APRT (NM_000485) Human Untagged Clone

CAT#: SC119854

APRT (untagged)-Human adenine phosphoribosyltransferase (APRT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APRT
• Synonyms: AMP; APRTD
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000485, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGACTCCGAGCTGCAGCTGGTTGAGCAGCGGATCCGCAGCTTCCCCGACTTCCCCACCCCAGGCG
  TGGTATTCAGGGACATCTCGCCCGTCCTGAAGGACCCCGCCTCCTTCCGCGCCGCCATCGGCCTCCTGGC
  GCGACACCTGAAGGCGACCCACGGGGGCCGCATCGACTACATCGCAGGCCTAGACTCCCGAGGCTTCCTC
  TTTGGCCCCTCCCTGGCCCAGGAGCTTGGACTGGGCTGCGTGCTCATCCGAAAGCGGGGGAAGCTGCCAG
  GCCCCACTCTGTGGGCCTCCTATTCCCTGGAGTACGGGAAGGCTGAGCTGGAGATTCAGAAAGACGCCCT
  GGAGCCAGGACAGAGGGTGGTCGTCGTGGATGATCTGCTGGCCACTGGTGGAACCATGAACGCTGCCTGT
  GAGCTGCTGGGCCGCCTGCAGGCTGAGGTCCTGGAGTGCGTGAGCCTGGTGGAGCTGACCTCGCTTAAGG
  GCAGGGAGAAGCTGGCACCTGTACCCTTCTTCTCTCTCCTGCAGTATGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000485 unedited
  GCGCCCCGTTGCCGCATATGGGCGGTAGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
  TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC
  GGCACGAGGGGGCTGCCGCTGGCTCTTCGCACGCGGCCATGGCCGACTCCGAGCTGCAGC
  TGGTTGAGCAGCGGATCCGCAGCTTCCCCGACTTCCCCACCCCAGGCGTGGTATTCAGGG
  ACATCTCGCCCGTCCTGAAGGACCCCGCCTCCTTCCGCGCCGCCATCGGCCTCCTGGCGC
  GACACCTGAAGGCGACCCACGGGGGCCGCATCGACTACATCGCAGGCTGAGCTGGAGATT
  CAGAAAGACGCCCTGGAGCCAGGACAGAGGGTGGTCGTCGTGGATGATCTGCTGGCCACT
  GGTGGAACCATGAACGCTGCCTGTGAGCTGCTGGGCCGCCTGCAGGCTGAGGTCCTGGAG
  TGCGTGAGCCTGGTGGAGCTGACCTCGCTTAAGGGCAGGGAGAAGCTGGCACCTGTACCC
  TTCTTCTCTCTCCTGCAGTATGAGTGACCACAGGGCCTCCCAGCCCAACATCTCCAGCTG
  GATCCCAGGGAAATATCAGCCTTGGGCAACTGCAGTGACCAGGGGCACCGGCTGCCCACA
  GGGAACACATTCCTTTGCTGGGGTTCAGCGCCTCTCCTGNGGCTGGAAGTGCCAAAGCCT
  GGGGCANAGCTGTGTTTCAGCCACACTGAACCCAATTACACACAGCGGGAGAACGCAGTA
  NACAGCTTTCCCNACAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCG
  GTCATAGCTGTTNCCCTGACAGATCCCGGGTGGGCATCCTGTGACCCCTCCCAGTGCCTC
  TCCTGGCCCTGNNANNNTGCACTCAGTGCCCACCACCNTNNGTCTATAAANTAAGTGCAT
  CATTTGTCTGACTAGTGTCCTCATAATATATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000485 unedited
  TTAGTTTTAAAACCTTTTTTTTTTTTTTTTGGGGGGATTTCTGTTTACTGCGTTCTCCCG
  CTGTGTGTAATTGGGTTCAGTGTGGCTGAAACACAGCTTTGCCCCAGGCTTTGGCACTTC
  CAGCCCCAGGAGAGGCGCTGAACCCCAGCAAAGGAATGTGTTCCCTGTGGGCAGCCGGTG
  CCCCTGGTCACTGCAGTTGCCCAAGGCTGATATTTCCCTGGGATCCAGCTGGAGATGTTG
  GGCTGGGAGGCCCTGTGGTCACTCATACTGCAGGAGAGAGAAGAAGGGTACAGGTGCCAG
  CTTCTCCCTGCCCTTAAGCGAGGTCAGCTCCACCAGGCTCACGCACTCCAGGACCTCAGC
  CTGCAGGCGGCCCAGCAGCTCACAGGCAGCGTTCATGGTTCCACCAGTGGCCAGCAGATC
  ATCCACGACGACCACCCTCTGTCCTGGCTCCAGGGCGTCTTTCTGAATCTCCAGCTCAGC
  CTGCGATGTAGTCGATGCGGCCCCCGTGGGTCGCCTTCAGGTGTCGCGCCAGGAGGCCGA
  TGGCGGCGCGGAAAGAGGCGGNGTCCTTCAGGACGGGCGAGATGTCCCTGAATACCACGC
  CTGGGGTGGGGAAGTCGGGGAAGCTGCGGATCCGCTGCTCAACCAGCTGCAGCTCGGAGT
  CGGCCATGGGCGCGTGCGAAGAGCCAGCGGCAGCCCCCCTCGTGCCGAATTCGCGGCCGC
  TGCANAAAGAACAGTAGCCTGTATTCTATAGTGTCACCTAAATGAGCTCTGGCTATATAG
  ACCTNCCACCGTACACGCCTTACCGCCCATTTGCGGTCACGGGGNCGGGGGTATTACGAC
  ATTTTGGAAAGTCCCGGTGATTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000485
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000485.2, NP_000476.1
• RefSeq Size: 807 bp
• RefSeq ORF: 543 bp
• Locus ID: 353
• UniProt ID: P07741
• Cytogenetics: 16q24.3
• Domains: Pribosyltran
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).