; GCAGATATGTATGCACGTTACTGTGCTGTCAGCCCCTCACACATTCCAGGTGGTCCCTGA; AGGTGGGGCTTTGGCACTGCAGGGATGCAGGGAAACCTCAGGTGAAAATGCACATCCTAA; ATTCCTTTTCCATTTCCACATTCCAATCAGTGAAATGTGGCATGACAGGGAGGATCTGTG; AGGTATGTTCAGGGTAGAATCCCTCAAAGACACAGGCTTTCATCATCATACATGGTCCAG; CCATGAATCTTTGTTTTTTTGCGGGGGTGGGGGCATGAGTCTTTTTTCAACAGGTTTGGA; GGGATGATCGTAGGTCACAGATGAGCCAATAACTGGCCTGTCCACGAACGTCACAGCACA; TAGTTCTGGATCATCACTGCTGCAGGGTGCCCACTCCAGTTCCAGCACGGTGATGGTGTT; TGGGGCCGACGTCATCAGGATGTGCTGGGGCACAAACAAGGTCAACTGAGGGCCCCGGGC; TGGCCAATAGCGGCCAAGGTTAAAGCCATTAATCCAGACCTGGCCCTTGGTCCATCCAGG; AAACTGGATAAAGGTGTCCTGGGGCAAGTCTGGGATCCCACTGGGAATGGAGAAGTTCCC; ATATAAAAGCCGGGACGTGTANTTGATGAACTGTGGCCCAGGCTCATCATGTGGCACTGT; ACGGTGGCCCACCCCCCAGGTGCTGCCCCTGATTCTACTGTCCCGGGAAGATTTCCCATC; CTGAGATATTGGACTGAAATAACGTAAAACCAACCCTTAAATCTTGATATATGCCCTATT; CACCCGTCATGTTCCTACAAAAGCCCAAAGGTTCAGTTTCCTTTTCCCGAGGAACACTGG; TTGCTAAGCTCCCTGGATACCCCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000404
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000404.1, NP_000395.1
• RefSeq Size: 2409 bp
• RefSeq ORF: 2034 bp
• Locus ID: 2720
• UniProt ID: P16278
• Cytogenetics: 3p22.3
• Domains: Glyco_hydro_35
• Protein Families: Druggable Genome
• Protein Pathways: Galactose metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Lysosome, Metabolic pathways, Other glycan degradation, Sphingolipid metabolism
• Summary: This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]; Transcript Variant: This variant (1) encodes the predominant isoform (a).