PMS1 (NM_000534) Human Untagged Clone

SKU SC119824

PMS1 (untagged)-Human PMS1 postmeiotic segregation increased 1 (S. cerevisiae) (PMS1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PMS1
• Synonyms: HNPCC3; hPMS1; MLH2; PMSL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119824 sequence for NM_000534 edited (data generated by NextGen Sequencing); ATGAAACAATTGCCTGCGGCAACAGTTCGACTCCTTTCAAGTTCTCAGATCATCACTTCG; GTGGTCAGTGTTGTAAAAGAGCTTATTGAAAACTCCTTGGATGCTGGTGCCACAAGCGTA; GATGTTAAACTGGAGAACTATGGATTTGATAAAATTGAGGTGCGAGATAACGGGGAGGGT; ATCAAGGCTGTTGATGCACCTGTAATGGCAATGAAGTACTACACCTCAAAAATAAATAGT; CATGAAGATCTTGAAAATTTGACAACTTACGGTTTTCGTGGAGAAGCCTTGGGGTCAATT; TGTTGTATAGCTGAGGTTTTAATTACAACAAGAACGGCTGCTGATAATTTTAGCACCCAG; TATGTTTTAGATGGCAGTGGCCACATACTTTCTCAGAAACCTTCACATCTTGGTCAAGGT; ACAACTGTAACTGCTTTAAGATTATTTAAGAATCTACCTGTAAGAAAGCAGTTTTACTCA; ACTGCAAAAAAATGTAAAGATGAAATAAAAAAGATCCAAGATCTCCTCATGAGCTTTGGT; ATCCTTAAACCTGACTTAAGGATTGTCTTTGTACATAACAAGGCAGTTATTTGGCAGAAA; AGCAGAGTATCAGATCACAAGATGGCTCTCATGTCAGTTCTGGGGACTGCTGTTATGAAC; AATATGGAATCCTTTCAGTACCACTCTGAAGAATCTCAGATTTATCTCAGTGGATTTCTT; CCAAAGTGTGATGCAGACCACTCTTTCACTAGTCTTTCAACACCAGAAAGAAGTTTCATC; TTCATAAACAGTCGACCAGTACATCAAAAAGATATCTTAAAGTTAATCCGACATCATTAC; AATCTGAAATGCCTAAAGGAATCTACTCGTTTGTATCCTGTTTTCTTTCTGAAAATCGAT; GTTCCTACAGCTGATGTTGATGTAAATTTAACACCAGATAAAAGCCAAGTATTATTACAA; AATAAGGAATCTGTTTTAATTGCTCTTGAAAATCTGATGACGACTTGTTATGGACCATTA; CCTAGTACAAATTCTTATGAAAATAATAAAACAGATGTTTCCGCAGCTGACATCGTTCTT; AGTAAAACAGCAGAAACAGATGTGCTTTTTAATAAAGTGGAATCATCTGGAAAGAATTAT; TCAAATGTTGATACTTCAGTCATTCCATTCCAAAATGATATGCATAATGATGAATCTGGA; AAAAACACTGATGATTGTTTAAATCACCAGATAAGTATTGGTGACTTTGGTTATGGTCAT; TGTAGTAGTGAAATTTCTAACATTGATAAAAACACTAAGAATGCATTTCAGGACATTTCA; ATGAGTAATGTATCATGGGAGAACTCTCAGACGGAATATAGTAAAACTTGTTTTATAAGT; TCCGTTAAGCACACCCAGTCAGAAAATGGCAATAAAGACCATATAGATGAGAGTGGGGAA; AATGAGGAAGAAGCAGGTCTTGAAAACTCTTCGGAAATTTCTGCAGATGAGTGGAGCAGG; GGAAATATACTTAAAAATTCAGTGGGAGAGAATATTGAACCTGTGAAAATTTTAGTGCCT; GAAAAAAGTTTACCATGTAAAGTAAGTAATAATAATTATCCAATCCCTGAACAAATGAAT; CTTAATGAAGATTCATGTAACAAAAAATCAAATGTAATAGATAATAAATCTGGAAAAGTT; ACAGCTTATGATTTACTTAGCAATCGAGTAATCAAGAAACCCATGTCAGCAAGTGCTCTT; TTTGTTCAAGATCATCGTCCTCAGTTTCTCATAGAAAATCCTAAGACTAGTTTAGAGGAT; GCAACACTACAAATTGAAGAACTGTGGAAGACATTGAGTGAAGAGGAAAAACTGAAATAT; GAAGAGAAGGCTACTAAAGACTTGGAACGATACAATAGTCAAATGAAGAGAGCCATTGAA; CAGGAGTCACAAATGTCACTAAAAGATGGCAGAAAAAAGATAAAACCCACCAGCGCATGG; AATTTGGCCCAGAAGCACAAGTTAAAAACCTCATTATCTAATCAACCAAAACTTGATGAA; CTCCTTCAGTCCCAAATTGAAAAAAGAAGGAGTCAAAATATTAAAATGGTACAGATCCCC; TTTTCTATGAAAAACTTAAAAATAAATTTTAAGAAACAAAACAAAGTTGACTTAGAAGAG; AAGGATGAACCTTGCTTGATCCACAATCTCAGGTTTCCTGATGCATGGCTAATGACATCC; AAAACAGAGGTAATGTTATTAAATCCATATAGAGTAGAAGAAGCCCTGCTATTTAAAAGA; CTTCTTGAGAATCATAAACTTCCTGCAGAGCCACTGGAAAAGCCAATTATGTTAACAGAG; AGTCTTTTTAATGGATCTCATTATTTAGACGTTTTATATAAAATGACAGCAGATGACCAA; AGATACAGTGGATCAACTTACCTGTCTGATCCTCGTCTTACAGCGAATGGTTTCAAGATA; AAATTGATACCAGGAGTTTCAATTACTGAAAATTACTTGGAAATAGAAGGAATGGCTAAT; TGTCTCCCATTCTATGGAGTAGCAGATTTAAAAGAAATTCTTAATGCTATATTAAACAGA; AATGCAAAGGAAGTTTATGAATGTAGACCTCGCAAAGTGATAAGTTATTTAGAGGGAGAA; GCAGTGCGTCTATCCAGACAATTACCCATGTACTTATCAAAAGAGGACATCCAAGACATT; ATCTACAGAATGAAGCACCAGTTTGGAAATGAAATTAAAGAGTGTGTTCATGGTCGCCCA; TTTTTTCATCATTTAACCTATCTTCCAGAAACTACATGA; ; Clone variation with respect to NM_000534.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000534 unedited; GCAACGCCATTCGCACGAGGGCGCATCGGTTGAGACGCTGGCTGCTTGCGGCTAGTGGAT; GGTAATTGCCTGCCTCGCGCTAGCAGGAAGCTGCTCTGTTAAAAGCGAAAATGAAACAAT; TGCCTGCGGCAACAGTTCGACTCCTTTCAAGTTCTCAGATCATCACTTCGGTGGTCAGTG; NTTAAAATAGCTTATTGAAAACTCCTTGGATGCTGGTGCCACAAGCGTAGATGTTAAACT; GGAGAACTATGGATTTGATAAAATTGAGGTGCGAGATAACGGGGAGGGTATCAAGGCTGT; TGATGCACCTGTAATGGCAATGAAGTACTACACCTCAAAAATAAATAGTCATGAAGATCT; TGAAAATTTGACAACTTACGGTTTTCGTGGAGAAGCCTTGGGGTCAATTTGTTGTATAGC; TGAGGTTTTAATTACAACAAGAACGGCTGCTGATAATTTTAGCACCCAGTATGTTTTAGA; TGGCAGTGGCCACATACTTTCTCAGAAACCTTCACATCTTGGTCAAGGTACAACTGTAAC; TGCTTTAAGATTATTTAAGAATCTACCTGTAAGAAAGCAGTTTTACTCAACTGCAAAAAA; ATGTAAAGATGAAATAAAAAAGATCCAAGATCTCCTCATGAGCTTTGGTATCCTTAAACC; TGACTTAAGGATTGTCTTTGTACATAACAAGGCAGTTATTTGGCAGAAAAGCAGAGTATC; AGATCACAAGATGGCTCTCATGTCAGTTCTGGGGACTGCTGTTATGAACAATATGAAATC; CTTTCAGTACCACTCTGAAGAATCTCAGATTTATCTCAGTGAATTTCTTCCAAGTGTGAT; GCAGACCACTCTTTCCTAATCTTTCACACCAAAAAGATTTCAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000534 unedited; TGGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAATAGTCTATATTA; GTTTATTTACGTTTTCTACAATACGTGGAACTTTTTTCAATATAAAAACAAGTCAGTGAA; TCCTCATTTAAAAAATAACCATGTGACACATAATACAAAGATAATTTAAAACCAGACTCA; TGCTGTTTTATGACAGAACCAATTTCAATGGTAACTAATCTTCTTAAACATATTTAATCA; TGTAGTTTCTGGAAGATAGGTTAAATGATGAAAAAATGGGCGACCATGAACACACTCTTT; AATTTCATTTCCAAACTGGTGCTTCATTCTGTAGATAATGTCTTGGATGTCCTCTTTTGA; TAAGTACATGGGTAATTGTCTGGATAGACGCACTGCTTCTCCCTCTAAATAACTTATCAC; TTTGCGAGGTCTACATTCATAAACTTCCTTTGCATTTCTGTTTAATATAGCATTAAGAAT; TTCTTTTAAATCTGCTACTCCATAGAATGGGAGACAATTAGCCATTCCTTCTATTTCCAA; GTAATTTTCAGTAATTGAAACTCCTGGTATCAATTTTATCTTGAAACCATTCGCTGTAAG; ACGAGGATCAGACAGGTAAGTTGATCCACTGTATCTTTGGTCATCTGCTGTCATTTTATA; TAAAACGTCTAAATAATGAGATCCATTAAAAAGACTCTCTGTTAACATAATTGGCTTTTC; CAGTGGCTCTGCANGAAAGTTATGATTCTCAAGAAAGTCTTTAATAGCANGGCTTCTTCT; ACTCATATGGATTTATAAACATACCTCTGTTTGGNATGTCATTAGCCATGCATCAAGAAC; CTGAGATGTGGATCAAGCGGTTCATCCTTCTCTCTAGNCACTTGGTTTGTTCTTAAATTA; TTTTTAGTTTTATAGAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000534
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000534.3, NP_000525.1
• RefSeq Size: 3239 bp
• RefSeq ORF: 2799 bp
• Locus ID: 5378
• UniProt ID: P54277
• Cytogenetics: 2q32.2
• Domains: DNA_mis_repair, HATPase_c, HMG
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a protein belonging to the DNA mismatch repair mutL/hexB family. This protein is thought to be involved in the repair of DNA mismatches, and it can form heterodimers with MLH1, a known DNA mismatch repair protein. Mutations in this gene cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other genes involved in the HNPCC phenotype, which is also known as Lynch syndrome. [provided by RefSeq, Jul 2008]